34results about How to "Rapid Quantitative Assay" patented technology

The invention relates to an active sludge online computer image analysis and early warning system and an active sludge online computer image analysis and early warning method thereof. The system consists of a sample diluter, a peristaltic pump, a computer, a high-speed camera, a microscope and a sample chamber. The method comprises the steps of acquiring a morphological image of a sample firstly and then processing the acquired image; analyzing floc size distribution and filamentous bacterium length distribution; associating the floc particle size distribution and filamentous bacterium length distribution of active sludge with settling properties of the active sludge so as to perform early warning on the settling effect of the active sludge.

An FCM based method for quickly and quantitatively detecting total viruses in a freshwater environment belongs to the field of biotechnology and water environment monitoring, and comprises six main steps including film filtration, settlement, storage, coloration, dilution and detection. The specific operation processes are as follows: passing a sampled water sample through a 0.22 micrometer filter film; adding glutaraldehyde, settling glutaraldehyde for 15 minutes at the temperature of 4 DEG C, and then refrigerating glutaraldehyde by liquid nitrogen; storing glutaraldehyde in a refrigerator at the temperature of minus 80 DEG C; adding Na2EDTA and fluorescent dye SYBRGreen I before detection, and performing coloration for 10 minutes at the temperature of 80 DEG C; diluting the sample by Milli-Q water after the sample cools down to the room temperature; and using the flow cytometer to detect. The method disclosed by the invention overcomes the problem that the present detection method for freshwater total viruses is time consuming and labor consuming, realizes quick and quantitative detection of the freshwater viruses, and has a wide application prospect in fields such as freshwater virus detection and aquatic bioecology study.

The invention discloses a quantum dot-based method for detecting ciprofloxacin by immunofluorescence and a special kit. The immunofluorescence detection kit for detecting the ciprofloxacin comprises a ciprofloxacin peridium and a ciprofloxacin antibody marked by a quantum dot. By the method, the quantitative detection of ciprofloxacin residual medicines can be realized, the detection limit is low, the detection sensitivity is high, the specificity is high, and the method is suitable for detecting various samples. Therefore, the method has a bright application prospect in the field of the detection of the ciprofloxacin.

The invention discloses a method for determining various organic phosphates in surface water by ultra performance liquid chromatography-mass spectrometry. The method includes the main steps of (1) preparing a gradient standard mixed working solution; (2) processing a sample before liquid-liquid extraction; (3) determining a standard solution and a sample solution in the ultra performance liquid chromatography-mass spectrometry; (4) obtaining a standard curve of an organophosphate monomer by standard solution test data and obtaining the content of organophosphate ester in the sample through a standard curve of sample liquid test data. The sample preprocessing is simple, the small column clogging problem is effectively solved, and the small column polarity selection range is wide. The 11 kinds of organic phosphates are qualitatively and quantitatively determined by the ultra performance liquid chromatography-mass spectrometry to achieve good separation of all the organic phosphates within 17 minutes. The target blank adding standard recovery rate is 70%-120% (except for triethyl phosphate 63%), and the test limit is 0.08-1.8ng / L.

The invention provides a method for rapidly measuring the biological activity of an IL-33 / ST2 pathway inhibitor. The method comprises the following steps: constructing a cell strain of a stably expressed NF-kappa B or IL-8 reporter gene, stimulating the reporter gene expression with IL-33, blocking an IL-33 signal pathway by using an IL-33 / ST2 antibody drug, and determining the biological activityof the antibody by fitting a four-parameter curve based on a measured reporter gene signal value. According to the method provided by the invention, a quicker and more accurate quantitative detectionmethod is established for the biological activity of the IL-33 / ST2 antibody drug, the experimental period is short, the operation is simple and convenient, and the cell pollution and errors caused bylong-time incubation and multi-step operation are avoided.

The invention discloses a method for synthesizing a clenbuterol hydrochloride molecularly imprinted polymer. The method comprises the following step: by using clenbuterol hydrochloride as a template molecule and alpha-methacrylic acid as a functional monomer and performing interaction in the form of hydrogen bonds, adding a crosslinking agent N,N-methylene-bis acrylamide and an initiator ammonium persulfate to perform free radical copolymerization, thereby obtaining the clenbuterol hydrochloride molecularly imprinted polymer, wherein the mole ratio of the template molecule, functional monomer and crosslinking agent is (117-274):(1-300):(30-90). The clenbuterol hydrochloride molecularly imprinted polymer has the advantages of simple and quick synthesis process, low cost, high specificity and favorable stability, can be combined with a test paper method to prepare a test paper strip, and is used for trace on-line detection of clenbuterol hydrochloride.

