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Detection kit for determining lipoprotein related phospholipase A2 by virtue of quantitative immunosuppression method and application

A detection kit, immunosuppressive technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to reflect the real content of Lp-PLA2, inaccurate detection results, etc., and achieve reliable and accurate repeatable experimental results. , the effect of accurate test results

Inactive Publication Date: 2017-06-20
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved in the present invention is: how to provide a detection kit and application of a quantitative immunosuppressive method for measuring lipoprotein-associated phospholipase A2, so that it has good stability and high test efficiency. , accurate test results, and high sensitivity can solve the existing technical problems of the existing Lp-PLA2 detection methods that are easily affected by external interference and cause inaccurate test results, or cannot reflect the true content of biologically active Lp-PLA2

Method used

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  • Detection kit for determining lipoprotein related phospholipase A2 by virtue of quantitative immunosuppression method and application

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Effect test

Embodiment 1

[0020] A detection kit for the determination of lipoprotein-associated phospholipase A2 by quantitative immunosuppressive method, which is composed of Lp-PLA2 R1 reagent and Lp-PLA R2 reagent; wherein, the Lp-PLA2 R1 reagent is obtained by the following method: weighing Potassium hydrogen 1.5 grams, disodium hydrogen phosphate 0.8 grams, proclin 300 20 uL, dissolved in 100 milliliters of purified water, made into LP-PLA2 R1 reagent; said Lp-PLA2 R2 reagent was obtained by the following method: weigh 1-tetradecanoyl -2-(4-nitrophenylsuccinyl) lecithin 0.25g, anti-human LP-PLA2 enzyme activity antibody 0.5mg, citric acid 0.03g, sodium 1-nonanesulfonate 0.04g, vitamin E 0.02g, proclin 300 20ul was dissolved in 100ml of purified water, and the solution was adjusted to pH 5.00 with 0.1M NaOH to make Lp-PLA2R2 reagent.

[0021] R1 reagent and R2 reagent are used together.

Embodiment 2

[0023] A detection kit for the determination of lipoprotein-associated phospholipase A2 by quantitative immunosuppressive method, which is composed of Lp-PLA2 R1 reagent and Lp-PLA R2 reagent; wherein, the Lp-PLA2 R1 reagent is obtained by the following method: weighing Potassium hydrogen 2.0 grams, disodium hydrogen phosphate 1.2 grams, proclin 300 20 uL, dissolved in 100 milliliters of purified water, made into LP-PLA2 R1 reagent; said Lp-PLA2 R2 reagent was obtained by the following method: weigh 1-tetradecanoyl -2-(4-nitrophenylsuccinyl) lecithin 0.25g, anti-human LP-PLA2 enzyme activity antibody 0.5mg, citric acid 0.03g, sodium 1-nonanesulfonate 0.04g, vitamin E 0.02g, proclin 300 20ul was dissolved in 100ml of purified water, and the solution was adjusted to pH 5.00 with 0.1M NaOH to make Lp-PLA2R2 reagent.

[0024] R1 reagent and R2 reagent are used together.

Embodiment 3

[0026] A detection kit for the determination of lipoprotein-associated phospholipase A2 by quantitative immunosuppressive method, which is composed of Lp-PLA2 R1 reagent and Lp-PLA R2 reagent; wherein, the Lp-PLA2 R1 reagent is obtained by the following method: weighing Potassium hydrogen 2.4 grams, disodium hydrogen phosphate 1.6 grams, proclin 300 20 uL, dissolved in 100 milliliters of purified water, was made into LP-PLA2 R1 reagent; said Lp-PLA2 R2 reagent was obtained by the following method: weigh 1-tetradecanoyl -2-(4-nitrophenylsuccinyl) lecithin 0.25g, anti-human LP-PLA2 enzyme activity antibody 0.5mg, citric acid 0.03g, sodium 1-nonanesulfonate 0.04g, vitamin E 0.02g, proclin 300 20ul was dissolved in 100ml of purified water, and the solution was adjusted to pH 5.00 with 0.1M NaOH to make Lp-PLA2R2 reagent.

[0027] R1 reagent and R2 reagent are used together.

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Abstract

The invention provides a detection kit for determining lipoprotein related phospholipase A2 by virtue of a quantitative immunosuppression method and application. The detection kit comprises an Lp-PLA2 R1 reagent and an Lp-PLA2 R2 reagent, wherein the Lp-PLA2 R1 reagent contains a buffer matter pair, a preservative and water; and the Lp-PLA2 R2 reagent contains buffer matters, an anti-human Lp-PLA2 enzyme activity antibody, 1-tetanoyl-2-(4-nitrophenyl succinyl)lecithin, 1-nonane sulfonate, vitamin E, a preservative and water. The detection kit can be applied to the detection of the content of lipoprotein related phospholipase A2 in a sample. The detection kit has the characteristics of good stability, high testing efficiency and sensitivity, and a test result is accurate.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection reagents, and in particular relates to a detection kit for measuring lipoprotein-associated phospholipase A2 by a quantitative immunosuppressive method and an application thereof. Background technique [0002] Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a member of the phospholipase A2 superfamily that has attracted widespread attention in recent years and is closely related to atherosclerosis and ischemic cardiovascular and cerebrovascular diseases. It can be used as a new Inflammation markers and independent risk factors for predicting cardiovascular and cerebrovascular diseases. [0003] At present, there are two detection methods of Lp-PLA2 in the prior art: enzyme biochemical assay and immunological quality assay; the former enzyme biochemical assay is established based on the catalytic substrate of Lp-PLA2 enzyme activity, and has the ability of immediate reaction of Lp-P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 尹连杰于建华
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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