40 results about "Exogenous factor" patented technology

Methods are described for mapping a pathway of differentiation of a population of embryonic cells which includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies which are then exposed to one or more exogenous factors to enrich a culture for a particular cell type. The differentiated cells may be used for treating a medical condition in a human. Kits for determining differentiation pathways and screening exogenous factors for their utility in differentiation are provided.

A method for targeting, treating, or diagnosing malignant mammalian tumor cells, comprising administering an effective amount of a β1,6-branched oligosaccharide specific binding agent to the mammal. As a treatment, the binding agent may be intrinsically cytotoxic, initiate an endogenous cytotoxic cascade, or play a role in a cytotoxic cascade involving exogenous factors. A preferred binding agent is Bordetella pertussis, which is both specific for the β1,6-branched oligosaccharide and well tolerated. Genetically engineered organisms may also be employed. Pharmaceutical compositions may also serve as binding agents.

The invention provides methods and compositions for local manipulation of regenerative processes via exogenous factor delivery.

The invention discloses a method and a system for continuous attention assessment. The method comprises the following steps: when a tested person is in a simulated task state, the behavior index data, the eye movement index data and the electroencephalogram index data of the tested person are acquired; the above acquired data are input into a preset comprehensive evaluation model, and the output of the comprehensive evaluation model is adopted as one of a plurality of assessment grades. The comprehensive evaluation model is obtained in advance through the following mode: an ideal evaluation index table of the plurality of assessment grades is obtained based on the test data of tested people of a preset number in the actual task state, and a multi-input and single-output relation model is obtained through the neural network learning process under the supervision of the ideal evaluation index table. According to the invention, the continuous attention is fully fused with specific scene task characteristics, and indexes of three dimensions are adopted. A more objective and more accurate continuous attention assessment scheme is established from the representation layer to the mechanism layer, and from exogenous factors to endogenous factors.

Anti-TRAIL-R1 and R2 antibodies or functional fragments thereof, having at least one property selected from the following (a) to (c) of: (a) having activity to induce apoptosis in carcinoma cells expressing TRAIL-R1 and / or TRAIL-R2; (b) not having effect on normal human cells expressing TRAIL-R1 and / or TRAIL-R2; and (c) not inducing human hepatocyte toxicity, and an anti-TRAIL-R1 and R2 antibodies or functional fragments thereof, having the following properties: having activity to induce apoptosis in carcinoma cells independently of exogenous factors and as a monomer of an antibody.

Systems and methods are disclosed for evaluating a property valuation. In some implementations, market information and transactional information for a group of properties is accessed. The market information can include property-specific factors, locational factors, and market sales factors. The transactional information can include exogenous factors that can influence a property evaluator. Statistical information relating to the market values of the properties and the property valuations can be calculated. An estimate of the degree of certainty for a property valuation can be determined based at least partly on the calculated statistical information.

The invention discloses an evaluation index equilibrium state analysis method based on Bayesian causal network, comprising the following steps: establishing a system evaluation index system, and determining exogenous factors affecting the system; obtaining a corresponding endogenous variable set from the evaluation index, The exogenous input variable set is obtained from the exogenous influencing factors of the system, and the output variable set is obtained from the evaluation results; a three-layer Bayesian causal network structure is constructed according to the endogenous variable set, exogenous input variable set and output variable set, and the conditional The independence test finds the causal relationship between variables; conducts system dynamics modeling and simulation calculations to obtain the equilibrium state of each variable according to the Bayesian causal network structure and the causal relationship between variables; maps the equilibrium state of each variable to the evaluation Indexes, the equilibrium state of each evaluation index under the constraints of exogenous conditions is obtained. The invention has the following advantages: it can effectively discover the causal relationship between the evaluation indexes in the complex system and obtain the equilibrium state of the evaluation indexes under different conditions.