The invention discloses an immunofluorescence detection method for the detection of enrofloxacin based on quantum dots and a special kit thereof. The immunofluorescence detection kit for the detection of enrofloxacin, which is provided in the invention, comprises an enrofloxacin coating antigen and a quantum dot labeled enrofloxacin antibody. The method of the invention, which can realize the quantitative detection of enrofloxacin residues and has the advantages of low detection limit, high detection sensitivity and good specificity, is also suitable for the detection of various samples. So the method and the kit thereof have wide application prospects in the enrofloxacin detection fields.

The invention relates to a method for measuring the green leaf rate of herbaceous plant, and aims at providing a method for quantitatively measuring the green leaf rate of herbaceous plant quickly based the HSV color space. The method comprises the steps: obtaining a quadrat picture through a digital camera; carrying out the preprocessing of the quadrat picture, cutting a representative part in the picture, and storing the representative part; storing the picture in Matlab software, and converting the image into an HSV mode; defining a color section where green leaves are located in the picture as a target color section, and setting the component range of the target color section; carrying out the traversal of all pixels in the picture, and judging whether the pixels are located in the target color section or not; carrying out the statistics and calculation so as to obtain the green leaf rate of a region corresponding to the quadrat picture. The method can save a great amount of manpower and a great number of material resources, is simple and convenient, is reliable in result, and can quickly and accurately obtains the information of the green leaf rate of the herbaceous plant. The method irons out the defects that a conventional method for measuring the green leaf rate of herbaceous plant is poor in subjectivity, roughness and repeatability and the cost is high when professional software is used for digital image processing and measurement.

The invention provides a detection kit for determining lipoprotein related phospholipase A2 by virtue of a quantitative immunosuppression method and application. The detection kit comprises an Lp-PLA2 R1 reagent and an Lp-PLA2 R2 reagent, wherein the Lp-PLA2 R1 reagent contains a buffer matter pair, a preservative and water; and the Lp-PLA2 R2 reagent contains buffer matters, an anti-human Lp-PLA2 enzyme activity antibody, 1-tetanoyl-2-(4-nitrophenyl succinyl)lecithin, 1-nonane sulfonate, vitamin E, a preservative and water. The detection kit can be applied to the detection of the content of lipoprotein related phospholipase A2 in a sample. The detection kit has the characteristics of good stability, high testing efficiency and sensitivity, and a test result is accurate.

The invention belongs to the field of analytical chemistry detection, and particularly relates to a liquid chromatography detection method of BC-02. According to the method, methanol-water is taken as a phase A, and acetonitrile-water-trifluoroacetic acid (TFA)-triethylamine (TEA) which is a moving phase is taken as a phase B; due to appropriate proportion adjustment, the requirement of the separation degree of the BC-02 and the adjacent impurity peak can be met, and main degradation products including hydroxymethyl 5-Fu and 5-Fu can be well separated. Under the same chromatographic condition, the method not only is capable of rapidly detecting the content of the BC-02, but also is capable of detecting the isomer of the BC-02 as well as impurities with structures which are the similar to that of the BC-02 at the same time; after the method is used, the proper sample solution concentration is determined, the lower limit of detection is reduced, and quantitative analysis can be well carried out when the concentration of the detected target is lower, so that the detection method has higher practical application values; furthermore, the method is simple and easy to operate, an auxiliary reagent is low in price and easy to obtain, and the detection results are real and reliable; therefore, the method is a rapid and accurate quantitative detection method.

The invention provides a method for testing quality of medicine for treating alzheimer's disease. The medicine is prepared from the following raw material medicines in parts by weight: 25-75 parts of alpinia oxyphylla miq, 50-150 parts of dodder, 50-150 parts of milkwort, 50-150 parts of dried rehmannia root, 15-45 parts of schisandra, 50-150 parts of ligusticum wallichii, and 50-150 parts of cape jasmine fruit. The quality control method of the medicine comprises a film discrimination method and a quantitative detection method. The detection method provided by the invention is not limited by evaporability and thermal stability of a sample, and is wide in application range; the mobile phases are various, and can be optimized to achieve a high separating efficiency; generally, analysis is carried out at the room temperature; high column temperature is not required; a high-performance liquid chromatography is an accurate fast quantitative detection method for gardenoside, and can make a quality standard for gardenoside.

