Clenbuterol hydrochloride molecularly imprinted polymer and preparation method thereof

A clenbuterol hydrochloride and molecularly imprinted technology, which is applied in the field of clenbuterol hydrochloride molecularly imprinted polymer and its preparation, can solve the problems of blurred detection line color, invisible quality control line and high missed detection rate, To achieve the effect of good blotting effect, simple and fast synthesis, and low cost

A clenbuterol hydrochloride and molecularly imprinted technology, which is applied in the field of clenbuterol hydrochloride molecularly imprinted polymer and its preparation, can solve the problems of blurred detection line color, invisible quality control line and high missed detection rate, To achieve the effect of good blotting effect, simple and fast synthesis, and low cost

CN104945552AInactive Publication Date: 2015-09-30GUILIN UNIVERSITY OF TECHNOLOGY +1
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  • Clenbuterol hydrochloride molecularly imprinted polymer and preparation method thereof
  • Clenbuterol hydrochloride molecularly imprinted polymer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of clenbuterol hydrochloride molecularly imprinted polymer, comprising the following steps:

[0026] 1. The preparation concentration is 3×10 -4 The clenbuterol hydrochloride aqueous solution of mol / L;

[0027] 2. Add 5 μL of α-methacrylic acid to 20 mL at a concentration of 3 x 10 -4 mol / L clenbuterol hydrochloride aqueous solution, ultrasonic treatment for 10 minutes, then add 0.0028g N, N-methylenebisacrylamide, continue ultrasonic treatment for 20 minutes, stand overnight, to get the mixed solution (the Clenbuterol hydrochloride Tero, α-methacrylic acid, N, the molar ratio of N-methylenebisacrylamide addition is 9.78:1:3.03);

[0028] 3. Slowly add 100 μL of ammonium persulfate solution with a concentration of 0.5 mol / L to the mixed solution obtained in step 2, and ultrasonically treat it for 15 minutes to initiate a polymerization reaction to obtain a clenbuterol hydrochloride molecularly imprinted polymer.

Embodiment 2

[0030] A preparation method of clenbuterol hydrochloride molecularly imprinted polymer, comprising the following steps:

[0031] 1. The preparation concentration is 3×10 -5 The clenbuterol hydrochloride aqueous solution of mol / L;

[0032] 2. Add 3 μL of α-methacrylic acid to 10 mL to a concentration of 3 x 10 -5 mol / L clenbuterol hydrochloride aqueous solution, sonicated for 5 minutes, then added 0.0014g N,N-methylenebisacrylamide, continued sonicated for 10 minutes, left standing overnight to obtain a mixed solution (the Clenbuterol hydrochloride Tero, α-methacrylic acid, N, the molar ratio of N-methylenebisacrylamide addition is 117:1:30);

[0033] 3. Slowly add 80 μL of ammonium persulfate solution with a concentration of 0.5 mol / L to the mixed solution obtained in step 2, and ultrasonically treat for 10 minutes to initiate a polymerization reaction to obtain a clenbuterol hydrochloride molecularly imprinted polymer.

Embodiment 3

[0035] A preparation method of clenbuterol hydrochloride molecularly imprinted polymer, comprising the following steps:

[0036] 1. The preparation concentration is 3×10 -3 The clenbuterol hydrochloride aqueous solution of mol / L;

[0037] 2. Add 7 μL of α-methacrylic acid to 30 mL at a concentration of 3 x 10 -3 mol / L clenbuterol hydrochloride aqueous solution, sonicated for 15 minutes, then added 0.0042g N, N-methylenebisacrylamide, continued sonicated for 30 minutes, left standing overnight, to obtain a mixed solution (the Clenbuterol hydrochloride Tero, α-methacrylic acid, N, the molar ratio of N-methylenebisacrylamide addition is 274:300:90);

[0038] 3. Slowly add 120 μL of ammonium persulfate solution with a concentration of 0.5 mol / L to the mixed solution obtained in step 2, and ultrasonically treat it for 20 minutes to initiate a polymerization reaction to obtain a clenbuterol hydrochloride molecularly imprinted polymer.

[0039] 2. Analysis of Clenbuterol Hydrochlori...

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Abstract

The invention discloses a method for synthesizing a clenbuterol hydrochloride molecularly imprinted polymer. The method comprises the following step: by using clenbuterol hydrochloride as a template molecule and alpha-methacrylic acid as a functional monomer and performing interaction in the form of hydrogen bonds, adding a crosslinking agent N,N-methylene-bis acrylamide and an initiator ammonium persulfate to perform free radical copolymerization, thereby obtaining the clenbuterol hydrochloride molecularly imprinted polymer, wherein the mole ratio of the template molecule, functional monomer and crosslinking agent is (117-274):(1-300):(30-90). The clenbuterol hydrochloride molecularly imprinted polymer has the advantages of simple and quick synthesis process, low cost, high specificity and favorable stability, can be combined with a test paper method to prepare a test paper strip, and is used for trace on-line detection of clenbuterol hydrochloride.

Description

technical field [0001] The invention relates to the technical field of molecular imprinting, in particular to a clenbuterol hydrochloride molecular imprinting polymer and a preparation method thereof. Background technique [0002] Clenbuterol is a general term for a class of drugs with adrenergic receptor stimulant effects, also known as β2-agonists, which can promote muscle growth and increase lean meat percentage. It has been widely concerned as a growth additive, but β2-receptors Agonists have a certain residual time in animals, and after entering the human body through the food chain, some susceptible people will have obvious symptoms of poisoning, endangering human health. Clenbuterol (CLB) is a kind of clenbuterol, it can significantly promote animal growth and increase lean meat percentage, so it is used by some criminals in animal production to increase lean meat percentage. Studies have shown that clenbuterol hydrochloride remaining in animals will poison the kidne...

Claims

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Application Information

Patent Timeline
30 Sep 2015
Publication
CN104945552A
IPC
C08F220/06; C08F222/38; C08J9/26; B01J20/26; B01J20/30; G01N30/00
Inventors
李建平; 张兰