The invention provides a serum-free cell cryopreservation solution as well as a preparation method and application thereof, and relates to the technical field of cell culture. The cryopreservation solution comprises a serum-free medium, dimethyl sulfoxide, hydroxyethyl starch, catechin, sodium tetraborate, sodium hyaluronate and vitamin C, and does not contain bovine serum or other protein components, thereby avoiding the introduction of exogenous factors causing cell seed contamination. The cryopreservation solution has low toxicity and remarkable cryopreservation effect, and the cell survival rate after freezing for 3 months reaches 95% or above. In addition, a use method of the serum-free cell cryopreservation solution is simple to operate, no complicated program for cooling is required, and the serum-free cell cryopreservation solution can be widely used for cryopreservation of various mammalian cells.

The invention relates to a non-exogenous induced factor integrated porcine induced pluripotent stem cell and its construction method. The method comprises the steps of: A) infecting a porcine induced pluripotent stem cell with a CreER lentiviral vector, inducing the infected stem cell with 4OH-Tamoxifen, cutting off loxp-carrying exogenous factors Oct4, Sox2, Klf4 and c-Myc by CreER; and B) screening out a stem cell that does not express green fluorescent protein by means of FACS, i.e. the non-exogenous induced factor integrated porcine induced pluripotent stem cell. The non-exogenous induced factor integrated porcine induced pluripotent stem cell obtained in the invention can be applied in nuclear transfered cloned pigs or transgenic pigs, thus greatly reducing the carcinogenic risk of exogenous induced factors, especially c-Myc.

The invention provides a schizochytrium limacinum strain as well as a mutagenesis method and an application thereof. In the prior art, although how to improve the content of DHA in a strain is reported, since the consideration factor is often single, how to significantly improve the content of DHA in the strain is not comprehensively explored which also is a key to achieve the industrialization of schizochytrium limacinum. Schizochytrium limacinum TC5-2 is obtained by virtue of an ion beam mutagenesis method, by combining a culture medium, optimizing a fermentation process and adding an exogenous factor clethodim, the content of DHA in the strain is significantly improved which is conductive to achieving the industrialization of production of DHA by virtue of schizochytrium limacinum.

The invention relates to the technical field of biology, in particular to a method for producing a human diploid cell encephalitis B inactivated vaccine. The method sequentially comprises the following steps of: resuscitating and subculturing a human diploid cell strain; culturing the human diploid cell strain by using a multi-stage bioreactor; inoculating an encephalitis B virus strain, and performing virus multiplication culture; harvesting a virus culture solution; and inactivating the virus culture solution, performing ultrafiltration, purifying, adding a glycoprotein protective agent, and preparing the vaccine. Human diploid cells are used as a cell matrix, so that the vaccine is safe and is free from exogenous factor pollution; the human diploid cells are cultured by the bioreactor through stage-by-stage linear amplification, so that the large-scale production of the vaccine with low cost, high quality, safety and stability is easy to implement; and the titer of the virus harvested solution is subjected to sampling inspection every day, so that the high expression of the harvested virus culture is ensured, and production quality is ensured.

The invention relates to the field of technical research of stem cells and provides a preparation method of freeze-dried powder containing stem-cell active factors excreted by human mesenchymal stem cells. Provided human adipose-derived stem cell raw materials are sufficient in source, safe and convenient to extract and safe and reliable in use and do not produce any untoward effect. The preparation method specifically comprises the following steps of exogenous factor detection, primary isolation, culture and cell passage of adipose-derived stem cells, identification of mesenchymal stem cells,collection of supernate, preparation of the freeze-dried powder, detection on the supernate and active factors of the stem cells of the freeze-dried powder and solvent preparation and filling. The prepared freeze-dried powder is easy to store and transport compared with the supernate, the activity of the factors is effectively saved, the freeze-dried powder can be applied to the beauty industry,the cell factor content is high, the moisture content in the freeze-dried powder is low, and accordingly the freeze-dried powder is easy to store. The freeze-dried powder prepared by adopting the preparation method is adopted after scalding, and the heal rate is high when the compound prepared from biological polysaccharide gel, collagen, folic acid and sterile ultrapure water is applied to wounds.

