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56results about How to "Suitable for analysis" patented technology

Enzyme-linked immunoassay method for determining content of cell DNA (Deoxyribonucleic Acid) injury marker H2AX

The invention provides an enzyme-linked immunoassay method for determining the content of a cell DNA (Deoxyribonucleic Acid) injury marker H2AX. The enzyme-linked immunoassay method is characterized by comprising the steps that sandwich recognition on the specificity of a target protein H2AX is carried out by using a specific H2AX antibody and an enzyme-linked second antibody, a substrate after enzyme reaction is added, then the substrate is subjected to enzyme catalysis to be changed into a fluorescence product, wherein quantitative determination for the H2AX content can be realized according to a fluorescence value of the substrate. According to the method, the content change of the H2AX in the cells, caused by the fact that cigarette smoke is exposed, is quantitatively determined so that the aim of evaluating the virulence of cigarette smoke genes is realized. Compared with a traditional organic dye, the enzyme-linked antibody has the advantages that the catalyzing frequency of an enzyme is high, an amplification reaction effect is amplified and the detection sensitivity is improved; the enzyme has a stable property at a room temperature, is low in price, and is suitable for a lot of analysis. An enzyme-linked immunosorbent assay has the advantages of rapidness, accuracy, high throughput, high sensitivity and the like.
Owner:CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT

Multidimensional chromatography combination separation device

InactiveCN101762666AResolve resolutionSolving limited peak capacityComponent separationSmall sampleIon exchange
The invention relates to a multidimensional chromatography combination separation device, which comprises a pressurized capillary electrochromatography unit. The pressurized capillary electrochromatography unit comprises a mobile phase driving device, an injection valve, a capillary chromatographic column and a buffer solution bottle which are connected mutually and sequentially, a high-voltage power supply connected in parallel with the capillary chromatographic column, and a detector connected on the capillary chromatographic column. The separation device also comprises a capillary ion-exchange chromatography unit. The capillary ion-exchange chromatography unit comprises a mobile phase driving device, an injection valve and a capillary ion-exchange chromatography column which are connected mutually and sequentially. The capillary ion-exchange chromatography column is connected with an injection port of the injection valve of the pressurized capillary electrochromatography unit. The multidimensional chromatography combination separation device has the advantages of high separation efficiency, large peak capacity, small sample dosage, low raw material cost and low pollution, has more excellent peak capacity and separation capacity than the conventional one-dimensional chromatography, pressurized capillary electrochromatography, two-dimensional liquid chromatography and the like, and is more suitable for analysis of the complex sample.
Owner:UNIMICRO SHANGHAI TECH

Gas chromatograph for gas analysis in production field

The invention discloses a gas chromatograph for gas analysis in production field, comprising a sampling unit, a gas path control unit, a circuit control unit, a separation unit, a detection unit and a data processing unit. The gas chromatograph for gas analysis in production field is characterized in that the sampling unit is a sampling and automatic sample injection unit, and is composed of a fixed volume sampling tube, a flow meter and a ten-way valve with blowing function; the gas path control unit is composed of a solid filter, a stainless steel diaphragm pressure stabilization valve and a helium purifier, and provides high-purity carrier gas for the separation unit; the circuit control unit is composed of a temperature control circuit, a signal amplification circuit and a detector pulse high-voltage circuit; the separation unit is provided with three diaphragm valves with blowing function, wherein one is the ten-way valve with blowing function in the sampling unit, the other two are four-way valves with blowing function, and complete separation of constituents is completed through a three-valve and four-column switching flow; the detection unit is a pulse helium ionization detector; and the data processing unit is composed of an embedded industrial control computer and a data uploading module. The gas chromatograph for gas analysis in production field has the advantages of high detection sensitivity, good stability and strong operability.
Owner:SHANGHAI HUAAI CHROMATOGRAPHIC ANALYSIS

Real-time feature extraction method for analysis of complex ingredient of traditional Chinese medicine

The invention provides a real-time feature extraction method for the analysis of complex ingredients of traditional Chinese medicines. The method adopts a data communication module, a two-dimensional feature chain detection module, a correction module for local noise and local baseline and feature distinguishing module. Mass spectrometric data collected by a sequence-analysis mass synchrometer is used for determining whether the data has continuous features with data collected at the previous time point so as to dynamically detect a two-dimensional feature chain; utilizing the mass-charge ratio and time information contained in the two-dimensional feature chain can quickly remove noises and baselines in time dimension, thereby overcoming the defect that the previous algorithm can not be accurately estimate the baseline by means of time dimension only; as the estimated noise and baseline have local features, a local signal-to-noise ratio is that whether the feature chain contains the feature of a component, thereby simplifying the realization of feature detection. The invention has reasonable design, and the data processing system has the characteristics of real time, fewer custom parameters and high arithmetic speed, which is applicable for liquid chromatograph-mass spectrometers.
Owner:ZHEJIANG UNIV

