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Pharmaceutical composition for preventing and treating avian salmonellosis and preparation method thereof

A technology for fowl salmonella and a composition is applied in the field of veterinary medicine to achieve the effects of improving antibacterial and killing effects, improving drug sensitivity and enhancing bactericidal effect

Active Publication Date: 2021-11-02
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the combination of active ingredients of single traditional Chinese medicine and antibiotics for poultry salmonellosis can reduce the consumption of traditional Chinese medicine and antibiotics and improve the bioavailability and efficacy of the medicine on the premise of improving the efficacy of the medicine, which is an ideal research and development strategy. way

Method used

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  • Pharmaceutical composition for preventing and treating avian salmonellosis and preparation method thereof
  • Pharmaceutical composition for preventing and treating avian salmonellosis and preparation method thereof
  • Pharmaceutical composition for preventing and treating avian salmonellosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The MIC value of artemisinic acid, ampicillin sodium and ciprofloxacin, and the joint antibacterial FICI index determination

[0028] (1) Determination of the MIC value of the active ingredients of monomeric traditional Chinese medicine and antibiotics

[0029] According to the method steps recommended by the National Committee for Clinical Laboratory Standardization (NCCLS), the MIC value of artemisinic acid monomer was determined by using a 96-well plate micro broth dilution method. Using E-test, through the drug-containing susceptibility test strip (Liofilchems.r.l, Italy), read the reading scale at the intersection of the inhibition zone and the test strip, and determine the MIC of the antibiotics ampicillin sodium and ciprofloxacin on the test bacteria value. The antibiotic ciprofloxacin (Lot#C10532503, ≧98%) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd.; Ampicillin Sodium (Lot.No.826H031, ≧85%) was purchased from Beijing Suolaibao...

Embodiment 2

[0037] Example 2 Preparation of Tremella polysaccharide+Bacillus subtilis+Candida utilis microcapsules

[0038] In this experiment, Bacillus subtilis (bacillus subtilis, purchased from Beijing Beina Chuanglian Biotechnology Research Institute, as feed yeast, strain number BNCC223758) and Candida utilis (Candida utilis, purchased from China Industrial Microorganism Culture Preservation Management The center is feed yeast, strain number CICC1769) as the model strain, tremella polysaccharide (food grade, purity 30%, product batch number 20200312, Xi'an Senran Biological Engineering Co., Ltd.) as the auxiliary core material, sodium alginate-chitosan The microcapsule is used as a carrier to prepare the microecological preparation microcapsule.

[0039] (1) Inoculate the fresh culture of Bacillus subtilis into tryptone liquid culture medium, shake and cultivate at constant temperature for about 24 hours (30-35°C, 150r / min), and expand the culture to 1.2×10 9 CFU / mL is appropriate (...

Embodiment 3

[0044] The in vitro release test of embodiment 3 polysaccharide+probiotic microcapsule

[0045] (1) Determination of microcapsule swelling degree

[0046] The microcapsules are spherical, and after washing and drying with normal saline, the particle diameter ranges from 1.0 to 1.5 mm. After the microcapsules were soaked in 0.9% normal saline for 3 hours, the particle size basically did not change significantly; in the artificial simulated gastric juice, the particle size did not change significantly after soaking for 1 hour, but began to expand after 2 hours, and the average diameter of the microcapsules after soaking for 3 hours was 1.8mm , the expansion rate is 120%; and in the simulated artificial intestinal fluid, after soaking for 10 minutes, the microcapsules start to expand. After soaking for 1 hour, the average diameter of the microcapsules can reach 1.8mm, and the microcapsule expansion rate reaches 200%.

[0047] The results proved that the microcapsules made of chi...

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Abstract

The invention relates to a pharmaceutical composition for preventing and treating avian salmonellosis and a preparation method of the pharmaceutical composition. The pharmaceutical composition comprises the following components: artemisinic acid, ampicillin sodium, ciprofloxacin and polysaccharide and probiotic microcapsules. According to the pharmaceutical composition disclosed by the invention, monomer traditional Chinese medicine effective components are selected to be combined with the antibiotics, so that the use amount of traditional Chinese medicines and antibiotics can be reduced on the premise of improving the antibacterial inhibition and killing effects, and the bioavailability and efficacy of medicines are improved. Meanwhile, immune enhancement regulation is taken as an auxiliary treatment means, polysaccharide and probiotics are selected to regulate intestinal flora of the diseased chickens and reconstruct a good microbial system, so that the immune function of the chickens is enhanced, and the production performance is improved. The pharmaceutical composition disclosed by the invention can be administrated through mixed feeding, is suitable for clinical application, has an exact curative effect and is suitable for being applied to intensive large-scale farms.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and in particular relates to a pharmaceutical composition for preventing and treating poultry salmonellosis and a preparation method thereof. Background technique [0002] Avian salmonellosis is a type of bacterial disease caused by one or more Salmonella in the Enterobacteriaceae family that seriously threatens the intestinal health of poultry. According to the different antigenic structures of the pathogens, it can be divided into pullorum, fowl typhoid and avian paralysis. Three types of typhoid fever. Among them, the morbidity and mortality of pullorum are the highest, and it is popular. Chicks within 2 to 3 weeks of age are the most susceptible. After infection, chicks show diarrhea, white, pasty and thin feces, and the fluff around the anus is often covered with feces. It is polluted and forms lime-like hard lumps after drying; adult chickens are chronically or recessively infected. Alth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61K9/50A61K47/36A61P1/00A61P31/04A61K31/192A61K31/43A61K31/496A61K31/715A61K35/742
CPCA61K31/192A61K31/43A61K31/496A61K31/715A61K35/742A61K36/064A61K9/5036A61P1/00A61P31/04A61K2300/00
Inventor 王玲郭文柱沙赫巴兹郭志廷崔东安王彩玲魏小娟杨峰王春梅吕嘉文杨珍程峰
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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