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Method for facilitating microbe synthesis of 2,3-butanediol by adding exogenous factor

A technology for microbial synthesis and butanediol, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low substrate conversion rate, improve metabolic process, increase substrate conversion rate, and operate simple effect

Inactive Publication Date: 2007-12-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims at the problem of low substrate conversion rate in the process of bacterial biosynthesis of 2,3-butanediol, and provides an improved method for biosynthesizing 2,3-butanediol, that is, in the initial fermentation medium or in the fermentation process Add appropriate amount of exogenous additive factors to the fermentation broth to increase the conversion rate of the substrate and the fermentation level of 2,3-butanediol, and reduce the cost of production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add citric acid in the basic fermentation medium and make its initial concentration 0.05g / L, obtain the improved fermentation medium, take the basic fermentation medium as contrast, after fermentation 48h, utilize HPLC to detect and obtain the basic fermentation medium and the improved fermentation medium The residual glucose concentration in the fermentation medium was 0g / L, and the product 2,3-butanediol concentrations were 25.23g / L and 26.16g / L respectively. After adding 0.05g / L citric acid, the final concentration of the target product 2,3-butanediol increased by 3.56% compared with the control group.

[0036] The following 4 groups of experiments are to only change the concentration of citric acid in the fermentation medium, and other conditions remain unchanged. After the end of the experiment, 5 groups of data as shown in Table 1 are obtained, wherein the control refers to under the same conditions, without adding citric acid The fermentation results of the basal...

Embodiment 2

[0040] What is taken is not to add citric acid in the initial basal fermentation medium, but to select that the growth of the thalli reaches the exponential phase during the fermentation process (after about 12 hours of cultivation, the OD 600 =0.6), add citric acid in the fermented liquid, other conditions are with embodiment 1, and the results are as shown in table 2.

[0041] Table 2

[0042] Amount added

[0043] As can be seen from Table 2, citric acid is added to the fermentation broth when the bacterial growth reaches the exponential phase in the fermentation process. When the concentration of citric acid is 0.05~0.75g / L, the target product 2,3-butanediol The final concentration of can be increased by 7.62% to 22.13%.

Embodiment 3

[0045] Add α-ketoglutaric acid to the basic fermentation medium and make the initial concentration 0.05g / L to obtain an improved fermentation medium. Using the basic fermentation medium as a control, after 48 hours of fermentation, use HPLC to detect the basic fermentation medium The concentration of residual glucose in both base and improved fermentation medium was 0g / L, and the concentration of product 2,3-butanediol was 25.23g / L and 25.85g / L respectively. After adding 0.05g / L α-ketoglutarate, the final concentration of the target product 2,3-butanediol was increased by 2.40% compared with the control group.

[0046] The following 4 groups of experiments only changed the concentration of α-ketoglutaric acid in the fermentation medium, and other conditions remained unchanged. After the experiment, 5 groups of data as shown in Table 3 were obtained, wherein the control refers to under the same conditions. Fermentation results of basal fermentation medium without supplementatio...

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Abstract

The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for promoting the synthesis of 2,3-butanediol by microorganisms by adding exogenous factors. Background technique [0002] 2,3-Butanediol is widely used in many fields such as chemical industry, food, fuel and aerospace (Syu M J. Appl Microbiol Biotechnol, 2001, 55(1): 10-18). Its dehydration product, methyl ethyl ketone, is a solvent with a low boiling point, which can be used in coatings, adhesives, lubricants, dyes, inks and other industries, and can also be used as an intermediate for organic synthesis of fragrances and antioxidants; after esterification The dehydration product 1,3-butadiene is an important petrochemical basic organic raw material and synthetic rubber monomer; the octane isomer formed after concentrated dehydrogenation with methyl ethyl ketone can be used to produce high-grade aviation oil; its high Value derivatives 3-hydroxybutanone (ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 黄和纪晓俊李霜练敏杜军朱建国高振胡南
Owner NANJING UNIV OF TECH
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