13results about How to "Easy for quantitative analysis" patented technology

The utility model discloses a test device for researching feeding habits of eel fry and a using method thereof, the test device comprises a transparent test barrel, a plurality of shading materials with different light transmittances, a food storage device, a water pump, a temperature controller, a control display and a camera, the water pump is detachably fixed on the inner side surface of the test barrel; the temperature controller is arranged in the experiment barrel and is connected to the control display through a circuit, and the set temperature of the temperature controller is adjusted and controlled through the control display; the camera is arranged above the experiment barrel, the angle of the camera can be adjusted, the camera is connected to the control display, and the angle of the camera is controlled through the control display to achieve all-directional shooting and recording and visually present eel larva feeding habit information shot by the camera. The feeding habit of the eel fry can be comprehensively and coherently observed and shot.

The application relates to a surface enhanced Raman scattering (SERS) substrate, a preparation method and application thereof, and belongs to the technical field of Raman spectrum detection. The substrate comprises, from bottom to top, a substrate layer, a distributed Bragg reflector, a chalcogen phase change material functional layer and a nanoscale metal structure layer. The distributed Bragg reflector is composed of a periodic structure stack of medium materials with different refractive indexes. The distributed Bragg reflector is coupled with the nanoscale metal structure layer to excite a Tamm state. The chalcogen phase change material functional layer can realize reversible regulation of amorphous state and crystalline state under external light excitation, changes the Tamm state resonance wavelength to regulate the SERS enhancement effect. The application realizes high-sensitivity detection through double enhancement of the Tamm state and localized surface plasmon resonance, has the characteristics of non-volatility, reconfigurability, high reproducibility and design flexibility, is convenient to operate, stable in performance, and is suitable for fields of chemical biological sensing, laboratory chip systems and optical encryption.

The utility model discloses an ultrasonic scanning device for a plane circumferential weld, which belongs to the field of ultrasonic scanning devices and is characterized in that a positioning device is arranged on a conveying belt and is matched with a scanning mechanism capable of lifting, displacing and positioning and rotating; when the device is used, a workpiece can be positioned on the conveying belt and then moved to the position below the scanning mechanism to be automatically positioned and scanned, the position of the workpiece is positioned through the positioning device on the conveying belt, the laser sensor can detect the center of the positioned workpiece, and then the workpiece is conveyed to a scanning position along with the conveying belt. After being positioned by the laser sensor, the scanning mechanism can adjust and lower the ultrasonic probe to press the workpiece detection part for scanning, and the ultrasonic probe can rotate and move along the positioning part of the laser sensor, so that the automatic scanning of the plane regular circular ring welding seam can be realized, and the uniformity of scanning force and the accuracy of a path are better ensured; and the labor intensity of workers can be reduced.

The utility model relates to the technical field of physical experiment teaching aids, in particular to a simple harmonic motion experiment instrument, which comprises a base, a liquid spraying device, a support arranged on the base and a paper feeding device arranged on the base, the support comprises a cross rod, a first vertical rod and a second vertical rod, the first vertical rod and the second vertical rod are arranged on two opposite sides of the cross rod, and the cross rod is provided with a cycloid and connected with a pendulum ball. A gap exists between the lowest point of the swing ball in the natural state and the base, a spring is fixed to the first vertical rod and is horizontally placed in the natural state, one end of the spring is detachably connected with the swing ball, the liquid spraying device comprises a spraying pipe, and the liquid outlet end of the spraying pipe is fixedly connected with the swing ball; a spring oscillator experiment and a simple pendulum experiment are integrated on one device, so that students can conveniently compare two simple harmonic motion phenomena on the same platform, the essence and generality of the simple harmonic motion can be deeply understood, and the teaching effect is optimized. Meanwhile, through the detachable connection design, quick switching between a spring oscillator experiment and a simple pendulum experiment is facilitated, and different experiment requirements are met.