Detection method for odor substances in exhaust gas of perfume and essence industry

The invention discloses a detection method for odor substances in exhaust gas of the perfume and essence industry. According to the invention, exhaust gas discharged by the perfume and essence industry is directly or indirectly acquired, then solid-phase micro-extraction (SPME) or solid-phase adsorption-thermal desorption technology are employed for extraction of a sample; and extraction temperature and extraction time are adjusted and GC-MC is cooperatively used for analysis of the exhaust gas sample. The detection method is high in efficiency, simple to operate and applicable to analysis of odor substances with any concentration. An airbag method and negative pressure sampling technology are cooperatively used, an appropriate sampling point is selected and a representative gas sample is acquired, so original information of the sample is maintained to a greatest extent. A sampling bag has good airtightness, and a sample in the bag can be maintained for more than one month. SPME pretreatment technology is employed, so the advantages of greenness, rapidness, no pollution and repeated usability can be obtained. The detection method is applicable to both exhaust gas discharged in the perfume and essence industry and to detection of exhaust gas of industries like petroleum, the chemical industry, food, medicine and cosmetics.
Owner:HUIZHOU RES INST OF SUN YAT SEN UNIV

Automatic pickup method of earthquake preliminary wave based on mean shift clustering analysis

The invention discloses an automatic pickup method of an earthquake preliminary wave based on mean shift clustering analysis. The method comprises the steps of: preprocessing earthquake data, converting waveform information into point density information, performing clustering on preprocessed records, giving a channel with a higher signal-to-noise ratio to serve as an initial channel for startingpickup, optimizing clustering centers corresponding to the preliminary wave according to the principle of minimum time difference starting from the initial channel, processing a record obtained from single shot by using a MeanShift clustering algorithm in view of the characteristic that the amplitude of the preliminary wave is the highest, optimizing the clustering result, and obtaining a corresponding first arrival time to serve as an automatic pickup result. According to the automatic pickup method disclosed by the invention, a probability density distribution function of sample data does not need to be known in advance, dependence is completely made on the calculation of sample points, certain convergence is performed in the case that the sampling is sufficient, and density estimationcan be performed on data complying with arbitrary distribution.
Owner:五季数据科技(北京)有限公司 +1

Replicate system of automobile engine steel grade

The invention discloses a replicate system of an automobile engine steel grade, comprising an eddy current probe, an electric displacement platform, a difference circuit, a signal conditioning circuit, a data collector, a trace imaging module and an actuating signal generating and conditioning circuit, wherein the trace imaging module is used for outputting an actuating command signal F45 to the actuating signal generating and conditioning circuit; the actuating signal generating and conditioning circuit is used for transmitting an actuating signal uin to the eddy current probe according to the actuating command signal F45; the eddy current probe is combined with the electric displacement platform to carry out surface-layer defect scan detection on a piece to be tested in order to obtain defect information uout on the tested piece; after the defect information uout passes through the difference circuit, the signal conditioning circuit and the data collector, numerical defect information u3 is transmitted to the trace imaging module; and the trace imaging module carries out surface-layer trace original information restoration of the received numerical defect information u3 to obtain the original actual image information of the tested piece. The replicate system can nondestructively detect magnetic field variation of the automobile engine steel grade due to altering and falsifying traces, thereby replicating the original automobile engine steel grade.
Owner:BEIHANG UNIV

Radioactive aerosol particle recovery device

The invention discloses a radioactive aerosol particle recovery device, comprising an inlet nozzle and an inlet duct; a plurality of impact orifice plates with specific orifice sizes are assembled below the inlet duct in order; a collection carrier for intercepting particle with the determined scope is arranged below each impact orifice plate; a high efficient filter film installed in the filter film liner is arranged below the lowest collection carrier; the particles passing through the collection carrier reach the next impact orifice plate or high efficient filter film via a transitional tube; an exhaust tube nozzle is arranged below the filter film liner; and the exhaust tube nozzle is connected with an exhaust power regulation and control device for wholly operating. In the radioactive aerosol particle recovery device, the particles in the different ranges can be collected on the different carrier; the radioactive aerosol particle recovery device is applicable to radioactive aerosol mass spectrometer measurement with many specific purposes or the related measurement thereof. Furthermore, the recovery device is also applicable to the special site where it is not convenient to sample by using the common aerosol sampling device, so that the recovery device is in accordance with the special sampling and monitoring requirement of certain aerosols.
Owner:CHINA INST FOR RADIATION PROTECTION

Method for determining germanium content of cobalt-nickel slag by atomic fluorescence spectrometry

