77 results about "Fluorescent tracer" patented technology

The invention discloses a concrete test crack observation method based on a fluorescence tracing technology, and relates to the technical field of civil engineering research, and the concrete test crack observation method comprises the following specific steps: S100, building a concrete crack tracking detection device, S200, coating the surface of a concrete test piece with a fluorescence tracer agent, S300, collecting a fluorescence image, and S400, calculating a quantitative parameter. S500, establishing dynamic correlation between crack evolution and mechanical parameter variation, the crack development condition in the concrete uniaxial and splitting test process is directly observed by using a fluorescent agent and an ultraviolet lamp in the concrete uniaxial and splitting test process, a stress-strain curve in the concrete is recorded in real time through a computer in the mechanical loading test process, and the dynamic correlation between the crack evolution and the mechanical parameter variation is established. A high-speed camera is adopted to capture a high-definition fluorescence image of concrete surface crack propagation in real time, and the fluorescence image recorded in real time is quantified to obtain the development condition of concrete surface cracks, so that the relation between macroscopic strength such as uniaxial compression and microscopic cracks is established.

The invention relates to the technical field of medical instruments, and discloses a breast cancer sentinel lymph node positioning system and method, and the system comprises a data collection subsystem, a space positioning subsystem and a central processing subsystem. The method comprises the following steps: acquiring an anatomical structure image, a radioactivity counting rate and fluorescence intensity after performing primary fluorescence, radioactivity and secondary pulse type fluorescence tracer injection in sequence; establishing a global coordinate system and generating an anatomical region of interest; constructing a weighted directed topological graph based on the initial fluorescence data, and obtaining a baseline flow velocity; extracting radionuclide dynamic characteristics based on the radioactive data; a functional flow rate attenuation coefficient is calculated based on the second pulsed fluorescence data. And a multi-factor scoring model is applied, topological information, dynamic characteristics and functional flow velocity attenuation coefficients are fused, and a comprehensive score is calculated to identify the sentinel lymph node. According to the method, the lymphatic flow topological structure, nuclide dynamics and functional flow velocity information are combined, and the positioning accuracy and reliability are improved.

The invention discloses a method and a system for detecting an antibacterial coating micro guide wire. The method comprises the following steps: applying a composite stress field to a guide wire to obtain stress distribution data; determining a stress response abnormal area according to the stress distribution data and applying dual-frequency vibration excitation to obtain nonlinear vibration response data and vibration energy dissipation data; force-sensitive fluorescent molecules are applied to the stress response abnormal area for treatment, and conformational changes of the stress response abnormal area are monitored to obtain preliminary spatial distribution data of the microcracks; injecting a multi-ion fluorescent tracer into the micro-crack area, and monitoring the migration rule of the multi-ion fluorescent tracer to obtain pH value distribution data and oxidation-reduction potential distribution data; bacterial suspension with specific fluorescence response is injected into the micro-crack area, and antibacterial activity evaluation data is obtained by monitoring the fluorescence response of the bacterial suspension. According to the technical scheme, the microcracks on the surface of the antibacterial coating micro guide wire are accurately detected and positioned, and the influence degree of the microcracks on the antibacterial function can be accurately evaluated.

A method for non-invasively monitoring fluorescent tracers with background separation correction is disclosed, particularly a method for monitoring time-varying fluorescence emitted from a fluorescent agent within a diffusely reflecting medium having time-varying optical properties. The method comprises providing at least two measurements obtained from a patient before and after administration of the fluorescent agent, the measurements comprising a Flr adjacent to the medium detected by a filtered light detector during illumination of the medium with light of an excitation wavelength. meas signal, and at least one DR signal, the DR signal is selected from: #imgabs0#DR em and DR em,filtered The method further includes identifying a post-balanced portion of the measurement data set and assigning each Flr within the post-balanced portion of the measurement data set meas Signal conversion to IF agent signal, the IF agent The signal represents the intensity of the detected fluorescence emitted only by the fluorescent agent in the medium. The disclosed method includes eliminating the effects of leakage of excitation level light and meas Eliminate the influence of autofluorescence in the signal.