The invention relates to the technical field of task allocation, and discloses an airport task allocation and optimization system and method based on the Internet of Things. The method comprises the following steps: firstly, acquiring airport ground support task time information to obtain an airport task time set, and deleting abnormal airport task time data by using an outlier factor detection algorithm; constructing an objective function of an airport task allocation model, establishing the airport task allocation model, and constraining the airport task allocation model; taking the airport task allocation model as a fitness function, and allocating and optimizing airport tasks by using a football team training algorithm under the constraint condition of the airport task allocation model; and finally, according to the flight delay condition, the airport tasks are distributed again, and an airport task distribution scheme is dynamically adjusted. According to the method, the airport tasks are allocated and optimized by constructing the airport task allocation model and using a novel meta-heuristic algorithm, and the method is accurate and novel.

The present invention relates to a windshield dirt detection method based on texture features, comprising: step S1, using threshold judgment based on Sobel edge detection algorithm to divide the original image into texture area and non-texture area; step S2, for transparent or semi-transparent dirt, calculating the edge ratio of two images with different edge strengths extracted from the original image to obtain pixel blur, and then determining dirt candidates according to the threshold division of different texture areas; for opaque dirt, threshold judgment is performed in weak texture areas according to RGB values ​​and average edge strength of pixel blocks to calibrate dirt candidates; step S3, removing false pixels from dirt candidates by multi-frame superposition to determine the dirt area and dirt degree. Compared with the prior art, the present invention has the advantages of high efficiency and convenience and can quantitatively analyze the dirt situation of the windshield.

The invention discloses a construction method of a double-labeled cell based on firefly luciferase, which comprises the following steps: adding Puro, P2A and T2A sequences between a tdTomato fluorescent protein and a DNA sequence of the firefly luciferase, constructing a tdToma-P2A-Puro-T2A-fLuc sequence, designing a PCR (Polymerase Chain Reaction) primer, cloning the tdToma-P2A-Puro-T2A-fLuc sequence to a lentiviral vector in a homologous recombination manner, and transforming the lentiviral vector to a host cell, thereby obtaining the double-labeled cell based on the firefly luciferase. The method comprises the following steps: selecting bacterial colonies, carrying out amplification culture, carrying out amplification culture after sequencing is correct, extracting to obtain tdToma-P2A-Puro-T2A-fLuc expression plasmids, carrying out lentivirus packaging in HEK-293T cells by using a four-plasmid system, infecting the cells, and screening by using antibiotics to obtain the firefly luciferase-based double-labeled cells. According to the method, the red fluorescent protein tdTomato, the resistance gene puromycin and the firefly luciferase fLuc are respectively coupled through P2A and T2A elements, so that the combination of static and dynamic two-dimensional detection is realized, the change of cell activity can be efficiently and sensitively represented through the fLuc, the change of the cell quantity can be dynamically monitored in real time through the tdTomato by utilizing an IncuCyte instrument, and the qualitative and quantitative comprehensive analysis is facilitated.

The invention discloses an online sheath insulation performance detector, comprising: a programmable controller, a display, an alarm; a detection rod, a step-down resistor, a voltage transformer, a first amplifying unit, a second amplifying unit, and an A / D conversion unit; the detection rod inputs the detected voltage value to the input end of the voltage transformer through the step-down resistor, and transmits it to the first amplifying unit through the output end of the voltage transformer, the amplified voltage value is amplified by the second amplifying unit, and then transmitted to the A / D conversion unit for analog-to-digital conversion, the converted digital signal is transmitted to the programmable controller for analysis and processing, and displayed by the display, and at the same time, it is determined whether the alarm is issued by the alarm according to the analysis data. The invention is easy to carry and use, can clearly display the voltage value of leakage, is convenient for maintenance personnel to make quantitative analysis, and will automatically alarm when the leakage voltage value exceeds the set value, thereby improving the maintenance quality and efficiency and avoiding the occurrence of safety accidents.

The present invention discloses a coconut strong promoter, a plant expression vector and an application thereof, wherein the nucleotide sequence of the promoter is shown in SEQ ID NO. 1. Compared with known promoters, the promoter provided by the present invention can drive the efficient expression of related genes and is a strong promoter. The present invention also relates to a plant expression vector containing the strong promoter and an application thereof in initiating the expression of a target gene.

