27 results about "Salmonella gloucester" patented technology

The present invention relates to an orally administered Salmonella typhi Ty21a strain, optionally in combination with a checkpoint inhibitor, comprising a DNA molecule comprising at least one eukaryotic expression cassette encoding at least one tumor antigen, stromal antigen, and / or checkpoint inhibitor antigen, for use in the treatment of cancer in a human subject following treatment with an antibiotic.

The invention relates to the technical field of salmonella detection, in particular to a salmonella molecular detection method and application thereof, and the salmonella molecular detection method comprises the following steps: S1, sample treatment: extracting DNA in a to-be-detected sample; step S2, primer design and synthesis: specific primers are designed for the invA gene, the hilA gene and the stn gene of salmonella; s3, multiple PCR reaction: taking the DNA extracted in the step S1 as a template, and carrying out multiple PCR amplification by adopting the primer in the step S2; s4, fluorescent probe hybridization: hybridizing a PCR amplification product in the step S3 with a fluorescent labeled probe; s5, fluorescence signal detection: detecting a fluorescence signal after hybridization through a fluorescence detector, and judging whether the sample contains salmonella or not according to the signal. According to the salmonella molecular detection method provided by the invention, a technology of combining multiple PCR with fluorescent probe hybridization is adopted, a plurality of conserved genes of salmonella can be detected at the same time, and the detection specificity and sensitivity are improved.

The invention discloses a salmonella virulent bacteriophage vBSalPNW15 and application thereof, the bacteriophage is separated from farm sewage, belongs to a long-tail bacteriophage family, has a regular icosahedron head and a long-tail structure, and keeps stable under the conditions that the pH is 4-13 and the temperature is 40-60 DEG C. Genomic analysis shows that the kit does not contain virulence genes and drug-resistant genes and is good in safety. The bacteriophage and cinnamyl aldehyde are combined to be used for preventing and treating salmonella infection, both the bacteriophage and cinnamyl aldehyde show a remarkable synergistic bacteriostatic effect (FICI = 0.5) in vitro and in vivo, and the bacteriophage and cinnamyl aldehyde can effectively inhibit growth of multi-drug-resistant salmonella, remove biological membranes, relieve tissue pathological damage and remarkably improve the survival rate of chicks. The invention provides a safe and efficient antibiotic replacement scheme for preventing and treating salmonella infection, and is suitable for the fields of livestock and poultry breeding and food safety.

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The application discloses a kind of attenuated salmonella load adjuvant nano combination medicine, the combination medicine is modified to bacterial surface by means of amino and carboxyl condensation reaction, adamantane is simultaneously modified to adjuvant nano medicine surface using diselenium dynamic bond, then the host-guest interaction of adamantane and cyclodextrin is used to assemble two, and then nano medicine is stably combined on the surface of attenuated salmonella.Diselenium dynamic bond can be broken by ultrasound response, so as to realize the effect of drug release.This combination medicine has the tumor targeting enrichment ability of bacteria and the release characteristics of adjuvant nanoparticles under the action of ultrasonic wave, can realize targeted delivery and deep penetration in tumor tissue, and obtain significant tumor inhibition effect under ultrasonic stimulation.The experimental results show that the combination medicine exhibits good therapeutic effect in inhibiting tumor growth, and has excellent biological safety.

The utility model provides a kit for rapidly identifying and culturing drug sensitivity of salmonella, and relates to the field of salmonella culture. The salmonella rapid identification culture drug sensitivity kit comprises a kit body and a kit cover, a container used for culturing salmonella is fixedly installed in the kit body, a base plate is fixedly installed on the inner bottom wall of the container, a telescopic rod used for rotating is fixedly connected to the base plate, a filter screen and an arc-shaped plate are fixedly installed at the movable end of the telescopic rod, and the arc-shaped plate is fixedly connected with the filter screen. The filter screen is slidably connected with the container; the rapid identification and culture drug sensitivity kit for salmonella integrates sample separation, dynamic culture and drug sensitivity test, after culture is completed, a drug sensitivity reagent can be directly added for the drug sensitivity test, sample transfer is not needed, operation steps and time are reduced, rapid identification and drug sensitivity detection of salmonella are achieved, and the kit is suitable for large-scale popularization and application. And the detection efficiency and accuracy are improved.

The invention discloses a salmonella bacteriophage and antibiotic combined medication mode with a synergistic effect. The bacteriophage is salmonella bacteriophage SP4, and the antibiotic is cefotaxime sodium. The two bactericidal drugs are used for treating the chicken salmonellosis, and a model is established to verify the combination effect. Compared with an attacking control group, the application mode of the combination of the bacteriophage and the cefotaxime sodium disclosed by the invention can remarkably improve the growth performance of chickens and obviously reduce the content and death rate of salmonella in chicken manure, is a novel and creative application mode, can be used as a novel application strategy for treating chicken salmonellosis, and has a wide application prospect. Good market application values are realized.