A quantitative determination method for DNA with quantum point as fluorescent tracer belongs to the DNA detecting domain. The invention builds the standard detection curve making use of that the quantum point has excellent fluorescent characteristic, DNA can quench the fluorescent signal of the quantum point and the signal quenched degree and the DNA quantity construct positive correlation, and obtains the DNA concentration of the solution using the fluorescent intensity and the standard curve of the quantum point in the measuring solution; the signal quenched degree and the DNA quantity construct positive correlation and the specific numerical relationship is: DNA concentration equals to 10(1264.4-Y) / 1786ng / mL; the DNA concentration of the solution can be calculated using the fluorescent intensity and the standard curve of the quantum point in the measuring solution. The invention can be used in calculation of fluorescence labeling DNA quantity, and the method is simple, fast and sensitive.

The invention discloses a method for detecting effectiveness of a domestic drinking water disinfection process. The method comprises the step of detecting the microbial content in purified water treated by a water supply plant disinfection technique by using a flow cytometer. The method disclosed by the invention is simple and feasible, is simple to operate, and can quickly perform quantitative determination to obtain accurate evaluation and monitoring results. The method for detecting the effectiveness of the purified water disinfection technique can master the subsequent addition time and addition amount of sodium hypochlorite more accurately, is more scientific and effective than the existing disinfectant addition mode, and can save a certain consumption of the disinfectant, thereby lowering the operating cost of the water supply plant.

The invention relates to a detection method for liquid chromatogram of keto-L-gulonic acid and / or keto-L-gulonic acid methyl ester. Detection results of a standard sample and a sample to be detected can be respectively obtained by means of detection through a liquid chromatogram mode under the same condition, and contents of the keto-L-gulonic acid and / or the keto-L-gulonic acid methyl ester in the sample to be detected is calculated out through an external standard method. Under the condition of the same chromatogram, the detection method can not only respectively detect the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester independently, but also detect mixture of the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester, thereby establishing a quick and accurate quantitative detection method. The detection method is especially suitable for detecting quantity of surplus keto-L-gulonic acid and the keto-L-gulonic acid methyl ester in methyl esterification reaction coarse products of the keto-L-gulonic acid, and can quantitatively and accurately inspect the reaction process and yield of target products. The detection method is simple and easy to operate, and detection results are authentic and reliable.

The invention belongs to the technical field of chromatographic detection, and specifically relates to a method for quickly detecting vancomycin by utilizing LC-MS (Liquid Chromatography-Mass Spectrometry). The method comprises the following steps: smashing a to-be-detected sample, adding an extracting reagent, and uniformly mixing; then carrying out ultrasonic extraction, and centrifuging, thus obtaining supernatant; extracting the supernatant by adopting water saturated normal hexane, thus obtaining a to-be-purified solution; activating through an MCX solid-phase extraction column, taking and sampling the to-be-purified solution, carrying out drip washing and impurity removing by using methanol, then eluting by using amonium methanol, and collecting an eluent; concentrating, forming a constant volume by using a constant volume solution, and filtering, thus obtaining a purified solution; detecting the purified solution by adopting an HPLC-MS (High Performance Liquid Chromatograph-MassSpectrometer). The method disclosed by the invention has the advantages that the problems of large matrix effects, low sensitivity and the like existing in a process of detecting animal derived foodand particularly the animal derived food with a high fat content in the prior art are well solved, ideal recovery and precision degree are obtained, the minimum detection limit of the method is 0.3 mug / kg, and repeatability and reproducibility of an analysis result are effectively ensured.

The invention relates to the field of chemical detection, and particularly discloses test paper for rapidly detecting the content of urea and application of the test paper. A test paper soaking solution contains potassium thiocyanate, p-dimethylaminobenzaldehyde, tartrate and ascorbic acid. The detection wavelength of the test paper on the photoelectric color photometer is 420-450 nm, and the reaction time is 5-15 min. The method has the advantages of being sensitive in reaction, convenient to operate and the like, and detection accuracy and sensitivity are high.

The invention provides a method for determining the biological activity of a human IL-36 / IL36R / IL1RAcP pathway inhibitor. The method comprises the following steps of: with a cell for stably expressingIL36R, IL1RacP and NF-kappa B reporter genes as an effector cell, mixing the reporter gene with IL-36 and a human IL-36 / IL36R / IL1RAcP pathway inhibitor, incubating, and determining the biological activity of the human IL-36 / IL36R / IL1RAcP pathway inhibitor according to the measured signal value of the reporter gene. The determination method provided by the invention does not need any human primarytissue-derived cells or other components, has the advantages of stable color development result, short experimental period and simple operation, and avoids cell pollution and errors caused by long-time incubation and multi-step operation; in addition, the measured biological activity is related to the clinical curative effect, and the technical requirements related to NMPA are met.

