34results about How to "Rapid Quantitative Assay" patented technology

The invention belongs to the technical field of chromatographic detection, and specifically relates to a method for quickly detecting vancomycin by utilizing LC-MS (Liquid Chromatography-Mass Spectrometry). The method comprises the following steps: smashing a to-be-detected sample, adding an extracting reagent, and uniformly mixing; then carrying out ultrasonic extraction, and centrifuging, thus obtaining supernatant; extracting the supernatant by adopting water saturated normal hexane, thus obtaining a to-be-purified solution; activating through an MCX solid-phase extraction column, taking and sampling the to-be-purified solution, carrying out drip washing and impurity removing by using methanol, then eluting by using amonium methanol, and collecting an eluent; concentrating, forming a constant volume by using a constant volume solution, and filtering, thus obtaining a purified solution; detecting the purified solution by adopting an HPLC-MS (High Performance Liquid Chromatograph-MassSpectrometer). The method disclosed by the invention has the advantages that the problems of large matrix effects, low sensitivity and the like existing in a process of detecting animal derived foodand particularly the animal derived food with a high fat content in the prior art are well solved, ideal recovery and precision degree are obtained, the minimum detection limit of the method is 0.3 mug / kg, and repeatability and reproducibility of an analysis result are effectively ensured.

The invention relates to a detection method for liquid chromatogram of keto-L-gulonic acid and / or keto-L-gulonic acid methyl ester. Detection results of a standard sample and a sample to be detected can be respectively obtained by means of detection through a liquid chromatogram mode under the same condition, and contents of the keto-L-gulonic acid and / or the keto-L-gulonic acid methyl ester in the sample to be detected is calculated out through an external standard method. Under the condition of the same chromatogram, the detection method can not only respectively detect the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester independently, but also detect mixture of the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester, thereby establishing a quick and accurate quantitative detection method. The detection method is especially suitable for detecting quantity of surplus keto-L-gulonic acid and the keto-L-gulonic acid methyl ester in methyl esterification reaction coarse products of the keto-L-gulonic acid, and can quantitatively and accurately inspect the reaction process and yield of target products. The detection method is simple and easy to operate, and detection results are authentic and reliable.

An FCM based method for quickly and quantitatively detecting total viruses in a freshwater environment belongs to the field of biotechnology and water environment monitoring, and comprises six main steps including film filtration, settlement, storage, coloration, dilution and detection. The specific operation processes are as follows: passing a sampled water sample through a 0.22 micrometer filter film; adding glutaraldehyde, settling glutaraldehyde for 15 minutes at the temperature of 4 DEG C, and then refrigerating glutaraldehyde by liquid nitrogen; storing glutaraldehyde in a refrigerator at the temperature of minus 80 DEG C; adding Na2EDTA and fluorescent dye SYBRGreen I before detection, and performing coloration for 10 minutes at the temperature of 80 DEG C; diluting the sample by Milli-Q water after the sample cools down to the room temperature; and using the flow cytometer to detect. The method disclosed by the invention overcomes the problem that the present detection method for freshwater total viruses is time consuming and labor consuming, realizes quick and quantitative detection of the freshwater viruses, and has a wide application prospect in fields such as freshwater virus detection and aquatic bioecology study.

The invention discloses a latex immune enhancement turbidimetric kit for detecting peptide C quantitatively. The latex immune enhancement turbidimetric kit for detecting peptide C quantitatively consists of a peptide C R1 reagent, a peptide C R2 reagent and a peptide C calibration product, wherein the peptide C R1 reagent comprises a buffer liquid, a protective agent I, a reaction enhancer and preservatives; the peptide C R2 reagent comprises a buffer liquid, a protective agent I, preservatives and sensitization polystyrene latex particles coated with anti-human peptide C antibodies; the peptide C calibration product comprises a buffer liquid, a protective agent II, preservatives and peptide C recombinant protein. The kit prepared by the invention is suitable for semi-automatic and full-automatic biochemical analyzers and scattering turbidimetric analyzers, has the advantages of simple and rapid operation, high testing accuracy and high automation degree, is suitable for being used clinically and widely, and is especially suitable for rapid and quantitative determination of emergency treatment.

A fluorescent speed sensing method for quantitatively detecting glycosylated protein is provided. FSS-CSE testing is performed on glycosylated protein standard products, corresponding different migration rates of fluorescein released after glycosylated protein in different concentrations is combined with fluorescent sensing boric acid probe F1@Rp3 in an electric field are obtained, and a linear relation between the glycosylated protein in known concentration and the migration rates of fluorescein is established; then the glycosylated protein in unknown concentration is tested through the same FSS-CSE, the corresponding migration rates of the fluorescein in the electric field are acquired, and the corresponding migration rates of the fluorescein are substituted in the linear relation so that the concentration of the glycosylated protein can be acquired. The glycated hemoglobin (HbA1c) in blood can be fast and quantitatively measured. Besides, the detection speed is high, and only half an hour is needed for measuring the concentration of the glycosylated protein.

The invention discloses a quantum dot-based method for detecting ciprofloxacin by immunofluorescence and a special kit. The immunofluorescence detection kit for detecting the ciprofloxacin comprises a ciprofloxacin peridium and a ciprofloxacin antibody marked by a quantum dot. By the method, the quantitative detection of ciprofloxacin residual medicines can be realized, the detection limit is low, the detection sensitivity is high, the specificity is high, and the method is suitable for detecting various samples. Therefore, the method has a bright application prospect in the field of the detection of the ciprofloxacin.