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Artemisia apiacea sesquiterpene effective part and extraction and purification process thereof

A technology for sesquiterpenes and effective parts, which is applied in the field of effective parts of sesquiterpenes and extraction techniques thereof, can solve problems such as not being retrieved, and achieve the effects of good effect, low cost and simple operation

Active Publication Date: 2020-12-11
苏州满元生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, no relevant literature has been found on the development of effective parts of sesquiterpenes using Artemisia annua as raw material

Method used

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  • Artemisia apiacea sesquiterpene effective part and extraction and purification process thereof
  • Artemisia apiacea sesquiterpene effective part and extraction and purification process thereof
  • Artemisia apiacea sesquiterpene effective part and extraction and purification process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1-quantitative analysis method

[0048] Preparation method of reference substance: Accurately weigh 0.01211g, 0.01023g, 0.01015g, 0.01023g, 0.01015g, Put 0.00803g and 0.00923g in a 5mL volumetric flask, add acetonitrile to dissolve and make the concave part of the liquid surface of the solution flush with the scale line to constant volume, respectively make stock solutions of reference substances, store them in a refrigerator at 4°C, and set aside.

[0049] The preparation method of the test sample: Take about 1.0 g of the original medicinal material of Artemisia annua, weigh it accurately, add methanol precisely for ultrasonic extraction, the ratio of solid to liquid is 1:40g / mL, the extraction time is 0.5h, and the extraction frequency is 1 time. Then filter with quantitative filter paper, take the filtrate and place it in a round-bottomed flask, and rotovap until the solvent is evaporated to dryness. Wash the residue in the round-bottom flask with a small...

Embodiment 2

[0058] Uniform sampling method Take 20 g of the raw medicinal material of Artemisia annua, add 10 times the volume (g / mL) of petroleum ether, and extract under reflux for 1 h. After extraction, filter with quantitative filter paper, concentrate the filtrate to dryness under reduced pressure, and calculate the paste rate. Add 80mL of methanol and sonicate for 30min, and filter with quantitative filter paper after sonication. Put the filtrate into a 100mL volumetric flask, and make to volume. Take 1mL in a 15mL ep tube, dry it with nitrogen, add 8mL of acetonitrile, sonicate for 15min, pass through a 0.22μm microporous membrane, and take the filtrate for sampling.

Embodiment 3

[0060] Uniform sampling method Take 20g of Artemisia annua raw medicinal material, add 10 times the volume (g / mL) of petroleum ether, and ultrasonically extract for 1h. After extraction, filter with quantitative filter paper, concentrate the filtrate to dryness under reduced pressure, and calculate the paste rate. Add 80mL of methanol and sonicate for 30min, and filter with quantitative filter paper after sonication. Put the filtrate into a 100mL volumetric flask, and make to volume. Take 1mL in a 15mL ep tube, dry it with nitrogen, add 8mL of acetonitrile, sonicate for 15min, pass through a 0.22μm microporous membrane, and take the filtrate for sampling.

[0061] Example 2 and Example 3 extraction results in different ways are shown in Table 2.

[0062] Table 2 Experimental data and measurement results of different extraction methods ( n=3)

[0063]

[0064]

[0065] The sesquiterpenoids of Artemisia annua were stable in hot petroleum ether, and the total extractio...

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Abstract

The invention relates to a sesquiterpene compound and an extraction and purification process thereof, in particular to a sesquiterpene effective part extracted from artemisia apiacea and an extractionprocess of the sesquiterpene effective part. The content of sesquiterpene in the artemisia apiacea sesquiterpene effective part is higher than 55%, and the artemisia apiacea sesquiterpene effective part mainly comprises the following compounds: 1: artemisinin B; 2: artemisinin; 3: artemisinin C, 4: artemisinic acid and 5: dihydroartemisinic acid. The sesquiterpene effective part can be used for preparing antimalarial drugs. The sesquiterpene effective part can also be used for preparing medicines with the effects of clearing deficiency heat, removing bone steaming, relieving summer heat, removing jaundice and the like. Meanwhile, the invention further provides a preparation method of the sesquiterpene effective part, and the method is simple, efficient and suitable for industrial application.

Description

technical field [0001] The invention relates to a sesquiterpene compound and an extraction and purification process thereof, in particular to an effective part of the sesquiterpene compound extracted from Artemisia annua and an extraction process thereof. Background technique [0002] The basis of the action of traditional Chinese medicine is the organic combination of various active ingredients, which act on multiple disease-related targets in the body through multiple channels, and exert an overall regulatory effect on the body. Artemisia annua is the dry aboveground part of Artemisia annua L., a plant in the Compositae family. It has the functions of clearing heat and relieving heat, removing steam, and cutting off malaria. Fever, malaria cold and fever, damp heat jaundice, etc. Due to the star effect of artemisinin, almost all the research on Artemisia annua is carried out around artemisinin, and there is very little research on the development of pharmaceutical product...

Claims

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Application Information

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IPC IPC(8): C07D493/10C07D493/20C07C57/26C07C51/42C07C51/47A61K31/365A61K31/366A61K31/19A61K36/282A61P33/06G01N30/02
CPCC07D493/10C07D493/20C07C57/26C07C51/42C07C51/47A61K36/282A61P33/06G01N30/02C07B2200/07C07C2602/28G01N2030/027Y02P20/54Y02A50/30
Inventor 王满元张梅
Owner 苏州满元生物科技有限公司
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