Method for preparing artemisinic acid

A technology of artemisinic acid and Artemisia annua, applied in the biological field, can solve the problems of unsuitability for industrialized production, low extraction efficiency, complicated operation, etc., and achieves the effects of environmental friendliness, high extraction efficiency, and simple method operation.

Inactive Publication Date: 2013-12-25
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This type of method is complex in operation, low in extraction efficiency, and seriously polluted, and is not suitable for industrial production

Method used

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  • Method for preparing artemisinic acid

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Experimental program
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Effect test

Embodiment 1

[0016] Take 5kg of dried Artemisia annua raw material, pulverize it to 20 meshes, place it in a CO2 supercritical extraction tank for extraction, pass in liquid CO2, the flow rate is 3ml / min / g raw material, the extraction temperature is 40°C, the pressure is 18Mpa, and the extraction time is 2h. The extract was dissolved with 30 times the volume of 5% sodium bicarbonate solution. After the dissolved solution was filtered, an equal volume of petroleum ether was added and stirred thoroughly. The hydrochloric acid solution was adjusted to pH3. After 20 hours of crystallization, the crystals were recrystallized with anhydrous methanol and dried to obtain 3.2 g of artemisinic acid with a content of 98.6%.

Embodiment 2

[0018] Take 5kg of dried Artemisia annua raw material, pulverize it to 40 meshes, place it in a CO2 supercritical extraction tank for extraction, introduce liquid CO2, the flow rate is 5ml / min / g raw material, the extraction temperature is 45°C, the pressure is 20Mpa, and the extraction time is 1h. The extract was dissolved with 40 times the volume of 10% sodium bicarbonate solution. After the solution was filtered, an equal volume of petroleum ether was added to stir well. The hydrochloric acid solution was adjusted to pH5. After 10 hours of crystallization, the crystals were recrystallized with anhydrous methanol and dried to obtain 4.9 g of artemisinic acid with a content of 97.1%.

Embodiment 3

[0020] Take 5kg of dried Artemisia annua raw material, pulverize it to 80 meshes, place it in a CO2 supercritical extraction tank for extraction, introduce liquid CO2, the flow rate is 1ml / min / g raw material, the extraction temperature is 50°C, the pressure is 22Mpa, and the extraction time is 3h. The extract was dissolved with 35 times the volume of 8% sodium bicarbonate solution. After the dissolved solution was filtered, an equal volume of petroleum ether was added and stirred thoroughly. The hydrochloric acid solution was adjusted to pH4. After 25 hours of crystallization, the crystals were recrystallized with anhydrous methanol and dried to obtain 3.4 g of artemisinic acid with a content of 97.5%.

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Abstract

The invention discloses a method for preparing artemisinic acid, which is convenient to operate and has little pollution. The method is as follows: 1) crushing dry artemisia annua to 20-80 mesh crumbs, and placing the crumbs in a CO2 supercritical extracting tank which is filled with liquid CO2 for extraction, wherein the flow rate is 1-5ml/min/g raw material, the extraction temperature is 30-50DEG C, the pressure is 15-30Mpa, and the extraction time is 1-3h, and the extract is obtained after dissolving out; 2) dissolving the extract with a sodium bicarbonate solution, adding petroleum ether to the solution after filtration, adjusting to ph3-5 with hydrochloric acid solution, collecting a petroleum ether extraction liquid, decolorizing the extraction liquid with activated carbon, concentrating the extraction liquid to a small volume and crystallizing, and re-crystalizing crystals with anhydrous methanol to obtain the artemisinic acid. The artemisinic acid sample produced with the method has the advantages of high purity level, the industrialized production can be realized easily.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of artemisinic acid. Background technique [0002] Artemisinic acid is sesquiterpene acid, molecular formula C15H22O2, molecular weight 234.3, molecular structural formula: [0003] [0004] Artemisia annua is a plant in the Compositae family, and it is commonly used in folks in my country to treat malaria. Studies have found that Artemisia annua contains a large amount of sesquiterpene components, such as artemisinin, artemisinin, artemisinic acid, etc., which have insecticidal effects. [0005] Through literature search, the preparation method of artemisinic acid mostly uses organic reagents to extract silica gel column separation, such as "Studies on the structure of artemisinic acid". Such methods are complex in operation, low in extraction efficiency and serious in pollution, and are not suitable for industrial production. Contents of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C57/26C07C51/42
CPCY02P20/54
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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