Method for determining contents of 21 types of terpenoids in tobacco leaf and application of method

A technology of terpenoids and content, which is applied in the field of determination of 21 kinds of terpenoids in tobacco leaves, can solve the problems of complex pretreatment and narrow coverage of terpenoids, and achieve the effect of good selectivity, high sensitivity and low interference

Active Publication Date: 2019-04-16
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using this method to detect 15 terpenoids in tobacco, the pretreatment is complex and the coverage of terpenoids detected is still narrow

Method used

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  • Method for determining contents of 21 types of terpenoids in tobacco leaf and application of method
  • Method for determining contents of 21 types of terpenoids in tobacco leaf and application of method
  • Method for determining contents of 21 types of terpenoids in tobacco leaf and application of method

Examples

Experimental program
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Embodiment 1

[0031] Such as figure 1 Shown, the method for the content of 21 kinds of terpenoids in the assay tobacco leaf of the present embodiment comprises the following steps:

[0032] 1. Determination of the standard curve:

[0033] Accurately take by weighing 21 kinds of terpenoid standard items that the present invention measures, dissolve with the dichloromethane solution containing tridecanoic acid (the concentration of tridecanoic acid is 2.5mg / L), be mixed with the mass concentration of each terpenoid Both are 1mg / L standard stock solutions, which are stored at -80°C for future use.

[0034] Then accurately draw the above-mentioned standard stock solution, dilute with a dichloromethane solution containing tridecanoic acid (the concentration of tridecanoic acid is 2.5mg / L) to prepare a series of standard working solutions, and then analyze with GC-MS / MS. Among them, the analysis conditions of GC-MS / MS are as follows: the chromatographic column is DB-5MS gas chromatographic colu...

Embodiment 2

[0047] According to the determination method of Example 1, the terpenoid components in the tobacco variety Yun 87 in Yunnan area were detected, and the measured results are shown in Table 3.

[0048] Table 3 Contents of 21 terpenoids in tobacco leaves of Yun 87 in Yunnan area

[0049]

[0050]

[0051] *Cannot be detected in normal tobacco leaves.

[0052] In other embodiments of the present invention, other tobacco leaf varieties in Yunnan area (such as Kunming Hongda, Qujing Yun 105, etc.), tobacco leaf varieties in Henan area (Xuchang Yuyan No. 10, Pingdingshan Zhongyan 100, Nanyang Zhongyan 100 and The content of terpenoids in Zhumadian Zhongyan 100) and Guizhou tobacco varieties (Zunyi Nanjiang No. 3, Bijie Bina No. 1, Qiannan Guiyan 201 and Tongren K326) were detected and analyzed.

[0053] In other embodiments of the present invention, 1.0-2.0 mL of extract can be added for every 20 mg of fresh tobacco leaf freeze-dried powder. The extraction solution was a dich...

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Abstract

The invention relates to a method for determining contents of 21 types of terpenoids in tobacco leaf and application of the method, and belongs to the techcnial field of tobacco component detection. The method comprises the following steps that the tobacco leaf after freeze-drying is mixed with an internal standard and dichloromethane, extraction is carried out then, solid is separated from liquid, and a solvent is removed from the liquid to obtain an extract; a derivatization reagent is added into the extract for reaction, and a solution to be tested is obtained; and GC-MS/MS analysis is carried out on the solution to be tested, the contents of 21 types of terpenoids are determined, and the contents of the 21 types of terpenoids in tobacco leaf powder are calculated. The determining method covers more terpenoids, the GC-MS/MS method is used, the sensitivity, selectivity and efficience are high, interference is low, and the method can be applied to different types of terpenoids including sesquiterpene, diterpene and sterol.

Description

technical field [0001] The invention belongs to the technical field of tobacco component detection, and in particular relates to a method for measuring the contents of 21 kinds of terpenoids in tobacco leaves and an application thereof. Background technique [0002] Terpenoids are one of the important secondary metabolites in plants, which play an important role in the growth and development of plants and in resisting external aggression. In addition to physiological functions such as regulating tobacco growth and enhancing stress resistance, terpenoids in tobacco are also closely related to the aroma quality of tobacco, and are important indicators for measuring tobacco quality. Therefore, accurate detection of the content of terpenoids in tobacco is an important prerequisite for studying terpenoid metabolic pathways, increasing the content of terpenoids, and analyzing the aroma quality of tobacco. [0003] The detection methods for terpenoids at home and abroad mainly inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 郑庆霞周会娜陈千思刘萍萍魏攀卢鹏翟妞金立锋徐国云张慧王晨金静静
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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