The invention provides a preparation method and application of induction type neural stem cells. The preparation method of the induction type neural stem cells comprises the steps of: carrying out overexpression of exogenous factors Ascl1, Neurog2, Pax6, Hes1, Id1, Brn2, c-Myc and Klf4 in sustentacular cells of testis, and culturing in a base culture fluid containing cell growth factors to obtain the induction type neural stem cells. According to the preparation method of the induction type neural stem cells, sustentacular cells of testis derived from the mesoblast are reprogrammed to be neural stem cells derived from the ectoderm, the neural stem cells obtained in the reprogramming manner are proved to have physiological functions in vivo or in vitro, and the cells are safer than pluripotent stem cells for transplantation.

The invention provides a culture medium for differentiating neural stem cells into oligodendrocyte progenitor cells. The culture medium does not contain exogenous factors, so that pollution of the exogenous factors is avoided, and the oligodendrocyte progenitor cells can be efficiently differentiated. The invention further provides a method for preparing the oligodendrocyte progenitor cells by using the culture medium. By use of the method provided by the invention, the differentiation efficiency of the oligodendrocyte progenitor cells is improved on the premise of increasing the yield of theoligodendrocyte progenitor cells.

The invention relates to a method for purifying a slow virus. The method comprises the following steps: (S10) providing a cell culture containing the slow virus; (S20) carrying out centrifugal concentration on the cell culture, and filtering to obtain virus suspension; (S30) purifying the virus suspension by virtue of anion exchange chromatography; and (S40) purifying the virus suspension by virtue of gel filtration chromatography. A process for purifying the slow virus is easy to amplify, can be used for processing large amounts of virus liquid produced by cell factories or bioreactors on a large scale, can meet production requirements of slow virus liquid, is stable and is good in repeatability; furthermore, the purified slow virus liquid prepared by virtue of the method is not polluted by exogenous factors, is low in residues of cell host protein and host DNA, good in safety, and high in purity and titer and can completely meet requirements of clinic gene therapy.

The invention discloses a method of non-exogenous induction of a pluripotent stem cell to be a GABA neuron. The method includes the following steps of 1 cultivating a pluripotent stem cell in a Matrigel or a Vitronectin matrigel system to the density of 60 % -80 %; 2 digesting the pluripotent stem cell obtained in step 1 with Dispase or EDTA (Ethylene Diamine Tetraacetic Acid), differentiating the suspension culture through neural induction on that day, and recording the day as the 0 day; 3 adding neural induction differentiation solution for further cultivation when a spherical cell formed by the pluripotent stem cell can be observed; 4 performing adherence on the seventh day; 5 adding SHH or a small molecule compound Purmophine on the tenth day for continuous induction; 6 blowing off a formed neural tube structure on the sixteenth day and continuing to cultivate with the neural induction solution; 7 obtaining the GABA neuron on the twenty-second day. The method of non-exogenous induction of the pluripotent stem cell to be the GABA neuron can significantly improve cultivation solution complication and low differentiation rate and eliminate the interference of exogenous factors.

The invention provides a method for increasing a fermentation yield of artemisinic acid. The method comprises the following steps: using an engineered strain of Saccharomyces cerevisiae capable of producing the artemisinic acid as a production strain; and fermenting in a fermentation culture medium containing a lactose hydrolysate capable of hydrolyzing into glucose and galactose, mevalonic acid and / or citral and other exogenous factors. The method provided by the invention increases the fermentation yield of artemisinic acid, reduces the fermentation cost of artemisinic acid, adopts a simpleand feasible technical solution, and has advantages of environmental protection, high genetic stability of the strain, and easy industrial production and application.

