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Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging

A technology of biomedical materials and functional molecules, applied in the field of bioactivation modification, can solve the problems of loss of biological activity of functional molecules, failure to achieve stable immobilization of functional molecules, etc.

Inactive Publication Date: 2012-12-12
高长有
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, simple physical adsorption cannot achieve the purpose of stably immobilizing functional molecules, while chemical immobilization may cause functional molecules to lose their biological activity.

Method used

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  • Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging
  • Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging
  • Method for fixing functional molecules on biomedical material surface with dopamine serving as bridging

Examples

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example 1

[0025] A method for immobilizing functional molecules on the surface of a biomedical material bridged by dopamine, comprising the following steps:

[0026] (1) Soak the nickel-titanium alloy that has removed the oxide layer and surface stains in a buffer solution containing 2mg / ml dopamine, the buffer solution is 10mM Tris-HCl, its pH value is 8.5, soak for 24 hours, and the reaction temperature is 50 o C, then moved to double distilled water and rinsed for 20 minutes, then dried with nitrogen;

[0027] (2) Soak the dried nickel-titanium alloy immobilized with dopamine in a buffer solution with a concentration of 2mg / ml heparin, the buffer solution is PBS buffer solution, its pH value is 7.4, react for 12 hours, and the soaking reaction temperature is 37 o c. Then move to double-distilled water for rinsing for 10 minutes, and finally blow dry with nitrogen to obtain heparin-immobilized nickel-titanium alloy. After assembling the polydopamine and heparin, the DSA10-MK2 surf...

example 2

[0035] A method for immobilizing functional molecules on the surface of a biomedical material bridged by dopamine, comprising the following steps:

[0036] (1) Soak the cobalt-chromium alloy that removes the oxide layer and surface stains in a buffer solution containing 1mg / ml dopamine, the buffer solution is 10mM Tris-HCl, its pH value is 8.5, soak for 24 hours, and the reaction temperature is 37 o C, then moved to double distilled water and rinsed for 30 minutes, then dried with nitrogen;

[0037] (2) Soak the dried dopamine-fixed cobalt-chromium alloy in a buffer solution with a concentration of 2mg / ml heparin, the buffer solution is PBS buffer solution, and its pH value is 7.4, react for 12 hours, and the soaking reaction temperature is 37 oc. Then move to double-distilled water for rinsing for 10 minutes, and finally blow dry with nitrogen to obtain heparin-immobilized cobalt-chromium alloy.

example 3

[0039] A method for immobilizing functional molecules on the surface of a biomedical material bridged by dopamine, comprising the following steps:

[0040] (1) Soak the nickel-titanium alloy to remove the oxide layer and surface stains in the buffer solution containing 3mg / ml dopamine, the buffer solution is 10mM Tris-HCl, its pH value is 8.5, soak for 12 hours, and the reaction temperature is 37 o C, then moved to triple distilled water and rinsed for 60 minutes, then dried with nitrogen;

[0041] (2) Soak the dried dopamine-fixed nickel-titanium alloy in a buffer solution with a concentration of 3mg / ml heparin, the buffer solution is PBS buffer solution with a pH value of 7.4, react for 12 hours, and the soaking reaction temperature is 37 o c. Then move to distilled water for rinsing for 10 minutes, and finally blow dry with nitrogen to obtain heparin-fixed nickel-titanium alloy.

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Abstract

The invention discloses a method for fixing functional molecules on a biomedical material surface with dopamine serving as a bridging. The method uses the dopamine to serve as a bridging medium, firstly uses strong auto polymerization characters of the dopamine under certain pH environment to be assembled on the biomedical material surface to form a poly-dopamine layer, and then a chemical reaction is used for stem grafting of the functional molecules on the poly-dopamine layer to build the biomedical material surface with biological activity. The method does not damage the biological activity of the functional molecules and can change assembling thickness of the poly-dopamine layer by controlling reaction time so as to change grafting percent of the functional molecules on the surface. The method is simple and practical in process, good in repeatability and capable of widely applying to surface functionalization modification of biomedical materials such as intervention materials and tissue engineering materials, and has good clinical application prospects.

Description

technical field [0001] The invention relates to bioactive modification of substrates for tissue engineering, in particular to a method for immobilizing functional molecules on the surface of biomedical materials with dopamine as a bridge. Background technique [0002] Tissue engineering is an interdisciplinary research topic involving medicine, chemistry, biology, materials science and other disciplines and fields. In the study of tissue engineering, the effective immobilization of functional molecules on the surface of biomedical materials and the construction of bioactive scaffold materials are important links, which directly affect the activity and functional expression of target cells and target proteases. Functional molecules refer to polymer materials with certain specific functions. The reason why they have specific functions is due to the incorporation of specific functional groups in their molecular chains, or the complexation of molecules with other materials with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/28A61L27/54
Inventor 高长有马列刘云云
Owner 高长有
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