The invention discloses a system for measuring biomass thermal conversion gas, and belongs to the technical field of biomass thermal conversion. Comprising a gas supply control unit, a thermal conversion reaction unit, a condensation purification unit and a gas product analysis unit, the gas supply control unit comprises a gas supply part and a flow control part, the thermal conversion reaction unit comprises a thermal reaction device, a transmission device is arranged on the thermal reaction device, and the thermal reaction device is connected with downstream equipment through the transmission device. The condensation purification unit comprises a gas collection bottle group; the invention also provides a detection method for the biomass thermal conversion gas. The thermal conversion device disclosed by the invention is simple to operate and high in working efficiency, can meet the requirements of biomass pyrolysis and gasification experiments, realizes rapid quantitative determination of index gas in pyrolysis and gasification processes, and can be used for basic research of the biomass pyrolysis and gasification processes.

The invention relates to a detection method for liquid chromatogram of keto-L-gulonic acid and / or keto-L-gulonic acid methyl ester. Detection results of a standard sample and a sample to be detected can be respectively obtained by means of detection through a liquid chromatogram mode under the same condition, and contents of the keto-L-gulonic acid and / or the keto-L-gulonic acid methyl ester in the sample to be detected is calculated out through an external standard method. Under the condition of the same chromatogram, the detection method can not only respectively detect the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester independently, but also detect mixture of the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester, thereby establishing a quick and accurate quantitative detection method. The detection method is especially suitable for detecting quantity of surplus keto-L-gulonic acid and the keto-L-gulonic acid methyl ester in methyl esterification reaction coarse products of the keto-L-gulonic acid, and can quantitatively and accurately inspect the reaction process and yield of target products. The detection method is simple and easy to operate, and detection results are authentic and reliable.

An FCM based method for quickly and quantitatively detecting total viruses in a freshwater environment belongs to the field of biotechnology and water environment monitoring, and comprises six main steps including film filtration, settlement, storage, coloration, dilution and detection. The specific operation processes are as follows: passing a sampled water sample through a 0.22 micrometer filter film; adding glutaraldehyde, settling glutaraldehyde for 15 minutes at the temperature of 4 DEG C, and then refrigerating glutaraldehyde by liquid nitrogen; storing glutaraldehyde in a refrigerator at the temperature of minus 80 DEG C; adding Na2EDTA and fluorescent dye SYBRGreen I before detection, and performing coloration for 10 minutes at the temperature of 80 DEG C; diluting the sample by Milli-Q water after the sample cools down to the room temperature; and using the flow cytometer to detect. The method disclosed by the invention overcomes the problem that the present detection method for freshwater total viruses is time consuming and labor consuming, realizes quick and quantitative detection of the freshwater viruses, and has a wide application prospect in fields such as freshwater virus detection and aquatic bioecology study.

The present invention provides a method for determining the biological activity of human IL-36 / IL36R / IL1RAcP pathway inhibitors, the method comprising: using cells stably expressing IL36R, IL1RacP and NF-κB reporter genes as effector cells, Incubate after mixing with IL-36 and human IL-36 / IL36R / IL1RAcP pathway inhibitor, and determine the biological activity of the human IL-36 / IL36R / IL1RAcP pathway inhibitor according to the measured signal value of the reporter gene . The assay method provided by the present invention does not require any human primary tissue-derived cells or other components, the color development result is stable, the experimental period is short, the operation is simple, and the cell contamination and errors caused by long-term incubation and multi-step operation are avoided; Moreover, the measured biological activity is related to the clinical curative effect and meets the relevant technical requirements of NMPA.

The invention discloses a latex immune enhancement turbidimetric kit for detecting peptide C quantitatively. The latex immune enhancement turbidimetric kit for detecting peptide C quantitatively consists of a peptide C R1 reagent, a peptide C R2 reagent and a peptide C calibration product, wherein the peptide C R1 reagent comprises a buffer liquid, a protective agent I, a reaction enhancer and preservatives; the peptide C R2 reagent comprises a buffer liquid, a protective agent I, preservatives and sensitization polystyrene latex particles coated with anti-human peptide C antibodies; the peptide C calibration product comprises a buffer liquid, a protective agent II, preservatives and peptide C recombinant protein. The kit prepared by the invention is suitable for semi-automatic and full-automatic biochemical analyzers and scattering turbidimetric analyzers, has the advantages of simple and rapid operation, high testing accuracy and high automation degree, is suitable for being used clinically and widely, and is especially suitable for rapid and quantitative determination of emergency treatment.

