A latex-enhanced immunoturbidimetric kit for quantitative detection of c-peptide

An immunoturbidimetric and latex-enhanced technology, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unstable test results, high equipment requirements, and low degree of automation, so as to improve accuracy and credibility , reduce production costs, and achieve a high degree of automation

Active Publication Date: 2016-04-13
CHONGQING ZHONGYUAN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, clinical methods for the determination of C-peptide mainly include radioimmunoassay (RIA), enzyme-linked immunosorbent assay (Enzyme-Linked, ImmunosorbentAssay, ELISA) and chemiluminescence. High requirements on equipment, unstable test results and radioactive contamination; ELISA has poor quantitative accuracy, long operation time, and low degree of automation, which cannot meet the needs of a large number of rapid tests in clinical practice; the chemiluminescence method has expensive instrument maintenance and is susceptible to interference , the disadvantage of poor repeatability

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  • A latex-enhanced immunoturbidimetric kit for quantitative detection of c-peptide
  • A latex-enhanced immunoturbidimetric kit for quantitative detection of c-peptide
  • A latex-enhanced immunoturbidimetric kit for quantitative detection of c-peptide

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Embodiment 1

[0038] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0039] Taking the preparation of 1LC peptide R1 reagent as an example, it is prepared from Tris-HCl buffer solution with a concentration of 50mM, 3g bovine serum albumin BSA, 1g sodium azide and 50g PEG6000, and the concentration of Tris-HCl buffer solution in the final C-peptide R1 reagent is 50mM, the concentration of bovine serum albumin BSA is 3g / L, the concentration of sodium azide is 1g / L, and the concentration of PEG6000 is 50g / L;

[0040] Taking the preparation of 1LC peptide R2 reagent as an example, it is prepared from Tris-HCl buffer solution with a concentration of 50mM, 5g of bovine serum albumin BSA, 1g of sodium azide and sensitized polystyrene latex particles coated with anti-human C-peptide antibody Obtained, the concentration of Tris-HCl buffer solution in the final C peptide R2 reagent is 50...

Embodiment 2

[0056] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0057] Taking the preparation of 1LC peptide R1 reagent as an example, it is prepared from 200mM glycine buffer, 5g bovine serum albumin BSA, 2g sodium azide and 40g PEG8000. The concentration of glycine buffer in the final C peptide R1 reagent is 200mM, bovine serum albumin The concentration of protein BSA is 5g / L, the concentration of sodium azide is 2g / L, and the concentration of PEG8000 is 40g / L;

[0058] Taking the preparation of 1LC peptide R2 reagent as an example, it is prepared from 50mM borax buffer solution, 5g bovine serum albumin BSA, 0.3g sodium azide and sensitized polystyrene latex particles coated with anti-human C-peptide antibody , the concentration of borax buffer in the final C-peptide R2 reagent is 50mM, the concentration of bovine serum albumin BSA is 5g / L, and the concentration of sodiu...

Embodiment 3

[0062] A latex-enhanced immunoturbidimetric kit for quantitative detection of C-peptide, including C-peptide R1 reagent, C-peptide R2 reagent and C-peptide calibrator.

[0063] Taking the preparation of 1LC peptide R1 reagent as an example, it is prepared from PBS buffer solution with a concentration of 200mM, 5g bovine serum albumin BSA, 3g sodium azide and 50g PEG8000, the final concentration of PBS buffer solution in the C peptide R1 reagent is 200mM, bovine serum The concentration of albumin BSA is 5g / L, the concentration of sodium azide is 3g / L, and the concentration of PEG8000 is 50g / L;

[0064] Taking the preparation of 1LC peptide R2 reagent as an example, it is prepared from borax buffer solution with a concentration of 50mM, 5g of bovine serum albumin BSA, 1g of sodium azide, and sensitized polystyrene latex particles coated with anti-human C-peptide antibody. The concentration of borax buffer in the final C-peptide R2 reagent is 50mM, the concentration of bovine ser...

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Abstract

The invention discloses a latex immune enhancement turbidimetric kit for detecting peptide C quantitatively. The latex immune enhancement turbidimetric kit for detecting peptide C quantitatively consists of a peptide C R1 reagent, a peptide C R2 reagent and a peptide C calibration product, wherein the peptide C R1 reagent comprises a buffer liquid, a protective agent I, a reaction enhancer and preservatives; the peptide C R2 reagent comprises a buffer liquid, a protective agent I, preservatives and sensitization polystyrene latex particles coated with anti-human peptide C antibodies; the peptide C calibration product comprises a buffer liquid, a protective agent II, preservatives and peptide C recombinant protein. The kit prepared by the invention is suitable for semi-automatic and full-automatic biochemical analyzers and scattering turbidimetric analyzers, has the advantages of simple and rapid operation, high testing accuracy and high automation degree, is suitable for being used clinically and widely, and is especially suitable for rapid and quantitative determination of emergency treatment.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, and in particular relates to a kit for quantitatively measuring and detecting C-peptide content in specimens by using latex-enhanced immune turbidimetry. Background technique [0002] C-peptide, also known as connecting peptide, is composed of 31 amino acids and has a molecular weight of 3020 Daltons. It is a short peptide connecting insulin A chain and B chain in proinsulin, and its function is to promote insulin molecules in β cells. Synthesized in the endoplasmic reticulum and enables efficient folding and assembly of insulin. Clinically, the determination of C-peptide is mainly used to correctly evaluate the functional changes and development rules of islet β cells, as well as to accurately determine the type of diabetes and monitor the condition. [0003] Human pancreatic β-cells synthesize proinsulin, which is subsequently cleaved equimolarly into insulin and C-peptide. Therefo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/545G01N33/6803G01N2333/62
Inventor 李民友吉权
Owner CHONGQING ZHONGYUAN BIOLOGICAL TECH
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