Method for preparation of high purity arteannuic acid by middle and low pressure preparation chromatography

A low-pressure preparative chromatography and artemisinic acid technology, applied in the field of preparing high-purity artemisinic acid, can solve the problems of difficult separation of artemisinic acid, many extraction impurities, large solvent consumption, etc., and achieves shortened separation time, high purity, and separation speed. quick effect

Inactive Publication Date: 2015-03-11
ANHUI SUNSHINE BIO TECH
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Problems solved by technology

At present, there are many research reports on the extraction and purification methods of artemisinic acid components of Artemisia annua, but the traditional extraction and separation process has the disadvantages of long separation time, many extraction impurities, low purity, and large solvent consumption, and it is not easy to separate and obtain high purity. High Purity Artemisinic Acid

Method used

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Embodiment

[0023] A method for preparing high-purity artemisinic acid by medium and low pressure preparation chromatography, comprising the following steps:

[0024] (1) Separation and purification of artemisinin and artemisinic acid

[0025] Take 500g of Artemisia annua extract and use 8 times the volume of petroleum ether to reflux extract three times at 60°C, then concentrate the extract until dry to obtain a crude extract; dissolve the obtained crude extract in 100ml of ethyl acetate, dissolve, and anhydrous Dry over sodium sulfate, filter;

[0026] (2) Dry column mixing

[0027] Add 100g of 400-mesh silica gel to the filtrate obtained in step (1), stir evenly, evaporate the silica gel at 60°C, and prepare for column loading;

[0028] (3) Medium and low pressure preparative chromatography column packing

[0029] The length of the column is 350mm, and the inner diameter is 40mm. First fill the new treated silica gel, then fill the silica gel in step (2) evenly a...

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Abstract

The invention discloses a method for preparation of high purity arteannuic acid by middle and low pressure preparation chromatography, and the method comprises separation and purification of artemisinin and artemisinic acid, dry-method column mixing, middle and low pressure preparation chromatography column loading, elution, flow fraction collecting and other steps. The method provided by the invention is simple and practical, easy in operation, and fast in separation speed, can shorten the separation time, effectively improves labor efficiency, saves the production capital, and is beneficial to industrial production, and the prepared product is good in quality, high in purity, and the high purity of the artemisinic acid is up to above 98%.

Description

technical field [0001] The invention relates to a method for preparing high-purity artemisinic acid by medium-low pressure preparation chromatography, which belongs to the technical field of medicine. Background technique [0002] Artemisia annua, also known as Artemisia annua, is an annual herb of the genus Artemisia in the Asteraceae family. It is widely distributed in various provinces in China and is a traditional Chinese herbal medicine. The main medicinal ingredients in Artemisia annua include artemisinic acid, artemisinin, artemisinin I, II, a-pinene, camphor, argentin, artemisinin, etc., and flavonoids. Artemisinic acid isolated from Artemisia annua has antimalarial, antibacterial, antiparasitic, instant heat effects, etc. It can treat various types of malaria. It has the advantages of quick action and low toxicity, especially for falciparum malaria and cerebral malaria. At present, there are many research reports on the extraction and purification methods of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 李冬冬代国栋吴金久李宏星王静静韦海阳蒋国强张天才
Owner ANHUI SUNSHINE BIO TECH
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