The invention discloses a rapid detection method of an ionic liquid content, and belongs to the field of analysis and detection. A detection reagent is prepared from sodium tungstate, 85% phosphoric acid and concentrated hydrochloric acid, a working curve takes 1-butyl- 3-methylimidazole tetrafluoroborate as a standard, after 4mL of ionic liquid with different concentrations reacts with 1mL of thedetection reagent, a light absorption value is detected at the wavelength of 600nm, and the working curve is made. After a pretreatment supernatant of a water body or soil sample reacts with the detection reagent, the absorbance of the system is measured, and the content of the ionic liquid in the sample is obtained according to the working curve and the dilution ratio. The detection reagent usedin the method is convenient to prepare, low in price and easy to obtain, instruments are common, the detection reaction time is short, the result is obvious, and the method can provide rapid, simple,convenient and economical quantitative determination for quality inspection departments and production enterprises.

The invention relates to an active sludge online computer image analysis and early warning system and an active sludge online computer image analysis and early warning method thereof. The system consists of a sample diluter, a peristaltic pump, a computer, a high-speed camera, a microscope and a sample chamber. The method comprises the steps of acquiring a morphological image of a sample firstly and then processing the acquired image; analyzing floc size distribution and filamentous bacterium length distribution; associating the floc particle size distribution and filamentous bacterium length distribution of active sludge with settling properties of the active sludge so as to perform early warning on the settling effect of the active sludge.

The invention provides a method for rapidly measuring the biological activity of an IL-33 / ST2 pathway inhibitor. The method comprises the following steps: constructing a cell strain of a stably expressed NF-kappa B or IL-8 reporter gene, stimulating the reporter gene expression with IL-33, blocking an IL-33 signal pathway by using an IL-33 / ST2 antibody drug, and determining the biological activityof the antibody by fitting a four-parameter curve based on a measured reporter gene signal value. According to the method provided by the invention, a quicker and more accurate quantitative detectionmethod is established for the biological activity of the IL-33 / ST2 antibody drug, the experimental period is short, the operation is simple and convenient, and the cell pollution and errors caused bylong-time incubation and multi-step operation are avoided.

The invention discloses an immunofluorescence detection method for the detection of enrofloxacin based on quantum dots and a special kit thereof. The immunofluorescence detection kit for the detection of enrofloxacin, which is provided in the invention, comprises an enrofloxacin coating antigen and a quantum dot labeled enrofloxacin antibody. The method of the invention, which can realize the quantitative detection of enrofloxacin residues and has the advantages of low detection limit, high detection sensitivity and good specificity, is also suitable for the detection of various samples. So the method and the kit thereof have wide application prospects in the enrofloxacin detection fields.

The invention relates to a quantitative measurement method of Brazilin. The method comprises the following steps: respectively carrying out sample application of a hematoxylin sample solution and a standard solution on a silica gel plate by using a sample application tube and carrying out developing based on an ascending method by using a mixed solution of chloroform, acetone and formic acid (in avolume ratio of 8 to 4 to 1) as a developing agent; and carrying out ammonia fumigation alkalization, spraying an alchlor solution, carrying out drying, and carrying out thin-layer chromatography scanning measurement by using maximum absorption of the complex at 508nm as a measurement wavelength. According to the quantitative determination method, the Brazilin being an unstable substance can be converted into a stable substance; contents of brazilin in different samples can be successfully separated, identified and measured on a thin-layer plate once; the sensitivity is high; the method is suitable for analyzing a small number of samples; the requirement for pretreatment of the samples is low; the result is accurate and is obtained rapidly.

The invention discloses a method for rapidly measuring the biological activity of an IL-2 protein drug and an anti-CD25 antibody drug. The method constructs C8166 effector cells transfected with a lentivirus (pLV-STAT5-Luc-PGK-Zencin), and passes through After screening, a monoclonal cell line stably expressing the STAT5 reporter gene was obtained, the expression of the reporter gene was stimulated and activated with IL-2 protein drug, and the signal pathway stimulated and activated by IL-2 was blocked with anti-CD25 antibody drug, and the signal value was fitted according to the measured signal value The biological activity of IL-2 protein drug and anti-CD25 antibody drug was determined by four-parameter curve. The detection method provided by the invention can evaluate the relevant indications of drugs on the downstream of cell signals, does not require any cells or other components derived from human primary tissues, has stable color development, short experimental period, simple operation, and low cost.