The invention discloses functionalized homogeneous particle
porous silicon dioxide microspheres and a preparation method and application thereof. The preparation method comprises the following steps of: (1) pre-preparing homogeneous particle porous
polymer microspheres with determined components, particle diameters and apertures, and performing surface functionalized treatment on the porous
polymer microspheres to obtain the functionalized homogeneous particle porous
polymer microspheres; (2) dispersing the functionalized porous polymer microspheres in
aqueous solution and adding
silicon dioxide precursor to prepare
silicon dioxide / polymer intermediate composite microspheres; (3) heating the
silicon dioxide / polymer intermediate composite microspheres to remove the polymer to obtain the homogeneous particle
porous silicon dioxide microspheres; and (4) performing
surface modification on the homogeneous particle
porous silicon dioxide microspheres by using a chemical
reagent to form a functionalized group, wherein the particle
diameter of the functionalized homogeneous particle porous
silicon dioxide microspheres is within the range of between 1.7 and 100 microns; and the mesoporous aperture of the functionalized homogeneous particle porous
silicon dioxide microspheres is within the range of between 20 and 1,000 angstroms. The functionalized homogeneous particle porous
silicon dioxide microspheres can be used as chromatographic filler for efficiently analyzing and separating
organic molecules and biological molecules.