65 results about "Salmonella gloucester" patented technology

The invention discloses a preparation and application of an oral tumor vaccine with attenuated salmonella typhimurium as a vector. The preparation of the vaccine comprises the following steps: (1) firstly, establishing a recombined vaccine vector integrating a micro gene with a self-transfer system through a molecular biological method; and (2) electrically transforming the recombined vaccine vector created in the step (1) into attenuated salmonella typhimurium SL7207 to establish the recombinant oral tumor vaccine. According to the preparation disclosed by the invention, the micro gene and the self-transfer system are integrated onto one vector for the first time, the salmonella typhimurium is electrically transformed to obtain the oral tumor vaccine, and the immune prevention effect of the vaccine on tumors is verified by a mouse tumor model, so that the oral tumor vaccine can be applied in the field of preparing immune preventive medicines for tumors.

The invention provides a salmonella detection primer group, a salmonella detection method and a salmonella detection kit based on an RPA-LbCas12a-TTECDS system, and relates to the field of salmonella detection. Based on the combination of a recombinase polymerase amplification technology and a CRISPR gene detection technology, salmonella is detected through an optimized and modified crRNA double-target gene combination, crRNA is screened and optimized aiming at an invA gene and a fimY gene of the salmonella respectively, and upstream and downstream primers for RPA reaction are designed according to nucleotide sequences at two ends of a matched sequence of the crRNA, so that the primer group is obtained. According to the primer group and the crRNA, the RPA-LbCas12a-TTECDS system is designed, and t hesalmonella detection method and kit are established according to the system. The detection method and the kit provided by the invention are used for detecting actual samples, and have the advantages of strong specificity, rapid detection, high sensitivity and high precision.

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention discloses specific methods such as raw material request, aqueous medium request, ozone water temperature and immersion time, etc. of a technical method for reducing survival percentage of salmonella on the surfaces of eggs by ozone water. The eggs to be processed in the invention are produced by healthy chicken group without salmonella disease and the eggs are fresh eggs with a guarantee period of no more than 7 days. The aqueous medium for preparing the ozone water meets the national NY5028-2001 water quality requirement for processing water of pollution-free food poultry product. When an ozonizer is used for preparing the ozone, the performance of the ozonizer can ensure the ozone concentration in the prepared ozone water to achieve above 4mg/L under the condition of 0 to 30 DEG C. The ozone concentration of ozone water which can reduce the survival percentage of the salmonella on the surfaces of the eggs by above 90 percent is higher than 2mg/L with the optimum temperature of 0 to 5 DEG C. Under the condition of 0 to 30 DEG C, the eggs are soaked in the ozone water with the ozone concentration of above 2mg/L for 10 minutes and then taken out; under the clean condition with the temperature of 20 plus or minus 5 DEG C and the relative humidity of below 30 to 50 percent, the eggs are treated with natural drying or air seasoning and packaged, and then the egg product, the survival percentage of the salmonella on the surface of which being reduced by above 90 percent, is obtained. The methods of the invention can be used for improving the safe mass of the eggs and have broad application value.

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

The invention relates to a product for preventing and/or treating salmonella infection, which belongs to the technical field of biological medicines. The product provided by the invention can inhibitthe growth of salmonella; the growth amount of salmonella is reduced by 90.4 percent; according to the product disclosed by the invention, the adhesion and invasion capabilities of salmonella can be reduced; the salmonella adhered to and invading HT-29 cells is obviously reduced; moreover, the product provided by the invention can inhibit the expression of salmonella virulence genes; the expression quantities of salmonella virulence genes InvA, AvrA, SsrB, HilA and SopD are respectively reduced by 69.3 percent, 62.4 percent, 57.4 percent, 53.4 percent and 31.3 percent; the inhibition effect isobviously superior to that of single use of ZS2058 or eugenol, so that the product provided by the invention has a strong effect of preventing and/or treating salmonella infection.

The invention discloses a novel coronavirus (SARS-CoV-2) vaccine antigen presentation system of attenuated salmonella secretory expression NTD structural domain protein and application of the novel coronavirus (SARS-CoV-2) vaccine antigen presentation system. The novel coronavirus (SARS-CoV-2) vaccine antigen presentation system is used for preventing novel coronavirus (SARS-CoV-2). By constructing the controllable and stable expression plasmid for secreting and expressing the NTD structural domain protein and performing engineering modification on the attenuated salmonella, the antigenic protein can be efficiently delivered to an antigen presenting cell by virtue of a specific secretion system of the attenuated salmonella after administration in an oral way. The delivered antigenic protein can be effectively treated and presented by antigen presenting cells, and finally, the immune system is activated/regulated, an antibody is generated, and the effect of a vaccine is achieved.

