Porcine skeletal muscle satellite cell culture medium PM+ and application thereof

A satellite cell and cell culture technology, applied in the direction of cell culture active agent, bone/connective tissue cells, culture process, etc., can solve the problem of slow cell proliferation, difficulty in maintaining the characteristics of skeletal muscle stem cells, and unsatisfactory PSC culture medium, etc. question

Active Publication Date: 2016-12-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no unified PSC medium, and most of the PSC medium culture conditions are not ideal, the cell proliferation rate is slow, and

Method used

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  • Porcine skeletal muscle satellite cell culture medium PM+ and application thereof
  • Porcine skeletal muscle satellite cell culture medium PM+ and application thereof
  • Porcine skeletal muscle satellite cell culture medium PM+ and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Medium PM+ composition and its preparation:

[0026] 1. Preparation of gentamicin, basic fibroblast growth factor and chicken embryo extract storage solution:

[0027] (1) Preparation of gentamicin solution: Accurately weigh 1g of gentamicin powder and dissolve it in 20mL of nuclease-free water to prepare a 50mg / mL gentamicin solution, store in -20°C for later use ;

[0028] (2) Preparation of basic fibroblast growth factor solution: Accurately weigh 10 μg of basic fibroblast growth factor powder and dissolve it in 100 μL of nuclease-free water to prepare a 100 ng / μL bFGF solution, which is the basic fibroblast growth factor storage solution; after aliquoting, store at -20°C for later use;

[0029] (3) Preparation of the chicken embryo extract solution: add 10 mL of nuclease-free water to the chicken embryo extract powder, fully dissolve to obtain a storage solution, and store it at -20°C for later use;

[0030] 2. Antibiotics-antimycotics and fetal bovine serum are ...

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Abstract

The invention belongs to the technical field of animal cell culture, and particularly relates to a porcine skeletal muscle satellite cell culture medium PM+ and application thereof. Every 1L of the orcine skeletal muscle satellite cell culture medium PM+ comprises 20% of fetal calf serum (FBS), 1% of cell culture additive (GlutaMax), 1% of non-essential amino acid (NEAA), 1% of antibiotic-antifungal (AA), 0.5% of chick embryo cultyre, 50 mg/L gentamycin, 25 mu L/L alkaline fibroblast growth factor, and the balance of RPMI 1640 liquid basal culture medium. The invention also discloses application of the culture medium.

Description

technical field [0001] The invention belongs to the technical field of animal cell culture, and in particular relates to a medium PM+ for porcine skeletal muscle satellite cells and an application thereof. Background technique [0002] Pork is one of the main meats consumed in my country. The growth and development of pig skeletal muscle is directly related to the economic benefits of pork. The research results show that the growth and development of porcine skeletal muscle is closely related to the function of its satellite cells (porcine satellite cells, referred to as PSC). PSCs belong to skeletal muscle stem cells, and usually exist between the basement membrane and the muscle cell membrane in a resting state. After the skeletal muscle is stimulated, it will activate, proliferate, differentiate from the resting state, and fuse to form myotubes, causing muscle fiber hypertrophy, which in turn causes muscle fiber type conversion [1] . In recent years, there have been ma...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0658C12N2500/32C12N2500/80C12N2501/115
Inventor 曹建华王昇任瑞敏黄英赵书红
Owner HUAZHONG AGRI UNIV
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