The invention belongs to the technical field of metallurgical industry analysis and discloses a method for determining germanium content of a cobalt-nickel slag by atomic fluorescence spectrometry. The method comprises the following steps: preparing a germanium standard solution, a zinc standard solution and a thiourea-ascorbic acid mixed solution, and drawing a germanium standard solution curve; weighing a cobalt-nickel slag sample to prepare a sample solution; determining the fluorescence intensity of germanium in the sample by using an atomic fluorescence spectrometer; and comparing with the germanium standard solution curve and analyzing the content of the germanium in the sample solution. According to the method, after the approximate equal amount of zinc is added into the germanium standard solution, the germanium standard curve is drawn to eliminate the inferences to a detection result by the base body zinc. The method disclosed by the invention has the characteristics of low detection limit, wide linear range, low maintenance cost, rapidness in measurement, simplicity in operation, good accuracy, high precision, capability of simultaneously and rapidly determining elements to be detected and the like; furthermore, the method has the characteristics of few needed reagents, low cost and high automation degree, is suitable for analyzing a large batch of samples and has a very good industrial utilization value.
Owner:BAIYIN NONFERROUS GROUP

Dispersive liquid liquid micro-extraction gas chromatography-mass spectrometry for detecting major higher alcohols in fermented wine

The invention discloses a dispersive liquid liquid micro-extraction gas chromatography-mass spectrometry for detecting major higher alcohols in fermented wine and belongs to the technical field of analytical chemistry. The method comprises the following steps: selecting appropriate extraction agents and dispersing agents, mixing a certain amount of extraction agents and dispersing agents with internal standard substances, rapidly injecting the mixture into a wine sample to form emulsion so as to be extracted, centrifugally collecting the extraction phase, performing gas chromatography-mass spectrometry, and quantifying through an external standard method, thereby obtaining analytical results of higher alcohols in the fermented wine. The DLLME (Dispersive Liquid Liquid Microextraction) method used for performing sample pretreatment has the advantages that the dose of an organic solvent is small, the operation is rapid, simple and convenient, the usage amount of the organic solvent is small, the detection result accuracy is high, and the recovery is satisfactory. By considering the advantages of low cost and environment friendliness, the method is easily used in practice, is particularly suitable for analysis of a large batch of samples, and can be applied to detecting the major higher alcohols in multiple kinds of fermented wine.
Owner:JIANGNAN UNIV +2

Nanometer carbon nitride/copper oxide composite material, and nanometer carbon nitride/copper oxide composite solid-phase micro-extractor, preparation method and applications thereof

A purpose of the invention is to provide a nanometer carbon nitride/copper oxide composite material, and a nanometer carbon nitride/copper oxide composite solid-phase micro-extractor, a preparation method and applications thereof. The preparation method of the nanometer carbon nitride/copper oxide composite solid-phase micro-extractor comprises: dispersing nanometer carbon nitride in deionized water, adding a copper nitrate solution while stirring, adding a hexamethylenetetramine solution in a dropwise manner, continuously stirring until the materials are uniformly mixed, stopping the stirring, carrying out a constant temperature reaction for 3-5 h at a temperature of 80-100 DEG C, collecting the precipitate after the reaction is completed, washing, drying to obtain a nanometer carbon nitride/copper oxide composite material, and wrapping the surface of quartz fibers with the nanometer carbon nitride/copper oxide composite material through a silica colloid to prepare the solid-phase micro-extractor. According to the present invention, the nanometer carbon nitride/copper oxide composite material has good adsorption performance on polycyclic aromatic hydrocarbons, and the solid-phasemicro-extractor prepared from the composite material has high extraction efficiency on polycyclic aromatic hydrocarbons, and can meet the detection requirements of polycyclic aromatic hydrocarbons.
Owner:HENAN UNIVERSITY

Method for pretreating sample during detection of impurities in high-purity aluminum metaphosphate by using plasma spectrum method

The invention discloses a method for pretreating a sample during detection of impurities in high-purity aluminum metaphosphate by using a plasma spectrum method, which belongs to the technical field of detection of trace impurity elements, and is used for solving the problems that the impurities are easily introduced, the interference is great and the sample introduction flow fluctuates to influence the accuracy in the conventional method. The method mainly comprises the following steps: (1) weighing a solid-state high-purity aluminum metaphosphate powder sample, adding the sample into a plastic closed container, adding a concentrated hydrochloric acid solution and fully contacting aluminum metaphosphate with the acid solution, closing the plastic closed container, and preserving the digestion temperature to be 180-200 DEG C for 4-5 hours; and (2) if clarification is achieved, cooling to room temperature, transferring the solution into a volumetric flask, diluting the solution to achieve the scales, and shaking uniformly, thereby obtaining an aluminum metaphosphate sample solution; if clarification is not achieved, adding a proper amount of hydrofluoric acid, stirring, standing, diluting the solution to achieve the scales after the solution is clarified, shaking uniformly, thereby obtaining the aluminum metaphosphate sample solution. The method disclosed by the invention has the characteristics of relatively few reagents, low salinity and low spectral background and is mainly used for measuring the trace impurity elements contained in high-purity aluminum metaphosphate.
Owner:HUBEI NEW HUAGUANG NEW INFORMATION MATERIALS CO LTD
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