The present invention relates to the field of tracer technology, and discloses a codable and decodable magnetic fluorescent tracer, comprising a magnetic fluorescent microsphere comprising a core formed by a plurality of fluorescent materials, a polymer coating the core, and a plurality of magnetic microspheres embedded on the surface of the polymer. The tracer has both magnetic and fluorescent functions. When sampling and testing production wells, the tracer can be enriched using a magnetic field, reducing the difficulty of purification from the produced fluid. Compared with direct testing of the original fluid, the error of subsequent analysis and detection after enrichment of the tracer is significantly reduced. The tracer can be prepared in more than 20 different colors and can be decoded using a flow cytometer, increasing the variety of fluorescent tracers.

The utility model relates to the technical field of sports safety, in particular to an anti-drowning armlet which comprises a wearing assembly, an inflation assembly, a monitoring assembly and an early warning assembly. A monitoring assembly for monitoring the motion condition of a user and the surrounding environment is installed on the side, close to the user, in the wearing assembly, and an early warning assembly for early warning is installed on the surface of the outer side of the wearing assembly. Through mutual cooperation of the wearing assembly, the inflation assembly, the monitoring assembly and the early warning assembly, when too many factors possibly causing drowning exist in the swimming process of a user, the user can keep away from the drowning factors by himself or herself or call others to help keep away from the drowning factors through early safety warning, drowning events are avoided as much as possible, and the safety of the user is improved. In addition, once an actual drowning event occurs, the air bag can be automatically popped up to emerge from the water surface, and the fluorescent tracer agent is used for tracing the drowning event to a rescued person to find the drowning event.

The invention discloses an asphalt and aggregate interface action mechanism research method and system based on a multi-scale fluorescence tracer, and the method comprises the steps: collecting surface fluorescence images of asphalt and aggregate, carrying out the gray processing of the surface fluorescence images, and respectively calculating the average gray value of the asphalt surface and the average gray value of the aggregate surface; the method comprises the following steps: respectively carrying out infrared spectrum detection at an initial stage and a final stage of fluorescence tracing of asphalt to obtain dynamic change data of functional groups and chemical bonds in different stages, and establishing a corresponding relationship between fluorescence intensity attenuation and chemical bond evolution; according to the model, a fluorescent tracer layer structure model is constructed to obtain motion trail data of the fluorescent tracer on the asphalt and aggregate interface, the composition of the interface energy delta E1 of the fluorescent tracer and the asphalt and the interface energy delta E2 of the fluorescent tracer and the aggregate is determined, and the interface interaction characteristics of the fluorescent tracer, the asphalt and the aggregate are represented according to the numerical difference between the delta E1 and the delta E2. The problem that it is difficult to analyze the action mechanism and fluorescence decay of the road fluorescent tracer from a multi-scale level is solved.

The invention discloses a flow measurement device and method based on a fluorescent tracer time-difference method, and relates to the technical field of flow measurement based on the fluorescent tracer time-difference method, and the device comprises a tracer injection unit which comprises a tracer liquid storage cavity, a control pump / valve and an injection hole; the double detection units comprise a first fluorescence detection unit, a second fluorescence detection unit, a signal processing and control unit and a pressure liquid level unit. The first fluorescence detection unit and the second fluorescence detection unit are located on the two sides of the axial distance of the injection hole, and the distance is L. According to the tracer agent release time T0, the fluorescence signal appearance time t1 and t2, the high-concentration wave peak time T1 and T2 and the fluorescence signal curve, the water flow direction is judged, the flow speed is calculated, and meanwhile the flow is measured according to the liquid level data of the pressure liquid level unit.

The application provides a fluorescent tracing method and an aqueous zinc ion battery, wherein the fluorescent tracing method comprises the following steps: firstly, a fluorescent electrolyte solution is prepared, the fluorescent electrolyte solution contains a TPE-based fluorescent tracer, the TPE-based fluorescent tracer is a TPE derivative formed by introducing different functional groups into a TPE molecular skeleton, and the TPE derivative comprises an olefin group and a polar group; and secondly, the aqueous zinc ion battery containing the fluorescent electrolyte solution is subjected to a constant current deposition stripping mode to perform long-term cycle testing on the battery. In the long-term cycle testing of the battery, the tetraphenyl ethylene derivative with the aggregation-induced emission characteristic is introduced, the zinc ion migration, deposition and stripping behavior can be directly observed by means of the fluorescent signal of the tetraphenyl ethylene derivative, and the zinc element distribution and dynamic change are directly presented. This breaks through the limitation that the traditional method is difficult to directly capture the zinc electrode interface reaction, and provides intuitive visual evidence for understanding the electrochemical mechanism of the zinc ion battery.

