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Schizochytrium limacinum strain as well as mutagenesis method and application thereof

A technology of Schizochytrium and Schizochytrium, which is applied in the field of Schizochytrium strains and their mutagenesis, and can solve problems such as increased cost, low production efficiency, and complicated process

Inactive Publication Date: 2015-03-25
郭星
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies are all aimed at increasing the DHA content of fatty acids in bacterial strains, they often consider a single factor and have not comprehensively explored how to greatly increase the DHA content in bacterial strains, making the production of DHA complex, costly, and low in production efficiency. Industrialization of DHA Production by Schizochytrium

Method used

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  • Schizochytrium limacinum strain as well as mutagenesis method and application thereof
  • Schizochytrium limacinum strain as well as mutagenesis method and application thereof
  • Schizochytrium limacinum strain as well as mutagenesis method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The mutagenesis of Schizochytrium TC5-2 comprises the following steps:

[0052] S11. Cultivation of Schizochytrium: Using Schizochytrium before mutagenesis (schizochytrium strain isolated and screened from mangroves in Beihai Beach, Guangxi) as the starting strain, select a single colony of the starting strain, and ferment in 10mL liquid aseptically Medium (sterile fermentation medium contains the following ingredients: artificial seawater 600g / L; soybean peptone 30g / L; yeast extract 40g / L; glucose 90g / L; corn steep liquor 15g / L; multivitamin solution 5g / L; Grassone 20μmol / L. Among them, the formula of artificial seawater is: NaCl28g / L, MgCl 2 5g / L, KCl1g / L, CaCl 2 1.5g / L, NaHSO 4 6g / L, Na 2 CO 3 8g / L, Na 2 SO 4 10g / L, MgSO 4 3g / L, MnSO 4 5g / L, KH 2 PO 4 4g / L, K 2 HPO 4 7g / L, ZnSO 4 3g / L; the formula of multivitamin solution is: vitamin B 1 3g / L, vitamin B 2 10g / L, vitamin B 5 8g / L, vitamin B 6 4g / L, vitamin PP10g / L, biotin 12g / L, folic acid 45g / L, inos...

Embodiment 2

[0055] The mutagenesis of Schizochytrium TC5-2, the mutagenesis refers to the mutagenesis step of Example 1, wherein, in the ion beam mutagenesis step of S12, the dual plasma source pulse injection is made from the logarithmic growth phase bacterial liquid In the bacterium film that forms, injection dosage is the dosage of embodiment 2 in the following table 1.

Embodiment 3

[0057] The mutagenesis of Schizochytrium TC5-2, the mutagenesis refers to the mutagenesis step of Example 1, wherein, in the ion beam mutagenesis step of S12, the dual plasma source pulse injection is made from the logarithmic growth phase bacterial liquid In the bacterium film that forms, injection dosage is the dosage of embodiment 3 in the following table 1.

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Abstract

The invention provides a schizochytrium limacinum strain as well as a mutagenesis method and an application thereof. In the prior art, although how to improve the content of DHA in a strain is reported, since the consideration factor is often single, how to significantly improve the content of DHA in the strain is not comprehensively explored which also is a key to achieve the industrialization of schizochytrium limacinum. Schizochytrium limacinum TC5-2 is obtained by virtue of an ion beam mutagenesis method, by combining a culture medium, optimizing a fermentation process and adding an exogenous factor clethodim, the content of DHA in the strain is significantly improved which is conductive to achieving the industrialization of production of DHA by virtue of schizochytrium limacinum.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a Schizochytrium strain and a mutagenesis method and application thereof. Background technique [0002] Docosahexaenoic acid (DHA) is one of the polyunsaturated fatty acids necessary for the growth of mammals, but it is called an essential fatty acid because it needs to be taken from the outside. Studies have shown that DHA has very important physiological effects on infant brain and vision development, and has physiological functions such as prevention and treatment of cardiovascular diseases, prevention and treatment of senile dementia, and inhibition of cancer, so it is widely used in food, medicine and feed industries. [0003] The traditional source of DHA is deep-sea fish oil, but its existence rate is low, resources are limited, and purification process is complicated. Marine microorganisms are considered to be the most promising commercial source of DHA, and the use o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N13/00C12P7/64C12R1/645
CPCC12N1/14C12N13/00C12P7/6427C12N1/145C12R2001/645
Inventor 郭星梁世中
Owner 郭星
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