Preparation method of freeze-dried powder containing stem-cell active factors

Abstract

The invention relates to the field of technical research of stem cells and provides a preparation method of freeze-dried powder containing stem-cell active factors excreted by human mesenchymal stem cells. Provided human adipose-derived stem cell raw materials are sufficient in source, safe and convenient to extract and safe and reliable in use and do not produce any untoward effect. The preparation method specifically comprises the following steps of exogenous factor detection, primary isolation, culture and cell passage of adipose-derived stem cells, identification of mesenchymal stem cells,collection of supernate, preparation of the freeze-dried powder, detection on the supernate and active factors of the stem cells of the freeze-dried powder and solvent preparation and filling. The prepared freeze-dried powder is easy to store and transport compared with the supernate, the activity of the factors is effectively saved, the freeze-dried powder can be applied to the beauty industry,the cell factor content is high, the moisture content in the freeze-dried powder is low, and accordingly the freeze-dried powder is easy to store. The freeze-dried powder prepared by adopting the preparation method is adopted after scalding, and the heal rate is high when the compound prepared from biological polysaccharide gel, collagen, folic acid and sterile ultrapure water is applied to wounds.

Description

technical field [0001] The invention belongs to the field of biotechnology medicine, relates to the field of stem cell technology research, in particular to a preparation method and application of a stem cell active factor secreted by human adipose mesenchymal stem cells. Background technique [0002] In recent years, with the deepening of research on mesenchymal stem cells, its secreted factors have become a hot spot for researchers. Mesenchymal stem cells can secrete a variety of biologically active cytokines, which can effectively regulate body cell signal transduction, activate human stem cells, and then physiologically repair or replace damaged, diseased and aging cells in the body. [0003] The application of stem cells in cosmetology is the earliest use of stem cells in plastic surgery. It is a stem cell cosmetology technology that has been studied more at present. The types of stem cells that are widely used in plastic surgery are mainly human umbilical cord mesench...

AI Technical Summary

Problems solved by technology

However, when the medium is used for conventional culture, the activity of stem cells is low, the content of secreted factors is very small, the yield is low, and the storage time is short. Using some conventional methods, such as mechanical friction, ultraviolet radiation and starvation culture, can not significantly improve Factor content in stem cell supernatant

At present, there are also some patent disclosures on the preparation methods of stem cell active factors and freeze-dried powders. For example, Chinese patent application 201710015650.7 discloses a human adipose-derived mesenchymal stem cell factor and its preparation method and use. The concentrated The content of mesenchymal stem cell factors in liquid adipose tissue has been improved to a certain extent, and the quality is stable. The product prepared from the composition with other materials in a specific ratio has a certain ability to repair skin damage; Chinese patent application 201610680823.2 discloses a human The preparation method of the freeze-dried powder of the culture supernatant of mesenchymal stem cells and the prepared freeze-dried powder, which preserve the various biologically active cytokine mixtures in the culture supernatant of human mesenchymal stem cells to the greatest extent, are used in the The application of cosmetics and other aspects has laid the foundation; but when the stem cell active factor or its freeze-dried powder prepared by the method disclosed in the above patent is applied to cell repair, not only the repair effect is not good, but the wound healing rate after burns is low. , during the preparation process, there is also a large loss of cytokines, and the moisture content in the freeze-dried powder is high, which leads to the problem that the freeze-dried powder is not easy to store

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