This invention provides a tracer-type compound scale inhibitor based on benzofuran fluorescent agents, its preparation method, and its application. In the tracer-type compound scale inhibitor, the mass ratio of scale inhibitor to benzofuran fluorescent agent is (20-100):1; the benzofuran fluorescent agent is [6-hydroxy-2-(2-hydroxy-4-oxocyclohexyl-2,5-diene-1-yl)benzofuran-3-one]; the scale inhibitor is selected from acrylic acid-2-acrylamide-2-methylpropanesulfonic acid copolymer and / or ethylenediaminetetramethylene phosphate sodium. The benzofuran fluorescent agent of this invention combines real-time concentration detection and scale inhibition performance, exploring the potential of fluorescent tracers in water treatment. Due to the synergistic effect between BD and PAA, the tracer-type compound scale inhibitor prepared by this invention exhibits good fluorescence stability and excellent calcium carbonate scale inhibition rate, allowing for widespread application in circulating cooling water systems and other fields with relatively low dosage.

The invention discloses a wafer cleaning nozzle efficiency quantitative characterization method and system based on a fluorescent tracing imaging technology, and belongs to the technical field of semiconductor cleaning. The method comprises the following steps: uniformly mixing a pollutant standard solution with controllable viscosity and a fluorescent tracer agent to form a pollutant simulation solution capable of being quantitatively traced; spin-coating quantitative pollutant simulation liquid on the surface of the test substrate based on spin-coating equipment to form a uniform pollution layer; a to-be-evaluated cleaning nozzle is adopted to carry out directional cleaning on a preset polluted substrate, a fluorescence tracer is excited through an excitation light source, and a fluorescence signal distribution image in the cleaning process is collected through a charge-coupled device camera imaging system; the pollutant residual rate is accurately calculated through an image processing algorithm, and a quantitative evaluation index of the nozzle cleaning efficiency is established. According to the invention, visual monitoring of the whole cleaning process and accurate quantitative evaluation of the cleaning efficiency are realized, and a scientific quantitative basis is provided for parameter optimization and process verification of high-precision cleaning equipment.

The invention provides a joint detection system and method for leakage of a carbon dioxide storage tank, a terminal and a medium, a sensor network is constructed to cover a monitoring area, and the system comprises gas flow sensors arranged at an outlet of the storage tank and a conveying pipeline, and a carbon dioxide concentration sensor and a fluorescence detection sensor which are arranged at key points around the storage tank and the pipeline. And a trace fluorescent tracer agent is attached to the monitoring area. Leakage is preliminarily judged according to data collected by a gas flow sensor, if leakage occurs, the data collected by a carbon dioxide sensor and a fluorescence sensor are utilized, a Gaussian diffusion model and a fluid dynamic model are combined, the change direction of fluorescence intensity and carbon dioxide concentration is calculated, a leakage point is accurately positioned, and the leakage amount is calculated. The leakage severity is judged according to the gas flow and the carbon dioxide concentration, an alarm is triggered, and the leakage point position and the leakage amount are transmitted to a control center. The system integrates the fluorescent tracer and various sensors, realizes real-time accurate positioning of leakage points, and has the advantages of high sensitivity, fast response, strong applicability and the like.

