Application of artemisinin compound in treatment of coronavirus infection
Technology of a compound, artemisinin, applied in the field of biomedicine
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0134] Example 1: Artemisinin reduces the virus nucleic acid load experiment of SARS-CoV-2 infected cells
[0135] (1) Drug treatment of virus-infected cells
[0136] Inoculate Vero E6 cells (purchased from ATCC, Cat. No. 1586) into 24-well plates and culture for 24 hours; then carry out virus infection, specifically, with 2% cell maintenance solution (formulation: FBS (purchased from Gibco, Cat. No. 16000044) Add MEM (purchased from Gibco, product number 10370021) according to the volume ratio of 2%, which is 2% cell maintenance solution) SARS-CoV-2 (2019-nCoV) virus (nCoV-2019BetaCoV / Wuhan / WIV04 / 2019 strain, Provided by the Wuhan Institute of Virology, Chinese Academy of Sciences) diluted to the corresponding concentration, and then added to a 24-well plate so that each well contains 100 TCID of virus 50. Next, dilute artemisinin (purchased from Selleck Chemicals, product number S1282) to corresponding concentrations with 2% cell maintenance solution, and add to correspond...
Embodiment 2
[0175] Example 2: Artemisinin reduces the virus nucleic acid load experiment of SARS-CoV-2 infected cells
[0176] (1) Drug treatment of virus-infected cells
[0177] Inoculate Vero E6 cells (purchased from ATCC, Cat. No. 1586) into 24-well plates and culture for 24 hours; then carry out virus infection, specifically, with 2% cell maintenance solution (formulation: FBS (purchased from Gibco, Cat. No. 16000044) Add MEM (purchased from Gibco, product number 10370021) according to the volume ratio of 2%, which is 2% cell maintenance solution) SARS-CoV-2 (2019-nCoV) virus (nCoV-2019BetaCoV / Wuhan / WIV04 / 2019 strain, Provided by the Wuhan Institute of Virology, Chinese Academy of Sciences) diluted to the corresponding concentration, and then added to a 24-well plate so that each well contains 100 TCID of virus 50 . Next, artemisinin (purchased from MCE, product number HY-N2016) was diluted to corresponding concentrations with 2% cell maintenance solution, and added to corresponding...
Embodiment 3
[0216] Example 3: Artemether reduces the virus nucleic acid load experiment of SARS-CoV-2 infected cells
[0217] (1) Drug treatment of virus-infected cells
[0218] Inoculate Vero E6 cells (purchased from ATCC, Cat. No. 1586) into 24-well plates and culture for 24 hours; then carry out virus infection, specifically, with 2% cell maintenance solution (formulation: FBS (purchased from Gibco, Cat. No. 16000044) Add MEM (purchased from Gibco, product number 10370021) according to the volume ratio of 2%, which is 2% cell maintenance solution) SARS-CoV-2 (2019-nCoV) virus (nCoV-2019BetaCoV / Wuhan / WIV04 / 2019 strain, Provided by the Wuhan Institute of Virology, Chinese Academy of Sciences) diluted to the corresponding concentration, and then added to a 24-well plate so that each well contains 100 TCID of virus 50 . Next, artemether (purchased from Selleck Chemicals, product number S3889) was diluted to corresponding concentrations with 2% cell maintenance solution, and added to the ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com