The invention discloses a salmonella bacteriophage, a bacteriophage conjugate and a preparation method and application of the salmonella bacteriophage and the bacteriophage conjugate, and relates to the technical field of biology, the salmonella bacteriophage is bacteriophage 72-1B, the preservation number is M20252570, and the preservation unit is China Center for Type Culture Collection. The preparation method comprises the following steps: specifically coupling a fluorescent material with a bacteriophage 72-1B to prepare a bacteriophage 72-1B-fluorescent material 9-(2-bromoethyl) carbazole conjugate; the phage conjugate combines the characteristics of a fluorescent material and the phage and can specifically recognize and combine salmonella, and the detection limit is as low as 10 < 2 > CFU / mL; the distribution positions (such as food pollution sites and wound infection areas) of bacteria can be directly observed through a fluorescence microscope and flow cytometry, so that visual positioning is realized, an intuitive basis is provided for pollution traceability and targeted therapy, and blind prevention and control are avoided.

The present invention relates to an immunogenic composition comprising antigens from Salmonella enterica mouse typhoid serotype (Salmonella typhoid), Salmonella enterica enteritis serotype (Salmonella enteritidis), and Salmonella enterica typhoid serotype (Salmonella typhoid). The invention also relates to methods and uses of compositions comprising GMMA for enhancing an immune response against Salmonella typhi antigens, vaccines comprising said immunogenic compositions, and methods and uses of said immunogenic compositions.

The application discloses a method and application of sodium propionate for inhibiting Salmonella virulence factors, and comprises the following steps: inoculating Salmonella liquid into modified Martin culture medium for culture to obtain first bacterial liquid; inoculating the first bacterial liquid into new modified Martin culture medium for culture to obtain second bacterial liquid; centrifuging the second bacterial liquid, washing, and diluting by using modified Martin solution to obtain bacterial suspension; culturing the bacterial suspension and sodium propionate under certain conditions to obtain third bacterial liquid; and detecting the expression of virulence factors of Salmonella in the third bacterial liquid. It is found through detection that different concentrations of sodium propionate can significantly reduce the expression of Salmonella virulence factors. The method provided by the application can weaken the infection toxicity of Salmonella, so that the infection and incidence probability of poultry Salmonella can be reduced, and the infection symptoms can be alleviated.

The present invention relates to a vaccine composition comprising a live attenuated Salmonella Typhimurium bacterium intended for use in a method for reducing the severity of symptoms associated with Salmonella Choleraesuis infection and / or preventing Salmonella Choleraesuis infection in a pig, wherein the live attenuated Salmonella Typhimurium bacterium is the sole active ingredient of the vaccine composition, and wherein the method for attenuating the severity of symptoms associated with Salmonella Choleraesuis infection and / or preventing Salmonella Choleraesuis infection in a pig consists of administering said vaccine composition.

This invention relates to a fusion protein for preventing Salmonella infection, an immunogenic composition, a recombinant vaccine, and its molecular architecture design and applications. The invention selected three fusion proteins—PstS-LpfB, PstS-YidR, and LpfB-SinH—that can be successfully expressed in eukaryotic cells to design animal immunization experiments and verify their protective effects. This invention found that fusion protein molecules such as PstS-LpfB can attenuate tissue lesions caused by Salmonella infection, possess good immunogenicity, and play an effective preventive and immunoprotective role, highly preventing Salmonella infection and showing broad application prospects.

Methods of reducing or preventing intrauterine transmission of Salmonella Dublin in a subject, typically a cow, are provided. The methods include a first administration, and optionally second or more administrations, to a pregnant cow of an effective amount of an immunogenic composition to induce an immune response against S. Dublin in the cow. In some forms, the cow is infected with S. Dublin. In some forms, two or more cows are treated in parallel, for example, all cows that inseminated or otherwise became pregnant in parallel. Typically, the first administration, and optionally second or more administrations, are administered to the cow at a time(s) that reduces intrauterine transmission of S. Dublin infection from the cow to one or more of her calves compared to unvaccinated control cows and / or cows administered according to a different administration regimen. Exemplary methods of administration are provided.

