51results about How to "Good blot" patented technology

The present invention relates to a porous imprinting carbon nano-sphere preparation method, which comprises adopting a glucose solution to carry out hydrothermal synthesis of carbon nano-spheres, and carrying out surface modification, sintering carbonization, and grafting modification on the porous carbon nano-spheres to finally prepare the porous imprinting carbon nano-spheres. The preparation method has characteristics of rigorous process, advancement, reasonability, and accurate and detailed data. The product has the following characteristics that: a morphology is good, the product is black round particles, a particle diameter is less than or equal to 180 nm, a porous imprinting effect is significant, the product can be matched with a plurality of chemical substances, product purity is good and achieves 99%, and a yield is high and achieves 91%. In addition, the preparation method is an ideal porous imprinting carbon nano-sphere preparation method.

The invention belongs to the technical field of functional material preparation, and particularly relates to a preparation method of a molecularly imprinted composite membrane for selectively separating tetracycline. The preparation method comprises the following steps: preparing a basement membrane from cellulose acetate and chitosan; preparing the tetracycline molecularly imprinted composite membrane by taking biomass nano activated carbon as a doping material, tetracycline as a template, acrylamide and methacrylic acid as functional monomers, ethylene glycol dimethacrylate as a cross-linking agent and azodiisobutyronitrile as an initiator and combining nano material doping technology and molecularly imprinted polymerization technology. Based on a biomass material synthesis strategy andin combination with the doping of biomass nano activated carbon and the synergistic effect of a bifunctional monomer, the prepared material has the advantages of high flux and strong selective adsorption capacity, so that the separation efficiency of the material on tetracycline in a complex mixed system is greatly improved; in addition, since the base membrane raw material of the molecularly imprinted membrane is a biomass and renewable material, the molecularly imprinted membrane has good biodegradability.

The invention provides molecularly imprinted polymeric microspheres for phenol. The polymeric monomer of the polymeric microspheres is methacrylic acid or 4-vinylpyridine and the specific surface area of the polymeric microspheres is 23.08-62.33m<2>/g. The invention also provides a method for preparing the polymeric microspheres and the use of the polymeric microspheres in collection or detection of phenol compounds. In addition, the invention also provides a chromatographic column and a solid phase extraction cartridge filler. The filler is the molecularly imprinted polymeric microspheres. The molecularly imprinted polymeric microspheres are simple and convenient in preparation, have an adsorption selectivity of 8.25 to 44.55 mg/g, reach a balance within 6 hours and have the advantages of high absorption selectivity and high absorption speed.

The invention relates to a method for preparing a molecularly-imprinted monolithic column by using a molecular crowding reagent and ionic liquid as a pore-foaming agent. The method comprises the following steps: through special formulation of the pore-foaming agent, adding a template molecule ursolic acid or oleanolic acid into a pore-foaming agent consisting of a macromolecular crowding reagent, the ionic liquid and dimethyl sulfoxide, adding a functional monomer, a cross-linking agent and an initiator, performing ultrasonic degassing, and introducing nitrogen for removing oxygen to obtain an ursolic acid or oleanolic acidmolecularly-imprinted monolithic column. By the method, the macromolecular reagent and the green solvent ionic liquid can form the molecularly-imprinted monolithic column with good permeability and a significant imprinting effect; the imprinting effect of the obtained ursolic acid or oleanolic acidmolecularly-imprinted monolithic column is better than that when the macromolecular reagent and the ionic liquid are independently used as the pore-foaming agent; the method has the characteristics of simple preparation, avoidance of a complicated preparation process, good molecular recognition performance and the like; the obtained molecularly-imprinted monolithic column has good permeability and a significant imprinting effect; a new method is provided for preparing the molecularly-imprinted monolithic column with better imprinting effect.

