Detection kit and detection method for hepatitis B virus genotyping

Abstract

The invention discloses a detection kit and a detection method for hepatitis B virus genotyping. The kit comprises a primer pair for polymerase chain reaction (PCR) amplification of a hepatitis B virus gene S area and a PreS2 area, a solid phase detection array, and a nucleic acid amplification reaction system and a color development reagent on a solid phase carrier containing DNA polymerase and reaction substrate, wherein a type detection probe and a contrast probe are fixed on the surface of the solid phase detection array. According to the gene chip-based high-flux, high-specificity, quick and easily-popularized detection method using a PCR technology as the principle, the detection probe is fixed on the detection array and hybridized with a PCR amplified gene fragment to be detected; and after the target fragment is identified by the probe on the solid phase carrier, a nucleotide sequence can be synthesized and prolonged, so that the biotin-labeled amplified fragment is fixed on the detection array, is not eluted and further used for color development and display, and the gene type of the hepatitis B virus to be detected can be detected at one time.

Description

technical field [0001] The invention belongs to the field of medical testing, relates to the type detection of the hepatitis B virus gene, in particular to a detection kit and detection method for the type of hepatitis B virus genotype. Background technique [0002] Viral hepatitis B is a worldwide disease caused by the hepatitis B virus (HBV). The incidence rate is high in developing countries. There are about 93 million hepatitis B virus carriers and patients in my country, and 1 / 3 of them have clinical manifestations of liver damage. At present, there are 30 million hepatitis B patients in our country. After being infected by HBV, it can gradually evolve into more serious liver diseases, such as liver cirrhosis, liver cancer, etc., resulting in 500,000 deaths per year. [0003] Studies in recent years have shown that the different HBV genotypes caused by gene mutations are geographically distributed, and the pathogenicity of different genotypes is different. close rela...

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Problems solved by technology

However, in terms of actual operation, more complex chemical treatments are required for primer modification and immobilization, such as not only the surface treatment of the carrier, but also the modification of the probe or primer, and the matching of the solid phase carrier on the silica gel sheet or capillary. Advanced PCR instruments cannot be popularized and applied, which not only complicates the production process, but also reduces the repeatability of experiments, so that conventional solid-phase PCR can only complete scientific research work in a laboratory environment

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