49 results about "Artemisinic acid" patented technology

The invention discloses a method for preparing artemisinic acid, which is convenient to operate and has little pollution. The method is as follows: 1) crushing dry artemisia annua to 20-80 mesh crumbs, and placing the crumbs in a CO2 supercritical extracting tank which is filled with liquid CO2 for extraction, wherein the flow rate is 1-5ml/min/g raw material, the extraction temperature is 30-50DEG C, the pressure is 15-30Mpa, and the extraction time is 1-3h, and the extract is obtained after dissolving out; 2) dissolving the extract with a sodium bicarbonate solution, adding petroleum ether to the solution after filtration, adjusting to ph3-5 with hydrochloric acid solution, collecting a petroleum ether extraction liquid, decolorizing the extraction liquid with activated carbon, concentrating the extraction liquid to a small volume and crystallizing, and re-crystalizing crystals with anhydrous methanol to obtain the artemisinic acid. The artemisinic acid sample produced with the method has the advantages of high purity level, the industrialized production can be realized easily.

The invention relates to an application of an artemisinin compound in treatment of coronavirus infection. Specifically, the invention provides application of the compound, and a stereoisomer, a pharmaceutically acceptable salt, a solvate or a hydrate thereof in preparation of medicines. The medicines are used for treating diseases or infection caused by coronavirus (preferably SARS-CoV-2), and thecompound is selected from one or more of artemisinin, arteether, artemether, artemisinin, dihydroartemisinin, artesunate, artemisinin B and arteannuic acid.

The invention discloses a preparation method and application of artemisinic acid magnetic imprinting microspheres. Artemisinic acid serves as a template molecule, artemisinin serves as an auxiliary template, a magnetic Fe3O4 nano particle serves as a carrier, 4-vinylpyridine serves as a functional monomer, a TRIM-DVB mixture serves as a cross-linking agent, a surface imprinting technology is adopted, and the artemisinic acid magnetic imprinting microspheres are prepared; the obtained molecularly imprinting polymer particles are uniform in size, large in specific area and high in imprinting efficiency, the maximum static apparent adsorption amount of the imprinting microspheres for artemisinic acid is high, the artemisinic acid magnetic imprinting microspheres serve as adsorbent, enriched adsorbent can be selected from artemisia annua linn extract efficiently, the adsorbent can be rapidly separated through a magnet, washing and elution are conducted with solvent, a target compound can be separated, the recovery rate of artemisinic acid is 96.7%, and the product purity obtained after removal of solvent is higher than 80%. A novel separation material and method are provided for separation and purity of the bio active substance, namely artemisinic acid, in the artemisia annua linn.

The invention provides a method for producing artemisinic acid through fermentation. The method includes: adding a small amount of an organic solvent into a fermentation broth; transferring a product into an organic phase from the fermentation broth. The objectives of prolonging biosynthesis time, improving biosynthesis efficiency, increasing yield and simplifying extraction process are achieved. By the technical scheme, production procedures are reduced, and production efficiency is improved; the method is simple and easy to implement and suitable for industrialized production.

The invention discloses a construction method of gene engineered bacteria for arteannuic acid production, and the construction method comprises the following steps: A) construction of a gene expression module including an artemisinic acid production related gene, an upstream promoter of the artemisinic acid production related gene, and a downstream terminator of the artemisinic acid production related gene; and B) co-transformation of the gene expression module into saccharomyces cerevisiae. The method has the advantages of being simple and fast and efficient, avoids multiple cloning, and does not rely on enzyme digestion sites, many fragments can be together transformed, the homologous recombination efficiency is high, and the method can significantly shorten the construction time of the gene engineered bacteria.

The invention provides a method for increasing a fermentation yield of artemisinic acid. The method comprises the following steps: using an engineered strain of Saccharomyces cerevisiae capable of producing the artemisinic acid as a production strain; and fermenting in a fermentation culture medium containing a lactose hydrolysate capable of hydrolyzing into glucose and galactose, mevalonic acid and/or citral and other exogenous factors. The method provided by the invention increases the fermentation yield of artemisinic acid, reduces the fermentation cost of artemisinic acid, adopts a simpleand feasible technical solution, and has advantages of environmental protection, high genetic stability of the strain, and easy industrial production and application.

The invention relates to a method and equipment for preparing artemisinin by using residue obtained through extraction of artemisinin from sweet wormwood herb. The method comprises: 1) adopting an organic solvent to extract arteannuic acid and dihydroarteannuic acid from residue obtained through extraction of artemisinin from sweet wormwood herb; 2) adopting hydrazine hydrate to reduce the arteannuic acid obtained in the step 1) into a dihydroarteannuic acid crude product; 3) dissolving the dihydroarteannuic acid crude product obtained in the step 2) in an organic solvent, adding a photosensitizer and an acid catalyst, and adopting a light source to carry out a light oxidation reaction of the dihydroarteannuic acid so as to synthesize a artemisinin crude product; and 4) carrying out column chromatography and recrystallization purification on the artemisinin crude product obtained in the step 3). The invention further provides the equipment light oxidation reactor for performing the method. According to the present invention, the residue obtained through extraction of artemisinin from sweet wormwood herb is recycled so as to provide advantages of low environment pollution and resource saving; and the effective component in the residue obtained through extraction of artemisinin from sweet wormwood herb is completely utilized so as to increase the conversion rate of preparation of artemisinin from sweet wormwood herb.

