Multidimensional chromatography combination separation device

Abstract

The invention relates to a multidimensional chromatography combination separation device, which comprises a pressurized capillary electrochromatography unit. The pressurized capillary electrochromatography unit comprises a mobile phase driving device, an injection valve, a capillary chromatographic column and a buffer solution bottle which are connected mutually and sequentially, a high-voltage power supply connected in parallel with the capillary chromatographic column, and a detector connected on the capillary chromatographic column. The separation device also comprises a capillary ion-exchange chromatography unit. The capillary ion-exchange chromatography unit comprises a mobile phase driving device, an injection valve and a capillary ion-exchange chromatography column which are connected mutually and sequentially. The capillary ion-exchange chromatography column is connected with an injection port of the injection valve of the pressurized capillary electrochromatography unit. The multidimensional chromatography combination separation device has the advantages of high separation efficiency, large peak capacity, small sample dosage, low raw material cost and low pollution, has more excellent peak capacity and separation capacity than the conventional one-dimensional chromatography, pressurized capillary electrochromatography, two-dimensional liquid chromatography and the like, and is more suitable for analysis of the complex sample.

Description

technical field [0001] The invention relates to a separation detection device in the field of separation analysis, in particular to a multi-dimensional chromatographic separation device. Background technique [0002] Pressurized capillary electrochromatography (pCEC) is a new type of micro-separation analysis technology developed in recent years. It integrates the advantages of capillary electrophoresis and liquid chromatography. , the retention behavior of the sample in the capillary column is also affected by the electroosmotic flow and its distribution coefficient between the mobile phase and the stationary phase, which greatly improves the separation ability of the sample. Under the action of the dual separation mechanism, pCEC is fine for the separated sample The resolution ability has been greatly improved. [0003] For example, the Chinese invention patent specification with the notification number CN 2373793Y discloses a "multi-purpose pressurized electrochromatogra...

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is a multi-dimensional chromatographic separation device with high separation efficiency, large peak capacity, small sample consumption, low raw material cost and low pollution, so as to overcome the deficiencies in the prior art

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