39 results about "Exogenous factor" patented technology

The invention discloses a method and a system for continuous attention assessment. The method comprises the following steps: when a tested person is in a simulated task state, the behavior index data, the eye movement index data and the electroencephalogram index data of the tested person are acquired; the above acquired data are input into a preset comprehensive evaluation model, and the output of the comprehensive evaluation model is adopted as one of a plurality of assessment grades. The comprehensive evaluation model is obtained in advance through the following mode: an ideal evaluation index table of the plurality of assessment grades is obtained based on the test data of tested people of a preset number in the actual task state, and a multi-input and single-output relation model is obtained through the neural network learning process under the supervision of the ideal evaluation index table. According to the invention, the continuous attention is fully fused with specific scene task characteristics, and indexes of three dimensions are adopted. A more objective and more accurate continuous attention assessment scheme is established from the representation layer to the mechanism layer, and from exogenous factors to endogenous factors.

Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.

Systems and methods are disclosed for evaluating a property valuation. In some implementations, market information and transactional information for a group of properties is accessed. The market information can include property-specific factors, locational factors, and market sales factors. The transactional information can include exogenous factors that can influence a property evaluator. Statistical information relating to the market values of the properties and the property valuations can be calculated. An estimate of the degree of certainty for a property valuation can be determined based at least partly on the calculated statistical information.

The invention relates to a non-exogenous induced factor integrated porcine induced pluripotent stem cell and its construction method. The method comprises the steps of: A) infecting a porcine induced pluripotent stem cell with a CreER lentiviral vector, inducing the infected stem cell with 4OH-Tamoxifen, cutting off loxp-carrying exogenous factors Oct4, Sox2, Klf4 and c-Myc by CreER; and B) screening out a stem cell that does not express green fluorescent protein by means of FACS, i.e. the non-exogenous induced factor integrated porcine induced pluripotent stem cell. The non-exogenous induced factor integrated porcine induced pluripotent stem cell obtained in the invention can be applied in nuclear transfered cloned pigs or transgenic pigs, thus greatly reducing the carcinogenic risk of exogenous induced factors, especially c-Myc.

The invention provides a schizochytrium limacinum strain as well as a mutagenesis method and an application thereof. In the prior art, although how to improve the content of DHA in a strain is reported, since the consideration factor is often single, how to significantly improve the content of DHA in the strain is not comprehensively explored which also is a key to achieve the industrialization of schizochytrium limacinum. Schizochytrium limacinum TC5-2 is obtained by virtue of an ion beam mutagenesis method, by combining a culture medium, optimizing a fermentation process and adding an exogenous factor clethodim, the content of DHA in the strain is significantly improved which is conductive to achieving the industrialization of production of DHA by virtue of schizochytrium limacinum.

The invention relates to the field of technical research of stem cells and provides a preparation method of freeze-dried powder containing stem-cell active factors excreted by human mesenchymal stem cells. Provided human adipose-derived stem cell raw materials are sufficient in source, safe and convenient to extract and safe and reliable in use and do not produce any untoward effect. The preparation method specifically comprises the following steps of exogenous factor detection, primary isolation, culture and cell passage of adipose-derived stem cells, identification of mesenchymal stem cells,collection of supernate, preparation of the freeze-dried powder, detection on the supernate and active factors of the stem cells of the freeze-dried powder and solvent preparation and filling. The prepared freeze-dried powder is easy to store and transport compared with the supernate, the activity of the factors is effectively saved, the freeze-dried powder can be applied to the beauty industry,the cell factor content is high, the moisture content in the freeze-dried powder is low, and accordingly the freeze-dried powder is easy to store. The freeze-dried powder prepared by adopting the preparation method is adopted after scalding, and the heal rate is high when the compound prepared from biological polysaccharide gel, collagen, folic acid and sterile ultrapure water is applied to wounds.

The invention discloses a method of non-exogenous induction of a pluripotent stem cell to be a GABA neuron. The method includes the following steps of 1 cultivating a pluripotent stem cell in a Matrigel or a Vitronectin matrigel system to the density of 60 % -80 %; 2 digesting the pluripotent stem cell obtained in step 1 with Dispase or EDTA (Ethylene Diamine Tetraacetic Acid), differentiating the suspension culture through neural induction on that day, and recording the day as the 0 day; 3 adding neural induction differentiation solution for further cultivation when a spherical cell formed by the pluripotent stem cell can be observed; 4 performing adherence on the seventh day; 5 adding SHH or a small molecule compound Purmophine on the tenth day for continuous induction; 6 blowing off a formed neural tube structure on the sixteenth day and continuing to cultivate with the neural induction solution; 7 obtaining the GABA neuron on the twenty-second day. The method of non-exogenous induction of the pluripotent stem cell to be the GABA neuron can significantly improve cultivation solution complication and low differentiation rate and eliminate the interference of exogenous factors.

The invention provides methods and compositions for local manipulation of regenerative processes via exogenous factor delivery.

The invention provides a method for producing avian influenza virus (H5 subtype and H9 subtype) bivalent inactivated vaccine by use of a MDCK cell line. The method comprises the following steps: (1) passage and culture of the MDCK cell line; (2) virus inoculation and reproduction; (3) inactivation and harvesting of virus liquid; and (4) preparation of the vaccine. The method for producing avian influenza virus inactivated vaccine by use of the MDCK cell line has the advantages that exogenous factor pollution is avoided, large-scale production is easy to realize, the virus antigen stability can be maintained well and the like; and moreover, the development period and production period of the vaccine can be shortened, and the ability of emergency supply of vaccine in outburst epidemic situation is realized.

The present invention relates to treatment methods and methods for sustained delivery of one or more exogenous factors to desired nervous system sites. In certain embodiments, the invention relates to the use of biodegradable microspheres to deliver exogenous factors, such as the morphogenic factor, sonic hedgehog (Shh), to the site of spinal cord injury. In certain embodiments, the Shh-releasing microspheres are administered together with stem cells, which may be spinal cord neural stem cells. In certain embodiments, the invention relates to regrowth of neural cells in both the central and peripheral nervous systems.

The invention provides a preparation method and application of induction type neural stem cells. The preparation method of the induction type neural stem cells comprises the steps of: carrying out overexpression of exogenous factors Ascl1, Neurog2, Pax6, Hes1, Id1, Brn2, c-Myc and Klf4 in sustentacular cells of testis, and culturing in a base culture fluid containing cell growth factors to obtain the induction type neural stem cells. According to the preparation method of the induction type neural stem cells, sustentacular cells of testis derived from the mesoblast are reprogrammed to be neural stem cells derived from the ectoderm, the neural stem cells obtained in the reprogramming manner are proved to have physiological functions in vivo or in vitro, and the cells are safer than pluripotent stem cells for transplantation.

The present disclosure is directed to a framework for modelling the effects on exogenous factors on an operating plan that captures how a business would react to variability of exogenous factors as well as the effect of simultaneously implementing various operating decisions. The framework can also generate optimal operating plans given variability of exogenous factors and reactive business decisions. The framework offers strategic risk management decision support for a business producing goods that are heavily exposed to raw material prices in the commodity markets. The framework accounts for both exogenous factors as well as operational decisions. Specifically, for example, the framework can model corporate earnings and the impact of expenses, revenue, and profit / loss from financial instruments given uncertain exogenous factors while integrating the effects on earnings of operational decisions made in relation to physical assets, real options, and of finished goods selection.