Quantitative measurement method of Brazilin

Abstract

The invention relates to a quantitative measurement method of Brazilin. The method comprises the following steps: respectively carrying out sample application of a hematoxylin sample solution and a standard solution on a silica gel plate by using a sample application tube and carrying out developing based on an ascending method by using a mixed solution of chloroform, acetone and formic acid (in avolume ratio of 8 to 4 to 1) as a developing agent; and carrying out ammonia fumigation alkalization, spraying an alchlor solution, carrying out drying, and carrying out thin-layer chromatography scanning measurement by using maximum absorption of the complex at 508nm as a measurement wavelength. According to the quantitative determination method, the Brazilin being an unstable substance can be converted into a stable substance; contents of brazilin in different samples can be successfully separated, identified and measured on a thin-layer plate once; the sensitivity is high; the method is suitable for analyzing a small number of samples; the requirement for pretreatment of the samples is low; the result is accurate and is obtained rapidly.

Description

technical field [0001] The invention relates to a method for determining effective components of Chinese medicinal materials, in particular to a method for quantitatively determining brazilian hematoxylin. Background technique [0002] Sappan (Caesalpinia sappan L.) is a plant of the genus Sappan, and its dried heartwood is a commonly used traditional Chinese medicine. According to the Pharmacopoeia of the People's Republic of China, its properties are: sweet, salty, flat, heart-guiding, liver, and spleen meridians, with blood-dispelling fatigue, swelling and pain relief, and anti-inflammatory effects. swelling and pain. Modern pharmacological screening has also found that hematoxylin ethanol or water extracts have good swelling and pain relief, anti-inflammatory, anti-tumor, and anti-cancer activities. brazilian hematoxylin [(6aS-cis)-7,11 b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10,(6H)-tetrol] is one of the main active ingredients extracted from hematoxylin heartwood,...

AI Technical Summary

Problems solved by technology

These methods generally have problems such as time-consuming operation, expensive equipment, and gradual deepening of color during the measurement process.

However, the simple and easy thin-layer chromatography is mainly used for the qualitative analysis of the substance at present, and the method for the quantitative analysis of brazilian hematoxylin by thin-layer chromatography has not been reported so far.

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