The invention discloses a primer for RPA rapid test of salmonella carrying a sulfonamides resistance gene and application of the primer. The primer has better interspecies specificity and intraspecies conservativeness, the technology can detect salmonella and sulfonamides resistance gene (sul3) in one reaction, whether the bacterium is the salmonella carrying the sulfonamides resistance gene (sul3) can be judged in short time, no false positive occurs, expensive instruments are not needed, and accordingly, the primer is more convenient, efficient and accurate. The segments after amplification are different is length and can be distinguished by electrophoresis.

The invention relates to a salmonella choleraesuis gene deletion mutant C7821 without a resistant marker, with collection number being CCTCCNO: M2010102. The invention also relates to a salmonella choleraesuis gene deletion vaccine prepared by the mutant. The gene engineering strain of the mutant is derived from the salmonella choleraesuis C78-2 strain (which is a commercial strain purchased from the China Veterinary Culture Collection Center of China Institute of Veterinary Drugs Control). The main virulence adjusting gene crp of the mutant deletes 320bp, so that the bacteria can not ingest cAMP from the mammals and grow more slowly, and the virulence of the bacteria is greatly reduced, thus the mutant has high safety. The animal tests prove that the gene deletion mutant strain has good protective immunity. The gene deletion vaccine prepared by the invention can stimulate the swine to generate protective immune response resistant to salmonella choleraesuis homologous wild strains and effectively prevent infection with salmonella choleraesuis.

The invention discloses salmonella phage SEE-1 and an application thereof, relates to prevention and control of salmonella, and belongs to the field of bioengineering. A novel bacteriophage SEE-1 is separated from a sewage treatment system, the preservation No. is CGMCC NO.1834D, the basic property of genomes is analyzed, and particularly, functional genes and protein are predicted and analyzed. The separated salmonella phage SEE-1 shows quick and efficient splitting efficiency in experiment of splitting the bacterium enteritidis, and can be used for preventing acterium enteritidis or drug resistant acterium enteritidis in the sewage treatment system.

The invention belongs to the technical field of animal infectious disease prevention and treatment, and particularly relates to a broad-spectrum high-temperature-resistant salmonella virulent bacteriophage and application thereof. The salmonella bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20211353. The salmonella bacteriophage has a good killing effect on salmonella drug-resistant strains, also has a good treatment effect on salmonella infection, is suitable for being used as a biological agent for preventing and controlling salmonella, and can be applied to comprehensive prevention and control of salmonella.

The invention relates to the technical field of detection, in particular, to salmonella specific forward and reverse primers and probe, a detection kit and an application of the detection kit. The detection kit includes the forward and reverse primers, the probe and a lateral flow chromatography test strip; the 5' end of the reverse primer is labeled by using biotin; and the 5' end of the probe islabeled by using fluorescein isothiocyanate (FITC), a C base in the middle region (at least 30 bp away from the 5' end and at least 15 bp away from the 3' end) is replaced by using [THF[, and the 3'end is end-capped by using C3-spacer. The detection kit can realize real-time on-site immediate and quick field detection by using the isothermal recombinase polymerase amplification and the lateral flow strip (RPA-LFS) method in the non-laboratory environment without relying on instrument equipment.

The invention relates to an application of sophora alopecuroide total alkali in preparation of a traditional Chinese medicine preparation for treating chicken pullorum as well as the traditional Chinese medicine preparation and a preparation method thereof. The traditional Chinese medicine preparation is used for treating salmonella infection of the chicken pullorum. The traditional Chinese medicine preparation consists of the sophora alopecuroide total alkali and auxiliary materials; and the preparation method comprises a step of preparing the sophora alopecuroide total alkali and auxiliary materials into powder or granules by virtue of a preparation process, and the prepared preparation is clinically used for treating the chicken pullorum by virtue of drinking water or stirring. The preparation provided by the invention does not have toxic or side effects and is simple in processing technology and low in production cost, and pharmacodynamics researches show that the preparation provided by the invention has the effects of preventing and treating salmonella of the chicken pullorum, and does not have antibiotic residues after being taken.

The invention discloses a primer, a kit and a detection method for detecting salmonella at a constant temperature on the basis of polymerase spiral reaction. The invention designs a pair of detectionprimers Ft/Bt and one pair of acceleration primers IF/IB by aiming at the specific region of the specificity target sequence invA conserved region of the salmonella, the nucleotide sequence of the primer is disclosed in SEQ ID NO. 1-4, and the reliability of a detection result is guaranteed by the primer. The invention also provides a kit for detecting the salmonella on the basis of the polymerasespiral reaction. The kit has the characteristics of high sensitivity, good specificity, convenience and high speed in operation, accurate and reliable result and low cost and is suitable for small and medium size departments and field detection application. In addition, the invention also provides a detection method for detecting salmonella at the constant temperature on the basis of the polymerase spiral reaction. According to the method, under a constant temperature condition, short time is consumed, an amplified product does not need gel electrophoresis, and a result can be directly judgedwith eyes after fluorescent dye is directly used for color development.