The utility model relates to the technical field of simulation test device, concretely relates to a simulation test structure of simulation blasting fissure propagation and gas dissipation, wherein, the simulation test structure of simulation blasting fissure propagation and gas dissipation includes simulation seat and sets up the cannon column on simulation seat, at least one negative pressure chamber is provided in the simulation seat, a plurality of negative pressure pipes are connected on the simulation seat, each negative pressure pipe is communicated with the corresponding negative pressure chamber, and the negative pressure pipe is connected with negative pressure equipment, the impact unit is provided in the cannon column inner bottom, the technical problem that the physical mechanism that the present blasting simulation test device cannot truly simulate the dynamic propagation of original fissure and the dissipation of blast gas along the new fissure because of single structure function is solved.

The invention relates to the technical field of microbiological detection, and provides a micro-fluidic chip and an application method thereof. The micro-fluidic chip comprises a first main body layer, a flexible film and a second main body layer which are laminated and bonded, the first main body layer is provided with a first sample cavity and a first liquid inlet structure, and the first liquid inlet structure is communicated with the first sample cavity; the second main body layer is provided with a driving mechanism and a plurality of liquid inlet parts, and the flexible film is provided with a through groove, so that the first liquid inlet structure communicates with the liquid inlet parts; the driving mechanism is used for driving the flexible film to be jacked up, and when the flexible film is in a falling state, a sample enters the first liquid inlet structure; when the flexible film is in a jacking state, the sample enters the liquid inlet part from the first liquid inlet structure. According to the micro-fluidic chip, the first liquid inlet structure can be driven to feed and discharge liquid by utilizing the deformation of the flexible film, so that the plurality of liquid inlet parts are filled with samples, and the discrete differentiation of the samples is realized.

The present application relates to the technical field of perforation fracturing damage experiment, and is a kind of perforation fracturing full-process cement sheath damage visualization experiment device and method, the former includes kettle body, simulated surrounding rock, casing, perforating gun, kettle body upper side and lower side are respectively equipped with kettle cover and kettle cover, kettle body is sequentially and spacedly equipped with casing, simulated surrounding rock from inside to outside, casing and simulated surrounding rock form cement annulus, simulated surrounding rock and kettle body form confining pressure cavity, confining pressure cavity is communicated with confining pressure tank through confining pressure pipeline, and perforating gun located in the lumen of casing is installed on the lower kettle cover.The present application is reasonable and compact in structure, easy to use, which simulates the formation environment outside the cement sheath and the surrounding rock constraint by heating, confining pressure and the like, realizes the differentiation of perforation damage site and fracturing damage site of cement sheath one and two interfaces through liquid circulation control system and different color calcium ion fluorescent probe, and is convenient for damage area differentiation and quantitative analysis.

The invention provides a plug seedling bowl force measuring platform. The plug seedling bowl force measuring platform comprises a seedling taking end effector, a seedling tray positioning plate, a lifting module, a servo motor, an air compressor, a mechanism support, a force measuring system, a controller and a PC end. The upper end of the seedling taking end effector is connected with the lifting module, the lifting module is mounted at the upper part of the mechanism bracket, the servo motor is connected with the servo driver, and a power output shaft of the servo motor is connected with the lifting module; the seedling tray positioning plate is mounted at the lower part of the mechanism bracket; the air compressor is connected with the seedling taking end effector through an air pipe; the force measuring system is respectively connected with the seedling taking end executor and the PC end, and is used for detecting the clamping force and the disc separating force of the seedling taking end executor on the seedling pot. According to the plug seedling pot force measuring platform provided by the invention, the platform is constructed based on the seedling taking end executor, so that the clamping force and the tray releasing force of the seedling taking end executor on the seedling pot during working can be conveniently analyzed, various types of seedling taking end executors and the seedling pots with different sizes and specifications can be tested, and the adaptability is wide.