The invention discloses a whole blood autoluminescence immunoassay point-of-care test instrument. The instrument sequentially comprises an integrated reagent tube storing and fetching device, an automatic sampling device, an item group parallel reaction treatment device, a washing and substrate adding device and a photon detection counter. By the aid of the item group parallel reaction treatment device, test items of any patient can be parallelly operated, detected and reported quickly by means of item integrated cards, so that test efficiency is improved. By intelligent and automatic sample adding, indexes of each item group can be tested parallelly, so that sample adding time is saved. By onsite detection for filter pressing and washing, operations required by each integrated reagent of each item group are handled automatically, and accordingly test efficiency is improved. By pulsed injection of washing liquor for washing and continuous pump drainage of waste liquid, quickness in washing is achieved, and integral operating speed is increased. By matching of the instrument and the integrated reagents, blood cells can be well separated, point-of-care test of a whole blood sample can be realized, and suitableness for samples of whole blood, serum, plasma, other body fluids and the like is realized.