Method for detecting HSL gene expression in bovine muscle
A technology for gene expression and bovine muscle, applied in the field of genetic engineering, to achieve the effect of simple method and easy operation
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[0020] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.
[0021] A method for detecting bovine muscle HSL gene expression, the specific steps are as follows:
[0022] 1) Design the specific primer pair H-F and H-R of the HSL gene sequence and the primer pair G-F and G-R of the internal reference gene:
[0023] Referring to the HSL gene sequence (Genbank Accession No.NM-001080220) recorded in Genbank, and the internal reference gene GAPDH (Genbank Accession No.0NM-001034034), primers were designed using Primer Premier 3.0 software, and the specificity of the primers was tested through the Blast function in NCBI .
[0024] H-F: 5'-GATGAGAGGGTAATTGCCG-3', see SEQ NO.1 in the sequence listing.
[0025] H-R: 5'-GGATGGCAGGTGTGAACT-3', see SEQ NO.2 in the sequence listing.
[0026] The sequence of the primer p...
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