The invention discloses a high yield baculovirus insect cell line Super Spodoptera frugiperda a (SuperSf-a) with an accession number of CCTCCNo.C2014100; the high yield baculovirus insect cell line has the ability of high yield production of baculovirus, can efficiently express an exogenous gene, has the characteristics of high baculovirus infection susceptibility and the like, an exogenous viral protein or a virus like particle product expressed by the cell line has a strong biological activity, and meets requirements of vaccine production; by exogenous virus detection, mycoplasma detection, sterility detection and exogenous factor detection, various indices of the cell line are in accordance with the requirements of cell matrix used for vaccine production; and the cell line can be used in baculovirus and vaccine production.

The invention discloses a method and system for assessing the update ability of spatial working memory. The method includes the steps of: obtaining the behavior index data and EEG index data of the tested person when the tested person is in a simulated task state; The data is used as the input of the preset comprehensive evaluation model, and the output of the comprehensive evaluation model is one of multiple assessment levels; the comprehensive evaluation model is obtained in advance in the following way: based on the test data of a predetermined number of tested personnel in the actual task state, the obtained An ideal evaluation index table corresponding to the assessment level, and under the supervision of the ideal evaluation index table, a multi-input and single-output relationship model is obtained through neural network learning. The implementation of the present invention fully integrates the updating ability of spatial working memory with the characteristics of specific scene tasks, adopts two-dimensional indicators, goes deep from the appearance level to the mechanism level, and from exogenous factors to endogenous factors, establishes a more objective and accurate Spatial working memory update ability assessment scheme.

The invention provides methods and compositions for local manipulation of regenerative processes via exogenous factor delivery.

The invention provides a method for producing avian influenza virus (H5 subtype and H9 subtype) bivalent inactivated vaccine by use of a MDCK cell line. The method comprises the following steps: (1) passage and culture of the MDCK cell line; (2) virus inoculation and reproduction; (3) inactivation and harvesting of virus liquid; and (4) preparation of the vaccine. The method for producing avian influenza virus inactivated vaccine by use of the MDCK cell line has the advantages that exogenous factor pollution is avoided, large-scale production is easy to realize, the virus antigen stability can be maintained well and the like; and moreover, the development period and production period of the vaccine can be shortened, and the ability of emergency supply of vaccine in outburst epidemic situation is realized.

The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.

The invention relates to a fat stem cell membrane sheet, particularly relates to a method for constructing a fat stem cell membrane sheet and application, and belongs to the fields of tissue engineering and regenerative medicine. The method comprises the following steps: step 1) selecting and using fat stem cells as seed cells and constructing a cell membrane sheet; step 2) enabling endometrial cells to be applied to induced cocultured cells; and step 3) constructing the fat stem cell membrane sheet by adopting chemical induction and coculture modes. Exogenous factor (TGF-beta 1, EGF, PDGF-BBand 17-beta estradiol) are added and are compounded with the endometrial cells to perform coculture by utilizing a membrane sheet culture medium, human fat stem cells are induced to form membranes; the expression quantity of each epithelial marked mRNA of the membrane sheet which is induced by the membrane sheet culture system is up-regulated; and immunofluorescence shows that protein CK18 expresses positive and prompts that part of stem cells are transformed into epithelial cells. The membrane sheet induced by the system is more effective in the aspect of repairing endometrial epithelium.

The present invention relates to treatment methods and methods for sustained delivery of one or more exogenous factors to desired nervous system sites. In certain embodiments, the invention relates to the use of biodegradable microspheres to deliver exogenous factors, such as the morphogenic factor, sonic hedgehog (Shh), to the site of spinal cord injury. In certain embodiments, the Shh-releasing microspheres are administered together with stem cells, which may be spinal cord neural stem cells. In certain embodiments, the invention relates to regrowth of neural cells in both the central and peripheral nervous systems.

