The invention discloses a method for observing obstruction of microbial
groundwater seepage and blockage as well as a device thereof. The method comprises the followings steps: (1) adding a
porous medium to an experiment box; (2) regulating
water level of an upstream control water tank to stabilize the
water flow, constantly illuminating with a
light source, and recording light rays from the box; (3) recording the
water level and nutritive substances of the control water tank by a
digital camera and storing the images; (4) quantitatively analyzing microbial aggregation density and
porous medium density, obtaining a sample containing microbe
aqueous solution and the
porous medium from a
sampling valve of the experiment box; and (5) killing microbes by the
ultraviolet ray; wherein, the experiment box is equipped with a valve, a filter screen and a
water storage tank which is connected with a water pump, the water pump is connected with the control water tank, and a water inlet tube is connected with the experiment box. The method helps directly observe and
record the propagation and migration of the microbes in the porous medium, the blockage manner and change of a
water flow passage, obtains the
observation data, facilitates the control of the microbial activity and sampling analysis, and is applicable to
simulation research of the microbial seepage and obstruction problem in a
groundwater source
heat pump system.