The invention discloses a preparation method of an adsorbent capable of reducing nicotine content in cigarette smoke. The preparation method comprises the following steps: performing molecular imprinting by a sacrifice silica gel skeleton method, after imprinting, removing silica gel by using hydrofluoric acid to solute, and obtaining microspheres with a molecular recognition function. The microspheres basically keep the shapes of primary silica gel microspheres, and have greater hole volumes and specific surface areas, a plurality of reaction sites and higher selectivity and adsorption capacity. The absorbent is applied to a composite rod, the addition amount can be regulated as required, when the addition amount is 10 mg/cigarette, compared with a comparing sample, the reduction rate of nicotine in cigarette smoke is 23.8%, and the relative reduction rate relative to tar is 10.4%; and when the addition amount is 20 mg/cigarette, compared with a comparing sample, the reduction rate of nicotine in cigarette smoke is 43.7%, and the relative reduction rate relative to tar is 23.4%; thus the effect of selectively reducing nicotine is very obvious.

The invention relates to preparation of a carbon nanotube doped fenbufen molecularly imprinted polymer sustained-release material and specifically relates to a fenbufen sustained-release molecularly imprinted polymer material which is prepared by utilizing ionic liquid 1-butyl-3-methylimidazole tetrafluoroborate and a deep-eutectic solvent as a binary pore-foaming agent and doping single-wall carbon nanotubes. The fenbufen sustained-release molecularly imprinted polymer material is prepared from raw materials by mass: 0.67 to 0.76% of fenbufen, 0.03 to 0.06% of single-wall carbon nanotube, 2.41 to 2.75% of 4-vinyl pyridine, 18.16 to 20.69% of ethylene glycol dimethacrylate, 18.37 to 48.39% of deep-eutectic solvent, 30.06 to 57.08% of 1-butyl-3-methylimidazole tetrafluoroborate and 0.25 to 0.29% of azodiisobutyronitrile. The fenbufen sustained-release molecularly imprinted polymer material disclosed by the invention has a good sustained-release effect; in-vivo pharmacokinetics show that compared with 1.5h peak time and 46.1% bioavailability of an imprinted polymer without carbon nanotubes, peak time of blood drug concentration in a rat body can reach 4 hours and bioavailability can be 140% compared with commodity medicine, and performance is stable. The preparation of the carbon nanotube doped fenbufen molecularly imprinted polymer sustained-release material disclosed by the invention lays a foundation for a molecularly imprinted technology to be applied to a medicine transfer system.

The invention relates to the technical field of solid phase extraction, and particularly discloses a prometryn molecular imprinting solid phase extraction column and an application method thereof. With prometryn as a template, methacrylic acid as a functional monomer, glycol dimethyl methacrylate as a cross-linking agent, azodiisobutyronitrile as an initiating agent, dichloromethane as a solvent, a precipitation polymerization method is adopted to prepare prometryn molecular imprintingpolymeric microsphere; microballoons are uniformly filled in the solid phase extraction column, thus obtaining the prometryn molecular imprinting solid phase extraction column; dichloromethane is utilized as, residuals of simazine, atrazine, szimetrin, propazine, ametryn, terbuthylazine, prometryn and terbutryn in a grain sample are simultaneously enriched and purified by taking a sample solvent and dichloromethane-acetonitrile as a leaching solvent and taking methyl alcohol-water as an eluting solvent, when used together with a capillary electrophoresis multiresidue detection method, the prometryn molecular imprinting solid phase extraction column can simultaneously detect the residuals of the eight herbicideds.

The invention discloses a method for synthesizing a clenbuterol hydrochloride molecularly imprinted polymer. The method comprises the following step: by using clenbuterol hydrochloride as a template molecule and alpha-methacrylic acid as a functional monomer and performing interaction in the form of hydrogen bonds, adding a crosslinking agent N,N-methylene-bis acrylamide and an initiator ammonium persulfate to perform free radical copolymerization, thereby obtaining the clenbuterol hydrochloride molecularly imprinted polymer, wherein the mole ratio of the template molecule, functional monomer and crosslinking agent is (117-274):(1-300):(30-90). The clenbuterol hydrochloride molecularly imprinted polymer has the advantages of simple and quick synthesis process, low cost, high specificity and favorable stability, can be combined with a test paper method to prepare a test paper strip, and is used for trace on-line detection of clenbuterol hydrochloride.

