Method for quantitative detection of DNA using quantum dot as fluorescence tracer

A fluorescent tracer, quantitative detection technology, applied in the field of quantitative detection of DNA, to achieve the effect of a simple DNA quantitative detection method
CN1876838AInactive Publication Date: 2006-12-13SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2006-12-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

A quantitative determination method for DNA with quantum point as fluorescent tracer belongs to the DNA detecting domain. The invention builds the standard detection curve making use of that the quantum point has excellent fluorescent characteristic, DNA can quench the fluorescent signal of the quantum point and the signal quenched degree and the DNA quantity construct positive correlation, and obtains the DNA concentration of the solution using the fluorescent intensity and the standard curve of the quantum point in the measuring solution; the signal quenched degree and the DNA quantity construct positive correlation and the specific numerical relationship is: DNA concentration equals to 10(1264.4-Y) / 1786ng / mL; the DNA concentration of the solution can be calculated using the fluorescent intensity and the standard curve of the quantum point in the measuring solution. The invention can be used in calculation of fluorescence labeling DNA quantity, and the method is simple, fast and sensitive.
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Description

technical field

[0001] The invention relates to a method in the technical field of DNA detection, in particular to a method for quantitatively detecting DNA using quantum dots as fluorescent tracers. Background technique

[0002] The traditional method for quantitative detection of DNA is spectrophotometry, which detects the absorbance value at 260 nanometers, and the DNA content = OD 260 ×50μg / ml, this method is more complicated to operate; another common method is to use ethidium bromide combined with DNA molecules to generate red fluorescence under the excitation of ultraviolet light. The fluorescence intensity is proportional to the amount of DNA. Using the standard curve, DNA Quantitative analysis, because ethidium bromide is a carcinogen, should not be promoted. Currently, TaqMan probes are used for fluorescent quantitative PCR detection, but the cost is high and the background signal is strong, which has a certain impact on a wide range of quantitative detection appl...

Claims

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