The present invention relates to the technical field of pulmonary vascular function analysis methods, and specifically to a pulmonary ICG fluorescence dynamic diffusion quantitative analysis method, comprising the following steps: acquiring a patient's lung slice image; receiving fluorescent tracer injection information; constructing a three-dimensional lung model based on the lung slice image; establishing a blood vessel model and a blood flow model based on the three-dimensional lung model; simulating the fluorescent tracer diffusion process in the three-dimensional lung model and the blood vessel model according to the fluorescent tracer injection information; calculating the flow rate of all pipelines using an optical flow method; determining key time points according to preset conditions; calculating the flow value of each pipeline at the key time point of the tracing process based on the key time points; calculating the maximum flow change rate and the maximum flow difference according to the flow values; acquiring physiological parameter information corresponding to the three-dimensional lung model, and generating a vascular function evaluation model, thereby improving the accuracy of blood vessel recognition, especially significantly improving the recognition ability of tiny blood vessels.

The invention discloses application of a clofazimine suspension as an NIR-II fluorescent tracer and a dye tracer in the field of auxiliary diagnosis and treatment. The preparation has the advantages of good biological safety (suitable for mothers and infants), high sensitivity, high tissue penetrability, light stability and the like, and can reduce the inconvenience of clinical multiple administration and improve the compliance of patients. The NIR-II fluorescence imaging capability overcomes the limitation of the traditional imaging in strong light or deep tissue'black box 'environment, and is more suitable for intraoperative navigation. Meanwhile, due to the inherent red dyeing characteristic, the dye can be used as a lymph dye tracer. In combination with the NIR fluorescence effect and the photoacoustic effect of the clofazimine suspension, the intraoperative lymphatic cleaning can be assisted through a multi-mode combined tracing technology.

This invention discloses a slope soil aggregate erosion and disintegration testing device, belonging to the field of geotechnical engineering testing technology. The device includes: a model box assembly, a rainfall simulation assembly, an optical observation assembly, and a runoff collection assembly. The model box assembly includes a box body and a slope adjustment mechanism. The slope adjustment mechanism uses multiple hinged plates in conjunction with a hydraulic drive device to achieve precise adjustment of the slope angle in segments. The uppermost layer of the filling area inside the box body is divided into several tracer zones, each tracer zone being covered with a soil layer containing different colored fluorescent tracers. The rainfall simulation assembly simulates natural rainfall processes. The optical observation assembly includes a light shield, an ultraviolet light source, a camera, and a filtering system to achieve fluorescence imaging. The runoff collection assembly uses a sampler to collect sediment samples at different time intervals. This invention can accurately track the soil loss path at different slope locations, enabling visualized observation and quantitative analysis of the soil erosion process, providing a systematic experimental method for studying soil disintegration mechanisms.

This invention relates to a method for visually detecting the interface of a welded joint in thermoplastic composite materials, comprising the following steps: placing a fluorescent tracer film at the interface of two thermoplastic composite components, such that the film covers at least a portion of the interface area, wherein the fluorescent tracer film is a film formed by uniformly doping a thermoplastic resin compatible with the thermoplastic composite matrix resin with nano-sized phosphors; applying energy sufficient to cause local melting and flow of the matrix resin to the interface, causing the fluorescent tracer film and the matrix resin to melt and flow together, and forming a luminescent marker layer fused to the interface after cooling; performing fluorescence imaging on the components after the melt-flow treatment to obtain a fluorescence distribution map of the interface area; extracting luminescence characteristic parameters based on the fluorescence distribution map, and constructing a visual model of the interface.

The invention discloses a method and system for testing wear resistance of a washing machine standard sample, and relates to the field of washing machine performance tests.The method comprises the steps that a regular super-hydrophilic stripe pattern is constructed on the surface of standard washing cloth through a mask spraying technology, and a fluorescent tracer sensitive to mechanical wear is evenly loaded on fibers through an impregnation method; a patterned fluorescence function standard sample is obtained; initially weighing the patterned fluorescence function standard sample, and respectively acquiring an initial appearance image and an initial fluorescence image under a standard light source and ultraviolet light; mixing the patterned fluorescent function standard sample with the standardized simulation piece to form a mixed load; and putting the mixed load into the washing machine for standard washing. The method provides more comprehensive data support for the design optimization of the washing machine by quantifying the function decline and microscopic damage of the fabric, improves the authenticity simulation of the test load, and solves the technical problem that the physical wear and the function characteristic decline cannot be evaluated at the same time in the prior art.