The invention belongs to the technical field of heavy metal ion adsorption separation and relates to an application and preparation method of a hexavalent chromium ion imprinted composite membrane with alpha-methacrylic acid serving as a functional monomer. The method includes that by adoption of hexavalent chromium ions as template ions, alpha-methacrylic acid as the functional monomer, glycol dimethyl acrylate as a cross-linking agent, azodiisobutyronitrile as an initiator and a pore forming solvent formed by mixed solution of an organic solvent and water in a volume ratio of 1:1, the hexavalent chromium ion imprinted composite membrane is prepared according to a surface grafting method. By combination of the ion imprinting technique and the membrane separation technique, complex operations such as grinding or screening are avoided, specific recognition sites can be protected, high stability, high environmental adaptability and availability for extreme solution environments are realized, and application to adsorption separation of hexavalent chromium ions in solution containing Cr (VI) is realized.

The invention provides a 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The invention also provides a method for preparing the 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The invention also provides application of the 2, 4, 6-trichlorophenol molecular engram microsphere polymer in enriching and/or separating 2, 4, 6-trichlorophenol. The invention also provides the application of the 2, 4, 6-trichlorophenol molecular engram microsphere polymer in monitoring and/or processing the 2, 4, 6-trichlorophenol in an environmental water sample. The invention also provides a method for detecting and/or processing the 2, 4, 6-trichlorophenol in a sample through the 2, 4, 6-trichlorophenol molecular engram microsphere polymer. The polymer has simple and convenient preparation, an even grain size, a large specific surface area and pore volume, and high absorption selectivity. The polymer can be used for carrying out the experiment directly in the water environment without excessive other organic agents. The polymer is environmentally friendly and has a high absorption speed.

The invention discloses a carbon nano tube surface lysozyme molecularly-imprinted polymer. The carbon nano tube surface lysozyme molecularly-imprinted polymer adopts lysozyme as a template molecule, adopts 1-allyl-3-methylimidazolium hexafluorophate ionic liquid and N-tert-butyl acrylamide as a mixing functional monomer, adopts an acrylamide modified carbon nanotube as a matrix, adopts a Tris-HCl buffer solution with the pH being 9 as a reaction system, is obtained under the effect of a cross-linking agent, an initiating agent and a catalyst and has the selective recognition capacity for lysozyme. The number of acting sites is multiple when the template molecule and the functional monomer are combined, the acting force is higher, more effective recognition sites can be formed in the preparation process of the molecularly-imprinted polymer, and therefore the printing effect and the preferential adsorption capacity for lysozyme are better.

The invention relates to a frontal polymerization preparation method for a thymopentin molecular imprinted hydrogel which is prepared from the raw materials in percentage by mass: 4.33%-11.94% of thymopentin, 10.00%-36.44% of N-isopropylacrylamide, 5.88%-21.86% of acrylic acid, 0.36%-0.74% of N, N'-methylenebisacrylamide, 9.92%-33.58% of choline chloride and 30.12%-53.68% of ethylene glycol. By using the frontal polymerization preparation method, a molecular imprinted hydrogel sustained-release material is prepared by taking bioactive molecule thymopentin as a template and a green solvent DESsystem as a pore-foaming agent, and the molecular imprinted hydrogel sustained-release material serving as a drug carrier is stable in physical and chemical properties and obvious in imprinting effect; the molecular imprinted hydrogel sustained-release material is large in drug loading capacity and has an obvious sustained-release effect for the template molecule thymopentin; and compared with a thermal polymerization imprinted hydrogel, the molecular imprinted hydrogel sustained-release material is capable of prolonging the in-vivo metabolism time of a drug and increasing the biological availability of the drug.