The invention discloses a primer, a probe and a detection method for salmonella RAA fluorescent rapid detection and application. The technical problem to be solved is to provide a salmonella detection method which is based on an RAA technology and is high in sensitivity, strong in specificity and good in repeatability. In particular discloses a composition for detecting salmonella in a tested sample, the composition comprises a pair of SA-invA-F2 and SA-invA-R1 and a probe of SA-invA-Probe, the SA-invA-F2 is a single-stranded DNA molecule of which the nucleotide sequence is SEQ ID No.4, and the SA-invA-R1 is a single-stranded DNA molecule of which the nucleotide sequence is SEQ ID No.3; a structure of the SA-invA-Probe is shown as SEQ ID No.9-tetrahydrofuran residue-SEQ ID No.10, a 26th nucleotide T of the SEQ ID No.9 is used for marking a fluorophore, a first nucleotide T of the SEQ ID No.10 is used for marking a quenching group, blocking modification is carried out on a 3'terminal, the lowest detection limit can reach 276copies / reaction and 164CFU / reaction, and the SA-invA-Probe can be used for industrial production.

The present disclosure provides compositions and methods for making and using Salmonella recombinant bacteria that express LPS O78 antigen and / or virulence factors of avian pathogenic Escherichia coli (APEC) as vaccines to prevent colibacillosis infections.

This invention discloses a method for detecting Salmonella based on HCR-mediated multivalent aptamers and the CRISPR-Cas12a system. The method includes the following steps: 1) mixing streptavidin nanobeads with biotinylated aptamers to obtain aptamer magnetic beads; 2) mixing trigger strands, hairpin DNA H1, and hairpin DNA H2 to obtain an HCR scaffold, followed by the addition of aptamers to obtain an HCR-based multivalent aptamer structure. The detection method involves isolating and enriching Salmonella using aptamer magnetic beads, adding the HCR-based multivalent aptamer to obtain a magnetic bead-Salmonella-HCR sandwich structure, and then adding it to the CRISPR-Cas12a system. After reacting at 37°C for 1 hour, an enhanced fluorescence signal is obtained, enabling the detection of Salmonella in the test solution. The advantages are high sensitivity, strong specificity, and good accuracy.

The present application relates to the technical field of microorganism, and particularly relates to a streptococcus gallolyticus and application of the streptococcus gallolyticus in preparation of anti-salmonella infection preparation. Streptococcus gallolyticus The streptococcus gallolyticus can improve the resistance of a host to salmonella infection, and can be used for preparing probiotic preparation, functional feed additive or related health care product for preventing or assisting in treating salmonella infection.

Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.

Two stage heated water treatment of shell eggs results in-shell poached eggs. The in-shell poached are sufficiently cooked so when the eggs are cracked onto a plate the eggs have the characteristics of conventional poached eggs without further cooking and need only to be warmed for consumption. The in-shell poached eggs are also pasteurized in the shell to meet the FDA and WHO requirement of a 5-log reduction of Salmonella Enteritidis.

This invention relates to the field of genetic engineering technology, and in particular to the application of a deacetylated NDP52 protein in the preparation of drugs for controlling Salmonella infection. This application provides a deacetylated NDP52 protein, a gene encoding the deacetylated NDP52 protein and its nucleotide sequence, and includes a recombinant vector containing the gene, a transgenic cell line or recombinant virus, and a substance capable of deacetylation of the NDP52 protein. The application of the gene, the protein, and the substance for deacetylation of the NDP52 protein in the preparation of drugs for controlling Salmonella infection demonstrates that expressing NDP52 and its deacetylated variants in mouse liver effectively promotes the clearance of bacterial infection in the liver and reduces liver necrosis, providing a scientific basis and potential tool for the treatment of bacterial infectious diseases.

The application relates to the technical field of molecular biology, in particular to an anti-enteritis Salmonella nanobody Nb122, application and preparation method thereof, and relates to the nucleotide sequence and amino acid sequence of the anti-enteritis Salmonella nanobody Nb122, and relates to the application of the anti-enteritis Salmonella nanobody Nb122 in preparing products for reducing the adhesion and invasion of enteritis Salmonella to IPEC-J2 cells, and relates to the preparation method of the anti-enteritis Salmonella nanobody Nb122. The anti-enteritis Salmonella nanobody provided in the technical scheme can reduce the adhesion and invasion of enteritis Salmonella to IPEC-J2 cells.

The application relates to a positioning mechanism for detecting a Salmonella abortus equi live vaccine and a use method thereof, and belongs to the technical field of vaccine detection. The positioning component is provided, the positioning part in the positioning component can position the vaccine bottle, the vaccine bottle can be directly placed in the positioning part during use, alignment is not needed, the detection efficiency is improved, and the positioning mechanism is convenient to use. The positioning mechanism is provided, the positioning mechanism can meet the use requirement of manually placing the vaccine bottle through cooperation of the positioning part and the adjusting part, the carrying part is separated from the positioning support, the positioning part is separated from the adjusting part, the positioning support is loaded on the adjusting part, the positioning part is separated and loaded on the two positioning supports, the switching of the mechanism form can be completed, the adjusting part and the positioning support are matched with each other, the positioning part can be driven to expand or shrink, the vaccine bottle conveyed through the conveying belt can be positioned, and the functionality of the mechanism is improved.

