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Method for analyzing and testing urea content in raw milk

A technology for urea content, analysis and testing, applied in the field of raw milk testing, can solve problems such as cumbersome operation and long time-consuming, and achieve the effects of simple testing operation, low environmental requirements, and low testing cost.

Inactive Publication Date: 2020-04-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a new method for detecting urea content in raw milk to solve the problems of long time consumption, cumbersome operation and professional technical personnel in the existing analysis and test method of urea content in raw milk

Method used

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  • Method for analyzing and testing urea content in raw milk
  • Method for analyzing and testing urea content in raw milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 This embodiment is carried out according to the following steps:

[0014] (1) Preparation of reaction reagents: Weigh 10.0 g of potassium hydrogen sulfate, dissolve it in deionized water and dilute to 100 mL and mix evenly; weigh 1.0 g of p-dimethylaminobenzaldehyde, dissolve it in acetone and dilute to 100 mL, mix Uniform; Weigh 0.1 g of potassium sodium tartrate, dissolve in deionized water and dilute to 100 mL and mix evenly; weigh 0.1 g ascorbic acid, dissolve in deionized water and dilute to 100 mL and mix evenly.

[0015] (2) Production and use of test paper: Soak medium-thickness acid-resistant absorbent paper in the 10% potassium bisulfate solution prepared in step (1) for 10 minutes, heat and dry in a drying oven at 65°C after completely soaking, and dry it for 20 minutes. Take it out after 10 minutes, and immediately soak the taken out absorbent paper in 1% p-dimethylaminobenzaldehyde solution for 20 minutes, then place it in a drying oven at 65°C...

Embodiment 2

[0016] Embodiment 2 This implementation mode is carried out according to the following steps:

[0017] (1) Preparation of reaction reagents: Weigh 10.0 g of potassium bisulfate, dissolve it in deionized water and dilute to 100 mL and mix evenly; weigh 2.0 g of p-dimethylaminobenzaldehyde, dissolve it in acetone and dilute to 100 mL, mix Uniform; Weigh 0.1 g of potassium sodium tartrate, dissolve in deionized water and dilute to 100 mL and mix evenly; weigh 0.1 g ascorbic acid, dissolve in deionized water and dilute to 100 mL and mix evenly.

[0018] (2) Production and use of test paper: Soak medium-thickness acid-resistant water-absorbing paper in the 10% potassium bisulfate solution prepared in step (1) for 10 minutes, heat and dry in a drying oven at 65°C after fully soaking, Take it out after 10 minutes, then immediately immerse the taken out absorbent paper in 2% p-dimethylaminobenzaldehyde solution for 20 minutes, place it in a drying oven at 65°C, and take it out after 1...

Embodiment 3

[0019] Embodiment 3 This implementation mode is carried out according to the following steps:

[0020] (1) Preparation of reaction reagents: Weigh 10.0 g of potassium hydrogen sulfate, dissolve it in deionized water and dilute to 100 mL and mix evenly; weigh 1.0 g of p-dimethylaminobenzaldehyde, dissolve it in acetone and dilute to 100 mL, mix Uniform; Weigh 0.1 g of potassium sodium tartrate, dissolve in deionized water and dilute to 100 mL and mix evenly; weigh 0.1 g ascorbic acid, dissolve in deionized water and dilute to 100 mL and mix evenly.

[0021] (2) Production and use of test paper: Soak medium-thickness acid-resistant absorbent paper in the 10% potassium bisulfate solution prepared in step (1) for 10 minutes, heat and dry in a drying oven at 65°C after completely soaking, and dry it for 20 minutes. Take it out after 10 minutes, and immediately soak the taken out absorbent paper in 1% p-dimethylaminobenzaldehyde solution for 20 minutes, then place it in a drying ove...

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Abstract

The invention relates to the field of chemical detection, and particularly discloses test paper for rapidly detecting the content of urea and application of the test paper. A test paper soaking solution contains potassium thiocyanate, p-dimethylaminobenzaldehyde, tartrate and ascorbic acid. The detection wavelength of the test paper on the photoelectric color photometer is 420-450 nm, and the reaction time is 5-15 min. The method has the advantages of being sensitive in reaction, convenient to operate and the like, and detection accuracy and sensitivity are high.

Description

technical field [0001] The invention relates to the field of raw milk detection, in particular to a preparation method of a test paper for rapid detection of urea content in raw milk. Background technique [0002] Urea is the final product of protein metabolism and exists in the milk of mammals. In order to seek high profits, some unscrupulous merchants often mix urea in raw milk, which seriously affects the nutritional value of milk. Therefore, a simple, low-cost, and high-accuracy The detection method is to detect the urea content in the milk. At present, the detection methods of urea content in raw milk used in the dairy industry at home and abroad include nitrite-Griess reagent method and dimethylglyoxime photometry. Among them, the nitrite-Griess reagent method takes a long time to react; and the dimethylglyoxime photometric method is cumbersome to operate and requires professional technicians to operate. Contents of the invention [0003] The object of the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/50
CPCG01N21/78G01N33/50G01N2021/7759
Inventor 马芸田然马志刘卜玮谷眉宇田波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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