The invention relates to an 18beta-glycyrrhetinic acid molecularly imprinted polymer with metal ions as a bridging agent and a monolithic column. The polymer is used as an adsorbing agent for solid phase extraction and separation of glycyrrhetinic acid epimers. The polymer is prepared from 1.55-4.12% of 18beta-glycyrrhetinic acid, 0.82-2.18% of cobaltous acetate, 2.73-2.80% of 4-vinyl pyridine, 0.26-0.32% of azodiisobutyronitrile, 15.63-31.25% of ethylene glycol dimethacrylate, 43.28-43.80% of 1-butyl-3-methylimidazolium tetrafluoroborate, 3.18-3.59% of N,N-dimethylformamide and 18.47-20.01% of dimethyl sulfoxide. Compared with an imprinted polymer with no metal ion, the imprinting factor of the polymer synthesized through the method is improved by about 3 times, and the polymer is dried, ground and screened (74 micrometers) to be used as an adsorbing agent to be placed into an SPE column for carrying out separation and enrichment on 18beta-glycyrrhetinic acid in glycyrrhetinic acid crude extract. The recovery rate of the obtained sample is 91.1%, and the purity of the obtained sample is 93.8%. Accordingly, the polymer can be used for separation, purification and enrichment of the glycyrrhetinic acid crude extract.

The invention discloses a method for preparing a cobalt (II) ion imprinting composite membrane with N-pyrrolidinyl acrylamide and belongs to the scientific and technical field of membrane materials. The method combines metal ion imprinting technology and membrane separating technology, saves complicated operations such as grinding and screening and achieves protection over specific recognition sites, thereby overcoming the shortcomings of complex processes and long elution time in existing cobalt ion imprinting polymer preparation. The method for preparing the cobalt (II) ion imprinting composite membrane with the N-pyrrolidinyl acrylamide is based on the principle that metal ions and compounds of heteroatoms such as N and O can produce relatively good chelation, takes the N-pyrrolidinyl acrylamide as the functional monomer, cobalt (II) as the template ion and a commercial membrane as the carrier to prepare the cobalt (II) ion imprinting composite membrane (Co (II)-MICM). The preparedcobalt (II) ion imprinting composite membrane has the advantages of being stable in performance, high in adsorbing capacity and the like.

The invention discloses a method for preparing an adsorbent for selectively adsorbing crotonaldehyde in cigarette smoke. N-butanal is used as a dummy template, a methacrylic acid, acrylamide, 2-vinylpyridine and 4-vinylpyridine are used as functional monomers, methanol, 2- chloromethane, chloroform and tetrahydrofuran are used as porogen solvents, ethylene glycol dimethyl acrylic ester is used as a cross-linking agent, silica gel is used as a sacrifice monomer, and a polymer which has a selective adsorption function on the crotonaldehyde is synthesized by a sacrificial silica-gel method. The adsorbent has the advantages that the original shape of a silica gel microsphere is maintained, pore volume and specific surface area are larger, reaction sites are numerous, and high selectivity and adsorption capacity on the crotonaldehyde are achieved. The adsorbent is applied to ternary composite filter rods, when the addition amount is 10mg per rod, the reduction rate of the crotonaldehyde is 21.9%, the relative reduction rate of relative tar is 10.1%, and when the addition amount is 20mg per rod, the reduction rate of the crotonaldehyde is 46.0%, and the relative reduction rate of the relative tar is 25.8%.

The invention provides a semi-covalent molecularly imprinted polymer used for selectivity separating phenols, a preparation method of the polymer, and an application of the polymer. According to the invention, template molecules are dissolved in a pore forming solution of a cross-linking agent and an initiator, such that a mixed liquid is prepared. The mixture is subject to a reaction for 18 to 24 hours under a temperature of 55 to 65 DEG C. Obtained white polymer blocks are grinded and screened, such that a polymer with a particle size of 45 to 63 mum is obtained. The polymer is hydrolyzed, such that template molecules are removed. Residual template molecules and interferents are removed through Soxhlet extraction with methanol/acetate and methanol as an extraction solvent, such that the semi-covalent molecularly imprinted polymer is obtained. The invention provides the semi-covalent preparation method of the molecularly imprinted polymer, and the application of the molecularly imprinted polymer in the enriching and/or separating of phenolic compounds. Also, the invention provides a chromatographic column filling material and a solid phase chromatographic column filling material, which are the semi-covalent molecularly imprinted polymers. The molecularly imprinted polymer has a specific selectivity towards phenolic compounds in environmental water samples. Therefore, the polymer can be applied in fast, sensitive, accurate and highly efficient detections of environmental water samples.