This invention discloses a *Bdellovibrio* strain and its application in the treatment of *Salmonella lysate*, belonging to the field of microbial technology. The strain, with accession number CCTCC NO:M 20232311, has been identified as a new species of the genus *Bdellovibrio* through multiphasic classification, including 16S rRNA gene analysis, average nucleotide similarity, digital DNA-DNA hybridization, and trapping diet. This invention also discloses a biocontrol agent containing this *Bdellovibrio* and its application in the treatment of *Salmonella lysate*. The *Bdellovibrio* strain HCB006 provided by this invention exhibits high lysis efficiency and strong environmental adaptability, and can be used as an antibiotic alternative for the biocontrol of *Salmonella* in food processing, aquaculture environments, and medical fields. It is of great significance for preventing the spread of dangerous pathogens and improving food health.

The invention relates to the technical field of biomedicine detection, and discloses a biomedicine-based salmonella detection instrument and method for livestock and poultry breeding, the biomedicine-based salmonella detection instrument comprises a detection table and an electron microscope mounted at the upper end of the middle part of the detection table, a conveying assembly is arranged at the upper end of the detection table, and the conveying assembly is used for continuously feeding a culture dish; and the conveying assembly comprises two groups of rotating rods connected with the detection table. According to the salmonella detection instrument and method for livestock and poultry breeding based on biomedicine, stable power is provided for the conveying assembly through the first motor, and continuous and automatic conveying of culture dishes is achieved in cooperation with transmission of a synchronous belt wheel and a feeding belt; the feeding assembly synchronously completes accurate feeding of single culture dishes through the power of the conveying assembly by means of linkage of a driving belt wheel, a driven belt wheel and a transmission belt, a power source does not need to be additionally arranged, the structure is simplified, and meanwhile cooperation and consistency of the feeding rhythm and the conveying rhythm are achieved; and the detection requirements of large-batch salmonella in large-scale livestock and poultry breeding are effectively met.

The invention provides double probes for detecting salmonella, a kit as well as a preparation method and application of the kit, and belongs to the technical field of bacterial detection. The double probes comprise a capture probe and a detection probe; the capture probe is a magnetic bead coupled with a salmonella aptamer and a salmonella bacteriophage, and the detection probe is gold nanoparticles modified with fDNA; the salmonella aptamer and the fDNA are of a single-chain structure with mutually paired sequences. The double probes provided by the invention respectively promote the sensitive and convenient development of a salmonella detection technology through efficient capture, separation and enrichment and visual signal output. The scheme provided by the invention is simple to operate, low in cost, less in time consumption, easy to implement, high in sensitivity and high in accuracy, does not need large experimental equipment, and can realize visualization of detection results.

The invention relates to a VBNC salmonella enteritidis resuscitation culture medium and application, and belongs to the technical field of microorganism culture detection. The VBNC salmonella enteritidis resuscitation culture medium comprises 8 g / L-12 g / L of peptone, 3 g / L-7 g / L of sodium chloride, 7 g / L-11 g / L of disodium hydrogen phosphate, 1 g / L-2 g / L of monopotassium phosphate and 10 U / mL-1000 U / mL of Rpf recombinant protein. Experiments prove that the culture medium can detect VBNC salmonella enteritidis which cannot be detected by a traditional culture medium, and the VBNC salmonella enteritidis resuscitation culture medium is excellent in performance and high in detection rate.

The invention provides a double probe for detecting salmonella as well as a preparation method and application of the double probe, and belongs to the technical field of bacterial detection. The double probes provided by the invention comprise an aptamer-phage-magnetic bead probe MB (at) Phage (at) Apt and an fDNA-gold nanoparticle probe AuNPs (at) fDNA; the sequence of the aptamer is as shown in SEQ ID NO: 1, and the sequence of the fDNA is as shown in SEQ ID NO: 2. The double probes are assisted by an ultraviolet lamp, on-site rapid visual detection of salmonella is realized through system color change caused by change of fluorescence intensity, the provided detection method is simple to operate, low in cost, less in time consumption and easy to realize, and on-site rapid visual detection of salmonella can be realized without large experimental equipment; a data analysis system and a linear equation are further utilized for calculation, the content of salmonella can be detected in a short time, and the detection efficiency and accuracy are improved.