The invention relates to a human parainfluenza virus type 3 (HPIV-3) wild strain and an application thereof. The preservation number of the wild strain is CCTCC NO:V201911, the wild strain has HN protein and F protein of an HPIV-3 virus, and the wild strain can be applied to an HPIV-3 virus infected mouse model and an HPIV-3 neutralizing antibody detection experiment, and can be applied to preparation of vaccines for preventing parainfluenza type 3. The human parainfluenza virus type 3 wild strain disclosed by the invention is a cloned purified high-titer HPIV3LZ1728C19 virus strain free fromspecific exogenous factor plaque formation, and a human parainfluenza virus type 3 HN protein subunit vaccine prepared from the virus can effectively prevent parainfluenza virus type 3 infection.

The invention provides a preparation method and application of induction type neural stem cells. The preparation method of the induction type neural stem cells comprises the steps of: carrying out overexpression of exogenous factors Ascl1, Neurog2, Pax6, Hes1, Id1, Brn2, c-Myc and Klf4 in sustentacular cells of testis, and culturing in a base culture fluid containing cell growth factors to obtain the induction type neural stem cells. According to the preparation method of the induction type neural stem cells, sustentacular cells of testis derived from the mesoblast are reprogrammed to be neural stem cells derived from the ectoderm, the neural stem cells obtained in the reprogramming manner are proved to have physiological functions in vivo or in vitro, and the cells are safer than pluripotent stem cells for transplantation.

The invention discloses a traditional Chinese medicine preparation for treating calcaneodynia. The traditional Chinese medicine preparation is prepared from the following crude drugs in part by weight: 6 parts of angelica sinensis, 6 parts of rhizome of Sichuan lovage, 6 parts of rhizome of drynaria, 6 parts of licorice, 10 parts of white peony root, 30 parts of acanthopanax bark, 30 parts of raw coix seed, 15 parts of cajeput oil, 8 parts of achyranthes root, 10 parts of solanum mammosum root, 10 parts of honeylocust fruit, 5 parts of airpotato yam vine, 5 parts of shark liver, and 5 parts of semen thlaspi. Clinical trials show that the traditional Chinese medicine preparation has the efficacies of tonifying the kidney, strengthening bones, activating collaterals, removing obstruction, removing dampness, alleviating pain and removing exogenous factor by selecting proper crude drugs and the ratio thereof, is extremely high in the cure rate of calcaneodynia, and is safe, and free of toxic and side effects.

The invention discloses a glass gene chip for detecting human DNA repair enzyme genetic polymorphism, which comprises a glass substrate subjected to aldehyde treatment, and oligonucleotide probe, control and blank spot coatings distributed on the glass substrate in an array, wherein the locus coatings comprise six strips of different-locus DNA repair enzyme gene-specific detection probes, one strip of positive control, three strips of negative control and blank spot sample solution control. The detection probes and control probes of the glass gene chip all take poly(dT)15 as connecting arms, and amino-group-containing six-carbon groups are added into the 5' ends of the probes. The invention also discloses the design of an upstream primer and a downstream primer for the amplification of DNA samples in human whole blood. The chip of the invention can quickly and highly-efficiently perform simultaneous detection on the polymorphism of human DNA repair enzyme genes at XRCC1Arg194Trp locus, Arg399Gln locus and XPDAsp321Asn locus so as to know the capacity of organisms in repairing DNA damage caused by endogenic factors and exogenous factors, and has great significance for the judgment of risks that individuals suffer from malignant tumors, guidance for patients with the tumors to select proper chemotherapeutical medicaments and the execution of individualized therapy.

The present disclosure is directed to a framework for modelling the effects on exogenous factors on an operating plan that captures how a business would react to variability of exogenous factors as well as the effect of simultaneously implementing various operating decisions. The framework can also generate optimal operating plans given variability of exogenous factors and reactive business decisions. The framework offers strategic risk management decision support for a business producing goods that are heavily exposed to raw material prices in the commodity markets. The framework accounts for both exogenous factors as well as operational decisions. Specifically, for example, the framework can model corporate earnings and the impact of expenses, revenue, and profit / loss from financial instruments given uncertain exogenous factors while integrating the effects on earnings of operational decisions made in relation to physical assets, real options, and of finished goods selection.