The invention discloses a method for preparation of high purity arteannuic acid by middle and low pressure preparation chromatography, and the method comprises separation and purification of artemisinin and artemisinic acid, dry-method column mixing, middle and low pressure preparation chromatography column loading, elution, flow fraction collecting and other steps. The method provided by the invention is simple and practical, easy in operation, and fast in separation speed, can shorten the separation time, effectively improves labor efficiency, saves the production capital, and is beneficial to industrial production, and the prepared product is good in quality, high in purity, and the high purity of the artemisinic acid is up to above 98%.

The invention belongs to the technical field of medicines, and particularly relates to application of compounds arteannuic acid, artemisinin, chlorogenic acid, geniposide and galuteolin in preparationof an anti-A6 coxsackie virus medicine. The biological activity of the anti-A6 coxsackie virus medicine of arteannuic acid, artemisinin, chlorogenic acid, geniposide and galuteolin is found for the first time, the interaction between the anti-A6 coxsackie virus medicine and virus target protein is researched, and the anti-A6 coxsackie virus mechanism of the anti-A6 coxsackie virus medicine is disclosed.

The present invention relates to a method for separating high-purity artemisinic acid from a yeast cell fermentation liquid and purifying, particularly separating the artemisinic acid from the fermentation liquid and purifying by using an extraction-crystallization method. The method needs no column chromatography, the process is simple, the production conditions are easy to control and the method is suitable for large-scale industrial production. The method provided by the present invention crystallizes by adjusting the pH by taking water as a solvent, and is low in cost and environmental-friendly. Moreover, the artemisinic acid obtained by the method is high in purity.

The invention discloses a preparation method of artemisinin, wherein the preparation method comprise the steps: with artemisinic acid as a starting material, obtaining dihydroartemisinic acid under the hydrogen/metal catalyst action, then oxidizing dihydroartemisinic acid into arteannuic acid dihydrogen peroxide by hydrogen peroxide in the presence of sodium molybdate, and finally acting with oxygen under the catalysis of copper trifluoromethanesulfonate, to obtain the target product artemisinin with high yield. Compared with the prior art, the preparation method has the following advantages:the used reagents are cheap and easy to get, the synthetic route is short, the reaction selectivity is high, the preparation process is environmentally friendly, the operation and post-processing aresimple, the total yield is high, and the preparation method is suitable for industrialized production.

The invention relates to a sesquiterpene compound and an extraction and purification process thereof, in particular to a sesquiterpene effective part extracted from artemisia apiacea and an extractionprocess of the sesquiterpene effective part. The content of sesquiterpene in the artemisia apiacea sesquiterpene effective part is higher than 55%, and the artemisia apiacea sesquiterpene effective part mainly comprises the following compounds: 1: artemisinin B; 2: artemisinin; 3: artemisinin C, 4: artemisinic acid and 5: dihydroartemisinic acid. The sesquiterpene effective part can be used for preparing antimalarial drugs. The sesquiterpene effective part can also be used for preparing medicines with the effects of clearing deficiency heat, removing bone steaming, relieving summer heat, removing jaundice and the like. Meanwhile, the invention further provides a preparation method of the sesquiterpene effective part, and the method is simple, efficient and suitable for industrial application.

The invention discloses a method for preparing artemisinin for malaria. Firstly, dihydro-artemisinic acid is obtained by artemisinic acid under the effect of a reducing agent such as sodium borohydride /nickel chloride or hydrogen/a metal catalyst, then, under the existence of the catalyst, the dihydro-artemisinic is oxidized to be peroxide artemisinic acid through peroxide, and finally, an objective product-the artemisinin can be obtained with high efficiency under the catalyzing of the acid and the effect of oxygen. Compared with the prior art, the method for preparing the artemisinin for malaria has the advantages that the reagent is cheap and easy to obtain, short in synthetic route, high in reaction selectivity, environment-friendly in the process of preparation, easy to operate, simple in post-processing, high in total collection efficiency and suitable for industrialized production.

The invention relates to a pharmaceutical composition for preventing and treating avian salmonellosis and a preparation method of the pharmaceutical composition. The pharmaceutical composition comprises the following components: artemisinic acid, ampicillin sodium, ciprofloxacin and polysaccharide and probiotic microcapsules. According to the pharmaceutical composition disclosed by the invention, monomer traditional Chinese medicine effective components are selected to be combined with the antibiotics, so that the use amount of traditional Chinese medicines and antibiotics can be reduced on the premise of improving the antibacterial inhibition and killing effects, and the bioavailability and efficacy of medicines are improved. Meanwhile, immune enhancement regulation is taken as an auxiliary treatment means, polysaccharide and probiotics are selected to regulate intestinal flora of the diseased chickens and reconstruct a good microbial system, so that the immune function of the chickens is enhanced, and the production performance is improved. The pharmaceutical composition disclosed by the invention can be administrated through mixed feeding, is suitable for clinical application, has an exact curative effect and is suitable for being applied to intensive large-scale farms.

The invention provides a method for preparing artemisinin by adopting a continuous flow reactor, which comprises the following steps of: converting dihydroartemisinin serving as an initial raw material into MPDHAA in a continuous flow reactor in the presence of two metal catalysts (including sodium molybdate) and hydrogen peroxide, and then generating artemisinin from the MPDHAA in the continuous flow reactor in the presence of TFA (Trifluoroacetic Acid) and oxygen. Compared with a traditional kettle type reaction process, the method has the advantages that time and solvents are remarkably saved, and the production cost is reduced.