A latex-enhanced immunoturbidimetric kit for quantitative detection of c-peptide

An immunoturbidimetric and latex-enhanced technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unstable test results, high equipment requirements, and low degree of automation, so as to improve accuracy and credibility , reduce production costs, and achieve a high degree of automation
CN103852584BActive Publication Date: 2016-04-13CHONGQING ZHONGYUAN BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
CHONGQING ZHONGYUAN BIOLOGICAL TECH
Publication Date
2016-04-13

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Abstract

The invention discloses a latex immune enhancement turbidimetric kit for detecting peptide C quantitatively. The latex immune enhancement turbidimetric kit for detecting peptide C quantitatively consists of a peptide C R1 reagent, a peptide C R2 reagent and a peptide C calibration product, wherein the peptide C R1 reagent comprises a buffer liquid, a protective agent I, a reaction enhancer and preservatives; the peptide C R2 reagent comprises a buffer liquid, a protective agent I, preservatives and sensitization polystyrene latex particles coated with anti-human peptide C antibodies; the peptide C calibration product comprises a buffer liquid, a protective agent II, preservatives and peptide C recombinant protein. The kit prepared by the invention is suitable for semi-automatic and full-automatic biochemical analyzers and scattering turbidimetric analyzers, has the advantages of simple and rapid operation, high testing accuracy and high automation degree, is suitable for being used clinically and widely, and is especially suitable for rapid and quantitative determination of emergency treatment.
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Description

technical field

[0001] The invention belongs to the field of in vitro diagnostic reagents, and in particular relates to a kit for quantitatively measuring and detecting C-peptide content in specimens by using latex-enhanced immune turbidimetry. Background technique

[0002] C-peptide, also known as connecting peptide, is composed of 31 amino acids and has a molecular weight of 3020 Daltons. It is a short peptide connecting insulin A chain and B chain in proinsulin, and its function is to promote insulin molecules in ฮฒ cells. Synthesized in the endoplasmic reticulum and enables efficient folding and assembly of insulin. Clinically, the determination of C-peptide is mainly used to correctly evaluate the functional changes and development rules of islet ฮฒ cells, as well as to accurately determine the type of diabetes and monitor the condition.

[0003] Human pancreatic ฮฒ-cells synthesize proinsulin, which is subsequently cleaved equimolarly into insulin and C-peptide. Therefo...

Claims

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