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Low-template-consumption punicalin imprinting method based on dual-crowding effect

A pomegranate grove and crowding technology, which is applied in the field of imprinting of pomegranate groves with low template consumption based on the double crowding effect, can solve the problems of high cost of imprinting, consumption of large pomegranate groves, and high price of pomegranate groves, achieving low cost of imprinting, Excellent retention performance, the effect of excellent retention performance

Inactive Publication Date: 2016-07-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of pomegranate groves is extremely expensive, and a large amount of pomegranate groves will be consumed during the imprinting process, so the cost of imprinting is relatively high

Method used

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  • Low-template-consumption punicalin imprinting method based on dual-crowding effect
  • Low-template-consumption punicalin imprinting method based on dual-crowding effect
  • Low-template-consumption punicalin imprinting method based on dual-crowding effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Two crowding reagents, polyethylene glycol with an average molecular mass of 6,000 and polystyrene with an average molecular mass of 350,000 were dissolved in tetrahydrofuran solution, and the concentration of polyethylene glycol in the obtained solution was 19.4 mg / mL, polystyrene The concentration is 40mg / mL, and the mass percentage of 0.035% pomegranate is dissolved in the tetrahydrofuran solution containing two crowding reagents with a mass fraction of 68.35%;

[0020] Then add acrylamide with a mass fraction of 2.48%, ethylene glycol dimethacrylate with a mass fraction of 20.80%, toluene with a mass fraction of 6.92%, and azobisisobutyronitrile with a mass fraction of 1.40%, and ultrasonically for 10 minutes. The solution was clarified, transferred to a stainless steel column (100×4.6mm) with a length of 100mm and a diameter of 4.6mm, sealed both ends of the stainless steel column tube, and thermally initiated the reaction in a constant temperature water bath at a t...

Embodiment 2

[0024] Preparation of Blank Control Column by In-Situ Polymerization

[0025] The blank control column was prepared by in-situ polymerization: two crowding reagents polyethylene glycol and polystyrene were dissolved in tetrahydrofuran solution respectively. mL;

[0026] Acrylamide with a mass percentage of 2.49%, ethylene glycol dimethacrylate with a mass fraction of 20.81%, toluene with a mass fraction of 6.93%, and azobisisobutyronitrile with a mass fraction of 1.40% were ultrasonically dissolved for 10 minutes. After the solution is clarified, transfer it to a stainless steel column (100×4.6mm), seal both ends of the stainless steel column tube, and thermally initiate the reaction in a constant temperature water bath at a temperature of 55°C for 24 hours;

[0027] Residual porogen and functional monomer removal: Take out the synthesized stainless steel column, put it on the column head, connect it to a high-pressure infusion pump, and use 150mL tetrahydrofuran, 100mL metha...

Embodiment 3

[0030] Evaluation of Selective Performance of Pomegranate Imprinted Monolithic Column and Its Blank Control Column

[0031] Selective performance evaluation of pomegranate imprinted monolithic column: set the UV absorption wavelength to 258nm, flow rate to 1.0mL / min, column temperature to 35°C, use acetonitrile-acetic acid buffer salt (pH3.6, 200mmol / L) (92:8, v / v) Flush the imprinted monolithic column of pomegranate to the baseline level, inject samples of pomegranate, punicalagin, ellagic acid and tannil extract respectively, and obtain the chromatograms of various substances on the monolithic column. Such as figure 2 It was shown that the imprinted monolithic column of punicalagin has a strong retention of punicalagin and punicalagin, and can effectively separate the two from ellagic acid and punicalagin. It shows that the present invention has the function of simultaneously separating punicalagin and punicalagin;

[0032] Selective performance evaluation of the blank c...

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Abstract

The invention relates to a low-template-consumption punicalin imprinting method based on the dual-crowding effect.According to the method, polystyrene and polyethylene glycol serve as two macromolecule crowding agents, an extremely small amount of punicalin serves as template molecules, a functional monomer and a cross-linking agent or initiator are added to a mixed pore-foaming agent formed by methylbenzene and a tetrahydrofuran solution containing the two crowding agents polystyrene and polyethylene glycol, and ultrasonic treatment is conducted to promote dissolution, remove oxygen dissolved in the mixture and induces reaction, so that a selective punicalin imprinted monolithic column is obtained.Template consumption is extremely low, and punicalin imprinting cost is reduced greatly.The punicalin imprinted monolithic column and a blank control column are successfully synthesized in a stainless steel column jacket, and chromatographic performance optimization is conducted.Results show that imprinting factor can be as high as 3.14. The imprinting method is simple, the durability of high-molecular polymers is high, and a low-cost method is provided for separation and purification of punicalin.

Description

technical field [0001] The invention relates to a low template consumption punicalagin imprinting method based on double crowding effect. The imprinted polymer exhibits good selectivity to punicalagin and its analogue punicalagin at the same time, and can be used for punicalagin and punicalagin Separation and purification of glycosides. Background technique [0002] Molecular imprinting is an emerging technology based on the "lock and key principle", which artificially synthesizes materials with specific selectivity by simulating the interaction between enzymes and substrates in biological cells. The target molecule (also known as "template") and functional monomers are dissolved in a certain porogen to self-assemble into a prepolymer, and then a crosslinking agent is added to crosslink under the trigger of light or heat to form a high porogen. molecular polymer. Washing the polymer with a highly polar solvent such as methanol-acetic acid releases the template from the pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/285B01D15/22B01D15/10G01N30/02
CPCB01J20/285B01D15/10B01D15/22G01N30/02
Inventor 刘照胜孙光映阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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