Detection method for liquid chromatogram of keto-L-gulonic acid and/or keto-L-gulonic acid methyl ester

Abstract

The invention relates to a detection method for liquid chromatogram of keto-L-gulonic acid and / or keto-L-gulonic acid methyl ester. Detection results of a standard sample and a sample to be detected can be respectively obtained by means of detection through a liquid chromatogram mode under the same condition, and contents of the keto-L-gulonic acid and / or the keto-L-gulonic acid methyl ester in the sample to be detected is calculated out through an external standard method. Under the condition of the same chromatogram, the detection method can not only respectively detect the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester independently, but also detect mixture of the keto-L-gulonic acid and the keto-L-gulonic acid methyl ester, thereby establishing a quick and accurate quantitative detection method. The detection method is especially suitable for detecting quantity of surplus keto-L-gulonic acid and the keto-L-gulonic acid methyl ester in methyl esterification reaction coarse products of the keto-L-gulonic acid, and can quantitatively and accurately inspect the reaction process and yield of target products. The detection method is simple and easy to operate, and detection results are authentic and reliable.

Description

technical field

[0001] The invention relates to the field of analytical chemistry detection, in particular to a high-performance liquid chromatography detection method for gulonic acid and / or guronate methyl ester. Background technique

[0002] Vitamin C is a water-soluble vitamin essential to the human body. The main raw material for the production of vitamin C, sorbitol, is fermented, extracted, and concentrated to obtain solid gulonic acid, which is transformed by alkali to obtain crude vitamin C, and then refined to obtain finished vitamin C. 2-Keto-L-methyl guronate (hereinafter referred to as "methyl guronate") is a precursor for the synthesis of vitamin C, and methyl guronate is the product of methyl esterification reaction in the first step of vitamin C base conversion process , this step reaction is an esterification reaction under the action of acid catalysis, which is a reversible reaction. In order to investigate the yield and quality of intermediates, the hydr...

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