The utility model discloses a kind of foundation pit soil layering permeation and seepage visualization indoor model test box, belong to geotechnical engineering test equipment technical field, comprising: test box main body, multiple layering partition plates are provided in test box main body;Test box main body has at least one transparent front wall;Multiple permeation channels are provided on each layering partition plate, and the channel mouth of permeation channel is provided with electric opening and closing valve;Fluorescent tracer injection system cooperates with the fluorescent tracer injection hole of being set on the side plate of test box main body;Ultraviolet developing module is set with the front wall of test box main body with inclination angle;High-speed camera recording system is set corresponding with the front wall of test box main body;Multi-channel independent water pressure control unit is located at the side of test box main body away from fluorescent tracer injection system;Data acquisition and analysis module is electrically connected with high-speed camera recording system.The utility model can directly reveal piping, seepage erosion and other seepage failure mechanism, provide scientific basis for seepage prevention design optimization.

The invention discloses lipid molecules containing a near-infrared fluorescent tracer agent and application of the lipid molecules, and belongs to the field of fine chemical engineering. According to the series of molecules, near-infrared fluorescent dye with a fluorescent tracing effect is connected with lipid molecules, and the series of molecules belong to a class of novel bifunctional material molecules. The molecule contains a lipid side chain, so that the lipid molecule has the performance that the lipid molecule is combined with RNA / DNA and other base sequences and is self-assembled into nanoparticles, and the lipid molecule becomes a high-quality RNA / DNA delivery carrier material; and the fluorescent dye part shows strong NIR fluorescence, and the emission wavelength can reach 710 nm. Excellent imaging performance can be obtained in three cell line models: Hela, A549 and Raw cells. The whole process change of endosome escape of various cells can be traced with an accurate and good imaging effect, and an effective result associated with mRNA delivery and protein expression can be provided. We predict that we new methods would contribute to the development and optimization of various mRNA delivery vectors.

The application provides a functional temporary plugging material and a preparation method and application thereof. The functional temporary plugging material comprises a degradable matrix and fluorescent tracers as tracer parts; wherein, when the degradable matrix is 100 parts by weight, the amount of the fluorescent tracers is 0.05-5 parts by weight, preferably 0.1-1 parts by weight; preferably, the fluorescent tracers are at least 3 kinds. The degradation degree of the temporary plugging material can be understood by detecting the concentration of the fluorescent tracers in the formation water brought to the ground, the application can effectively eliminate interference factors, obtain a detection result with higher reliability and anti-interference ability, and can assist oilfield fracturing construction.

The invention discloses a shale wettability pore space characterization method, and relates to the field of shale pore analysis, and the method comprises the following steps: obtaining a shale sample; sequentially immersing the shale sample in a fat-soluble fluorescent tracer solution and a water-soluble fluorescent tracer solution; performing laser confocal test analysis on the immersed shale sample to obtain three-dimensional visual models of pore spaces with different wettability; determining a fluorescence scanning area volume, an oil wetting pore volume and a water wetting pore volume by utilizing image processing software according to the three-dimensional visual model; determining the wettability characteristic of the pore space of the shale sample according to the volume of the fluorescence scanning area, the volume of the oil-wetted pore and the volume of the water-wetted pore; the wettability characteristic of the pore space of the shale sample is water wettability or oil wettability. According to the invention, visualization and quantitative characterization of pore spaces with different wettability of shale can be realized at the same time.

The invention relates to the technical field of groundwater remediation, in particular to a circulating well-multiphase split-flow treatment system and method for groundwater remediation, and the system comprises a gas stripping unit, a circulating unit and a control unit; the method comprises the steps that the actual repairing range of the circulating well is determined according to the reduction amplitude of the diffusion rate of the fluorescent tracer agent; under the condition that horizontal deviation exists between the actual repairing range and the ideal repairing range, the gas injection amount of a circulating well is adjusted based on the judgment result that airflow escape exists, or the injection amount of a surfactant is adjusted based on the judgment result that the repairing range of the circulating well does not cover the low-permeability area, or the injection amount of the surfactant is adjusted based on the judgment result that the repairing range of the circulating well does not cover the low-permeability area. Under the condition that vertical deviation exists between the actual restoration range and the ideal restoration range, the gas injection temperature is adjusted based on the type of the vertical permeation barrier; and the actual concentration decline rate of the adjusted pollutants is obtained so as to optimize the actual restoration range. The remediation efficiency of underground water is improved.