The invention discloses an anti-tumor composition based on a nano-silver material and application of the anti-tumor composition. The anti-tumor composition comprises a drug A and a drug B, wherein the drug A is attenuated salmonella, and the drug B is sialic acid modified nano-silver. The drug A and the drug B are combined for use and can be used for preparing medicines for treating tumors. Attenuated salmonella in the anti-tumor composition is planted in a tumor site in a targeted mode, sialic acid modified nano-silver is targeted to neutrophils, and efficient targeted delivery to the tumor site is achieved under the recruitment effect of salmonella; the nano-silver reaching the tumor site can be used for enhancing the curative effect of salmonella by directly killing tumor cells and removing neutrophils, and the biological safety of bacterial treatment is improved by removing salmonella. The anti-tumor composition can solve the key bottleneck problem of salmonella in clinical application, and efficient and safe tumor treatment based on salmonella is achieved.

The invention provides a method for detecting enteropathogenic bacteria (salmonella) through a seven-channel chip and application. The seven-channel chip includes a specific recombinase polymerase primer for detecting the enteropathogenic bacteria (salmonella), a probe and a seven-channel chip body, the sequence of a forward primer is SEQ ID No:1, the sequence of a reverse primer is SEQ ID No:2, the corresponding sequence of the probe is SEQ ID No:3, and the seven-channel chip takes a polymer as a material and is prepared through micro processing. The seven-channel chip includes seven orderedmicro channels which can distinguish multiple signals and prevents cross contamination between multiple systems. The method for detecting the enteropathogenic bacteria (salmonella) based on the micro-fluidic chip is provided to achieve fast, safe, specific and simple on-site detection of the salmonella. The probability of simultaneous detection of multiple samples is provided, and an effective scheme is provided for preventing the occurrence of salmonella epidemic situations and ensuring the food safety.

The invention relates to the field of food safety detection, and discloses primer pairs, a method and a kit for rapidly detecting salmonella through an RPA-LFD method and application. The primer pairs are InvA-F, InvA-R, Stm-F, Stm-R, Sen-F and Sen-R. The detection method comprises the steps of extracting total DNA of a sample, performing RPA reaction and parameter optimization by using the primer pairs, detecting by using a target lateral flow chromatography test strip, and judging whether the sample contains salmonella and serotypes such as mouse typhoid and enteritis or not according to the strip, wherein the whole detection process can be completed within 1 hour. The kit comprises sterile double distilled water, a buffer solution, standard positive DNA, a target lateral flow chromatography test strip and a product diluent. The primer pairs, the method and the kit for detecting the salmonella have the characteristics of being high in specificity, simple, convenient, rapid and the like, and can be used for rapid and accurate detection of the salmonella and the mouse typhoid and enteritis serotypes of the salmonella.

The invention relates to the technical field of pathogenic bacteria detection and discloses a quintuple PCR primer for detecting four salmonella serotypes, a kit and application thereof. The primer comprises five pairs of specific primers and is characterized in that the five pairs of specific primers are salmonella-F and salmonella-R; salmonella enteritidis-F and salmonella enteritidis-R; salmonella typhimurium-F and salmonella typhimurium-R; salmonella typhi-F and salmonella typhi-R; and salmonella suipestifer-F and salmonella suipestifer-R respectively. The quintuple PCR primer disclosed bythe invention not only can detect whether to be salmonella, but also can simply and accurately identify whether to be salmonella typhimurium, salmonella enteritidis, salmonella typhi and salmonella suipestifer at the same time through PCR detection without the aid of other auxiliary technical means.

The invention discloses a CPA detection primer, a detection kit and a detection method of salmonella. The CPA primer designed aiming at a target invA comprises stripping primers 4s and 5a, a cross amplification primer 2a1s and specific primers 2a and 3a; the nucleotide sequences of the primers are respectively as shown in SEQ ID NO.1 to SEQ ID NO.5. The cross primer isothermal amplification reaction detection and identification system designed for the salmonella specific target sequence invA provided by the invention overcomes the defects of long period, low sensitivity, high cost, difficulty in field application and the like of the method in the prior art. By selecting a conserved region of a specific sequence invA of a target strain, a pair of stripping primers, a cross primer and a specific primer are designed to construct a cross primer isothermal amplification reaction system, and a detection result can be obtained within about 60 minutes so as to shorten the traditional salmonella detection period.