The invention belongs to the technical field of functional material preparation, and more specifically relates to a preparation method and applications of click chemistry based lincomycin molecularlyimprinted composite membrane. The preparation method comprises following steps: dopamine and polyethyleneimine are taken as hydrophilic modification materials, Lincomycin is taken as template molecule, 4-vinylpyridine is taken as a functional monomer, Pentaerythritol Tetra(3-mercaptopropionate) is taken as a crosslinking agent, Dipentaerythritol penta-/hexa-acrylate is taken as an auxiliary crosslinking agent, based on click chemistry polymerization method, the lincomycin molecule composite film is prepared. The prepared click chemistry based lincomycin molecularly imprinted composite membraneis capable of solving problems in the prior art that recycling of conventional lincomycin molecule imprinting polymer is difficult, and secondary pollution is easily caused; and in addition, the click chemistry based lincomycin molecularly imprinted composite membrane possesses excellent specific recognition capacity and adsorption separation capacity on Lincomycin.

The invention relates to a method for preparing a monolithic liquid crystal molecular imprinting column by using chiral molecules as a doping agent. A synthesis method is a surface imprinting method. The method comprises the following steps: adding a liquid crystal monomer and a chiral molecule doping agent into a polymerization system, and injecting a pre-polymerization mixed solution in a monolithic polymer framework material column synthesized in advance for polymerization to obtain a low-crosslinking molecular imprinting polymer (MIP) which has a fixed hole size and shape and has functions of determining arrangement of functional groups and identifying template molecules, wherein with the added chiral doping agent, the liquid crystal monomer MPDE (Methyl Phenyl Dicyclohexyl Ethylene) can be converted into a cholesteric phase from a nematic phase, and the imprinting identifying effect is enhanced by adopting an identifying hole generated by a helix and a hole generated by imprinting. The monolithic liquid crystal molecular imprinting column prepared by adopting the method has a good imprinting effect, an imprinting factor reaches up to 22.3, meanwhile, the defect that the applied liquid crystal monomer does not resist high pressure is overcome, and the permeability is high.

The invention relates to a low-template-consumption punicalin imprinting method based on the dual-crowding effect.According to the method, polystyrene and polyethylene glycol serve as two macromolecule crowding agents, an extremely small amount of punicalin serves as template molecules, a functional monomer and a cross-linking agent or initiator are added to a mixed pore-foaming agent formed by methylbenzene and a tetrahydrofuran solution containing the two crowding agents polystyrene and polyethylene glycol, and ultrasonic treatment is conducted to promote dissolution, remove oxygen dissolved in the mixture and induces reaction, so that a selective punicalin imprinted monolithic column is obtained.Template consumption is extremely low, and punicalin imprinting cost is reduced greatly.The punicalin imprinted monolithic column and a blank control column are successfully synthesized in a stainless steel column jacket, and chromatographic performance optimization is conducted.Results show that imprinting factor can be as high as 3.14. The imprinting method is simple, the durability of high-molecular polymers is high, and a low-cost method is provided for separation and purification of punicalin.

The invention relates to preparation of a molecular imprinting release controlled drug carrier through taking metal and organic gel as a pore-foaming agent, in particular to a levofloxacin release controlled molecular imprinting polymer material which takes ferric iron and trimesic acid (Fe<3+>-BTC) metal and organic gel as the pore-foaming agent and a preparation method thereof. The raw materials comprise, by mass, 0.66-1.3% of levofloxacin, 18-27% of ethyldiol methacrylate, 1.7-2.5% of methacrylic acid, 0.14-0.16% of azodiisobutyronitrile, 4.8-5.5% of ferric nitrate, 1.6-1.9% of the trimesic acid and 63-73% of absolute ethyl alcohol, wherein the mass ratio of the ferric nitrate to the trimesic acid is three to one. According to the preparation of the molecular imprinting release controlled drug carrier through taking metal and organic gel as the pore-foaming agent, the slow-release controlling effect of the MIP carrier is good, compared with the release time (5 h) of a non-imprinting polymer-drug, the MIP carrier can release the levofloxacin for more than 16 h, the physical and chemical properties are stable, and therefore a foundation is laid for the molecular imprinting technique to be applied to a drug delivery system.