The invention provides sericin microspheres as well as a preparation method and application thereof, and relates to the technical field of soft substance processing and microfluidics. The preparation method comprises the following steps: (1) taking an aqueous solution containing sericin as an aqueous phase 1; taking water or an aqueous solution containing auxiliary crosslinking components as an aqueous phase 2; (2) introducing the oil phase, the water phase 1 and the water phase 2 into a double-tube injection capillary glass tube microfluidic device, and shearing the water phase 1 and the water phase 2 by using the oil phase to prepare water-in-oil micro-droplets containing sericin; and (3) curing the micro-droplets containing the sericin protein to obtain the sericin protein microspheres. The microspheres are prepared from sericin as a raw material, and the prepared sericin microspheres are uniform in particle size and good in monodispersity, have rich autofluorescence characteristics, can be used as a fluorescent tracing carrier or an imaging material or used for carrying various active substances, omit an exogenous dye loading step, reduce toxicity and have good application prospects.

The invention discloses a concrete-template interface electro-osmosis membrane dynamic monitoring system and a testing method. The system comprises an electro-osmosis device, a water membrane monitoring system, a plastic rectangular groove, a potential acquisition device and a humidity acquisition device, wherein fresh concrete containing a fluorescent tracer agent is poured in the plastic rectangular groove, and the potential acquisition device and the humidity acquisition device are connected into the plastic rectangular groove. The method comprises the following steps: performing electroosmosis on fresh concrete containing a fluorescent tracer agent through an electroosmosis device, and recording humidity data and potential data at different positions in a plastic rectangular groove in an electroosmosis process by a humidity acquisition device and a potential acquisition device respectively; the water film monitoring system records the development process of forming the electro-permeable membrane in the cathode transparent electrode template in real time, and obtains the characteristic information of the water film area, the water film area change rate, the expansion speed and the thickness distribution of the electro-permeable membrane, so that quantitative research on the evolution law of the electro-permeable membrane is realized.

The application designs a series of xanthene near-infrared mitochondrial fluorescent tracers, the self-assembly characteristics of the dyes are regulated by the length of the external hydrophobic carbon chain, and then the stability of the mitochondrial targeting and the signal-to-noise ratio of the imaging are optimized. The fluorescent tracers show strong NIR fluorescence, the emission wavelength can reach 700 nm, the molar extinction coefficient is 10.9 M ‑1 *cm ‑1 *10 4 , the fluorescence quantum yield is 0.64. In the aqueous phase, the ACQ effect is significant, but it can be quickly enriched and embedded into mitochondria, and then the strong fluorescence is restored, showing high signal-to-noise ratio; in living cells, it has stable mitochondrial targeting, is not affected by the membrane potential, and the imaging performance is better than that of the commercial probe TMRM. The fluorescent tracer can accurately and stably trace the microtubular morphological changes of mitochondria in ferroptosis.

This application discloses a method for characterizing the wettability pore space of shale, relating to the field of shale porosity analysis. The method includes acquiring a shale sample; sequentially immersing the shale sample in a lipid-soluble fluorescent tracer solution and a water-soluble fluorescent tracer solution; performing laser confocal microscopy analysis on the immersed shale sample to obtain a three-dimensional visualization model of pore spaces with different wettability; determining the volume of the fluorescence scanning region, the volume of oil-wetted pores, and the volume of water-wetted pores using image processing software based on the three-dimensional visualization model; determining the wettability characteristics of the shale sample's pore space based on the fluorescence scanning region volume, the volume of oil-wetted pores, and the volume of water-wetted pores; wherein the wettability characteristics of the shale sample's pore space are water-wettable or oil-wettable. This application can simultaneously achieve visualization and quantitative characterization of pore spaces with different wettability in shale.

