52 results about "Murine tumor" patented technology

The invention belongs to the field of cell sorting, in particular to a method for separating and purifying mouse tumor tissue infiltrating CD4 + CD25 + Methods for isolation and purification of regulatory T cells. It specifically includes the following steps: (1) Preparation of single-cell suspension of tumor tissue: Weigh 0.2 g of tumor tissue, cut it into small pieces, put it into a tissue grinder, add 5 mL of tissue homogenate to homogenize at 0°C for 2 min, pass through 200 mesh Filter through a filter, collect the filtrate, centrifuge in a vertical centrifuge at 20°C for 15 minutes at 250 g, discard the supernatant and save the precipitate, resuspend the precipitate with 2 mL of 80% fetal bovine serum sample diluent to form a single-cell suspension, and use the sample diluent to adjust the count The concentration is 1×10 8 cells / mL; (2) Separation of mouse tumor tissue infiltrating lymphocytes; (3) CD4 + CD25 + Immunomagnetic bead sorting of regulatory T cells, the collected cells are CD4 + CD25 + Treg. The method of the present invention uses tumor tissue for the first time to prepare a single cell suspension, and the finally obtained CD4 + CD25 + The purity of Treg reached 86.5%.

The invention discloses a <99m>Tc(HYNICPBB)(tricine / TPPTS) complex as well as a preparation method and an application. The <99m>Tc(HYNICPBB)(tricine / TPPTS) complex is obtained through two steps including synthesis of a ligand HYNICPBB and preparation of <99m>Tc(HYNICPBB)(tricine / TPPTS). The complex is simple to prepare, high in radiochemical purity and good in stability, has better water solubility and has certain uptake in MCF-7 cells, the uptake can be significantly inhibited by palbociclib, which indicates that the complex has a CDK4 / 6 specificity. The complex has higher uptake and retention and better tumor / muscle ratios at tumor parts of female Balb / c nude mice carrying MCF-7 tumor, has obviously reduced uptake of non-target organs such as liver and can be applied to preparation of anovel CDK4 / 6 targeting tumor developer.

The invention provides a humanized mouse tumor model, which is characterized in that the humanized mouse tumor model is obtained by transplanting human thymus tissue and CD34+ hematopoietic stem cells homologous to the thymus tissue. The present invention constructs a humanized mouse tumor model transplanted from thymus and hematopoietic stem cells for the first time. The humanized mouse tumor model has confirmed that it has a complete immunosuppressive microenvironment, and is suitable for the overall evaluation of solid tumor immunotherapy, and can be used to evaluate the feasibility and effectiveness of immunotherapy. The invention makes up for the singleness and incompleteness of the existing tumor immunotherapy evaluation methods, provides reference value for clinical research, and complements the key role of the human immune system in immunotherapy compared with traditional models.

Disclosed are monoclonal antibodies that bind specifically to macrophage stimulating protein receptor (or RON—Recepteur d' Origine Nantais). Also provided are the chimeric antigen receptors, bispecific antibodies, bivalent antibodies and biTE thereof, as well as pharmaceutical compositions and uses of said antibodies for the treatment of cancer and fibrosis and an ex vivo method of evaluating the status of a cancer patient using said antibodies. In particular, two monoclonal antibodies, 7G8 and 6D4, demonstrating good therapeutic efficacy in inhibiting tumour growth in human xenograft mice models and sensitivity in human xenograft mouse tumour imaging models are provided.

The invention relates to an antitumor traditional Chinese medicine composition and applications thereof, wherein the traditional Chinese medicine composition is prepared from the following bulk drugsby weight: 9-15 parts of Rhizoma Sparganii, 9-15 parts of Curcuma zedoaria, 15-20 parts of processed Pinellia ternata, 15-20 parts of processed Arisaema heterophyllum Blume, 15-30 parts of Milkvetch Root, and 15-30 parts of Atractylodes macrocephala. According to the present invention, the traditional Chinese medicine composition has effects of blood stasis removing, abdominal mass eliminating, phlegm removing, hard mass softening, body resistance supporting, spleen strengthening, qi benefiting and the like; the animal experiment results show that the traditional Chinese medicine composition can significantly inhibit the growth of MKN-45 gastric adenocarcinoma transplanted tumor in nude mice, down-regulate the PCNA expression and the EGFR expression in the gastric cancer tissue of nude mice, change the tumor microenvironment of walker-256 ascites model rats, and provide good treatment effects on tumors; and the traditional Chinese medicine composition has advantages of less variety ofbulk drugs, easy preparation, abundant and easily available raw materials and low cost.