The invention relates to the technical field of transformer air tightness detection, in particular to a leakage testing liquid for transformer oil tank air tightness detection and a detection method. The leakage testing liquid is designed according to a composite formula and comprises perfluoropolyether oil serving as an inert carrier, a microencapsulation foaming agent for enhancing the bubble stability through a composite shell structure, a liquid crystal surfactant for improving the dispersion homogeneity, and a fluorescent tracer agent for realizing leakage visualization. The preparation process comprises the following steps: firstly prefabricating the microcapsule containing the nano reinforced wall material, then performing hot shearing to form a basic thixotropic system, and finally performing ultrasonic-assisted functional component integration to ensure that the product is homogeneous and stable for a long time. According to the detection method, real working conditions are simulated through variable-voltage pulse pressurizing, and ultraviolet light excites fluorescence after leakage testing liquid is sprayed; bubble rupture sound signals and fluorescence images are synchronously collected, space-time signals are correlated by using a cross correlation algorithm, and the coordinates of leakage points are accurately positioned. According to the method, the detection period is remarkably shortened, and the problems that traditional leakage testing liquid is rapid in sedimentation, weak in bubble response and fuzzy in leakage point positioning are solved.

The invention provides a soil aggregate stability analysis method and system, and relates to the technical field of soil quality evaluation.The method includes the steps that after drip irrigation water added with a fluorescent tracer agent is adopted for irrigating soil in a root zone under a film, dry screening is conducted on the soil, and an aggregate with the fluorescent tracer agent after dry screening is obtained and recorded as a target aggregate; loading the mixed fertilizer liquid into the target aggregate round by round, obtaining a permeation signal of the target aggregate under each loading round, determining a stage of the target aggregate under each loading round based on the permeation signal, and setting an instability condition for each type of target aggregate; and continuously loading the target aggregate until the instability condition is met, deriving the loading round times when the target aggregate is unstable, comparing the loading round times with the preset life loading round times, and judging that the stability of the target aggregate is qualified if the loading round times when the target aggregate is unstable are greater than the preset life loading round times.

The invention relates to the technical field of underground engineering grouting, in particular to a flowing water grouting plugging performance quantitative evaluation method based on micro-fluidic fluorescence imaging, which comprises the following steps: calibrating grout with different known concentrations by using a fluorescent tracer agent to establish a quantitative relation model; carrying out a flowing water grouting test in the micro-fluidic chip so as to capture a fluorescence image sequence in the slurry migration and plugging process; inverting the fluorescence image sequence into a slurry concentration spatio-temporal evolution field based on a quantitative relation model; identifying the slurry-flowing water interface in the spatio-temporal evolution field of the slurry concentration, and tracking the dynamic evolution process of the slurry-flowing water interface to calculate the formation time of the slurry-flowing water interface and the spatio-temporal evolution law of the slurry concentration; and calculating a plugging strength index according to the slurry concentration spatio-temporal evolution field and the slurry-flowing water interface forming time, and evaluating the plugging efficiency of the grouting material by using the plugging strength index. Therefore, the problems that in an existing flowing water grouting technology, the plugging process is invisible, and key performance is difficult to quantify are solved.

The invention belongs to the technical field of protein detection, and provides an amyloid transthyretin detection kit and application thereof, the kit comprises the following reagents: normal recombinant TTR protein, a TTR-PFF standard substance, a fluorescent tracer and a buffer solution system; the TTR-PFF standard substance comprises a TTR substrate and an RT-QuIC reaction mixed solution, and the pH value of the RT-QuIC reaction mixed solution is 4.0 to 4.5; the buffer solution system comprises a PBS / N2 sample dilution buffer solution and a phosphate buffer solution. According to the detection kit, TTR-PFF is adopted as a reference, a subject sample is combined with normal recombinant human transthyretin, a fluorescent tracer is added, ATTR in a body can convert normal recombinant monomer TTR protein into amyloid fiber, and existence and activity of the ATTR are detected by detecting a fluorescence value of the ATTR. The detection has ultrahigh sensitivity and specificity, minimally invasive or non-invasive sampling is operated, the operation is simple and convenient, a key technical foundation is laid for developing a simple, convenient, efficient and clinical ATTR amyloidosis detection platform, especially an early detection platform, and the kit has a wide market application prospect.