28 results about "Gentamycins" patented technology

The invention relates to application of golden larch bark acetic acid in preparation of methicillin-resistant staphylococcus aureus (MRSA) biological membrane and bacterium holding inhibitor. A biological membrane formation inhibition experiment shows that the pseudolaric acid can remarkably inhibit formation of an MRSA biological membrane, a fluorescent quantitative PCR experiment shows that the pseudolaric acid remarkably reduces transcription of related genes of the MRSA biological membrane, and a persistent bacterium killing experiment shows that the pseudolaric acid enhances the killing capability of gentamicin on MRSA persistent bacteria. Lung epithelial cell adhesion tests find that the golden larch bark acetic acid significantly reduces the adhesion effect of the MRSA strain on epithelial cells. A test for constructing an MRSA infected greater wax moth model shows that the golden larch bark acetic acid has a system protection effect on the MRSA infected greater wax moth model.

The invention discloses an amplification culture solution of TILs cells and a culture method thereof. The amplification culture solution comprises a complete culture medium, and an anti-BTLA antibody with the final concentration of 0.1-10 [mu] g / mL, penicillin with the final concentration of 50-500 [mu] g / mL, streptomycin with the final concentration of 50-500 [mu] g / mL, gentamicin with the final concentration of 10-100 [mu] g / mL and amphotericin with the final concentration of 1-50 [mu] g / mL are added into the complete culture medium. The anti-BTLA antibody component in the culture solution can relieve inhibition of BTLA on T cells by blocking combination of BTLA and a ligand HVEM of BTLA, so that the anti-tumor capability of TILs is enhanced; meanwhile, after the BTLA is combined with the ligand HVEM, T cell activation and proliferation can be inhibited, and rapid proliferation of TILs is promoted; the irradiation PBMC added in the TILs cell culture process can provide an activation signal for the TILs and drive rapid proliferation of the TILs cells.

The present disclosure relates to a method of treatment or prophylaxis of a preterm infant by administering intravenously a therapeutic amount of a composition comprising IGF-1 and IBGBP-3 in combination with a therapy selected from the group comprising: caffeine, fentanyl, fluconazole, gentamicin, insulin, midazolam, morphine, low dose norepinephrine, vancomycin concentrations of 5 mg / ml or less, parenteral nutrition (or a composition for the same use) and combinations thereof.

The application discloses a material for preventing tissue adhesion and hemostasis and a preparation method thereof, and relates to the technical field of medical biomaterials.The carboxymethyl cellulose, chitosan and vancomycin / ceftazidime are synergistically matched, so that the three functions of hemostasis, adhesion prevention and antibiosis are integrated;meanwhile, the water absorption and gelation of the carboxymethyl cellulose and the blood coagulation activation of the chitosan can jointly strengthen the hemostasis effect; the tissue barrier function of the chitosan and the gel isolation of the carboxymethyl cellulose can jointly improve the adhesion prevention performance; the broad-spectrum antibiosis of the vancomycin and the ceftazidime can respond to the infection risk in different surgical scenes; the material preparation process is simple, the dosage form can be adjusted according to different surgical scenes, the material has good biocompatibility and can be completely degraded, the problem that the existing medical materials have single function and are difficult to meet the needs of complex surgical wounds is solved, and the material has significant clinical application value.

The invention belongs to the field of microbial engineering, and provides a gentamicin C1a high-yield biological manufacturing method based on metabolic network modeling and reverse metabolic engineering renovation, which comprises the following steps: S1, constructing a genome scale metabolic model of a target production strain, predicting and screening a first target gene in positive correlation with gentamicin biosynthesis by using the model; s2, performing whole genome sequencing on the gentamicin high-yield mutant strain, and identifying a second target gene related to the high-yield character through comparative genomics analysis; s3, combining the first target gene predicted in the step S1 and the second target gene identified in the step S2, and constructing to obtain a recombinant engineering strain; s4, carrying out fermentation culture on the recombinant engineering strain, and supplementing materials in the fermentation process; by remarkably improving the production efficiency and strain performance of gentamicin C1a, important technical support and theoretical basis are provided for green biological manufacturing of antibiotics.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

The invention discloses application of gentamicin and ceftazidime in enhancing the drug sensitivity of serratia marcescens, and belongs to the field of biological medicine. The serratia marcescens is a strain carrying drug-resistant genes such as OqxB and mdtC, the mass ratio of gentamicin to ceftazidime is (1: 10)-(1: 50), the synergistic effect of gentamicin and ceftazidime is the strongest under the ratio, the sensitivities of serratia marcescens to gentamicin and ceftazidime can be recovered by 12.5 times and 42 times respectively, MBC is synchronously and greatly reduced, and the serratia marcescens has the dual effects of drug sensitivity enhancement and infection treatment; the single dosage is reduced, so that the cost is reduced, and the potential damage of the medicine to a host is reduced while the treatment effect is ensured. The prodigiosin is effective to serratia marcescens carrying various drug-resistant genes such as OqxB, mdtC, AAC (6 ')-Ic, KdpE and CpxA, cost is saved for prevention, control and treatment of serratia marcescens in animal husbandry, and important reference is provided for safe extraction and utilization of prodigiosin and clinical treatment of human serratia marcescens infection.

The utility model relates to injection filtering technical field, concretely is a kind of gentamicin sulfate injection filtering device, including screening box and the liquid inlet cylinder of top intercommunication thereof, the baffle is arranged in screening box and is divided into left and right two groups of screening cavities, filter cloth is arranged in each group of screening cavities, the bottom intercommunication of screening box has liquid outlet pipe, and first solenoid valve is arranged on liquid outlet pipe to control the opening and closing of liquid outlet pipe.The utility model is controlled by double screening cavities and solenoid valve interconnection design, realizes the automatic switching of filtering path, ensures production continuity and liquid processing efficiency;Combined with high-pressure flushing from bottom to top and overflow discharge mechanism, can efficiently remove blocked impurities without disassembling filter cloth, reduce downtime maintenance time, prolong filter cloth life.

The multi-dimensional plasmonic coupling system based on Ag@PVP and Au@Ag NCs for surface-enhanced Raman spectroscopy (SERS) detection of gentamicin relates to the field of analytical detection, and adopts hydrothermal synthesis to prepare Ag@PVP core-shell nanoparticles and Au@Ag core-shell cubes (Au@Ag NCs). Ag@PVP is induced to agglomerate by gentamicin, and then Au@Ag NCs are introduced to change the electromagnetic field of the nanoparticle agglomeration, so as to change the Raman characteristic peaks of gentamicin and distinguish it from its homologues. Under the optimized experimental conditions, the linear detection range of gentamicin is 10 ‑5 ~10 ‑9 M, the detection limit is 6.23 × 10 ‑10 M, and the relative standard deviation of SERS intensity is less than 10%. The method has the advantages of simple operation, high accuracy and sensitivity, and does not need complex operation technology, and meets the requirements of large quantities and rapid analysis and detection.

This invention discloses a three-dimensional multilayer sheet-like nanozyme, its preparation method, and its application in the immunochromatographic detection of small molecule environmental pollutants, belonging to the field of nanozyme and immunochromatographic detection technology. The three-dimensional sheet-like nanozyme is meticulously designed by continuously constructing Au nanoparticles (NPs) and AuPt bimetallic NPs on flexible graphene oxide (GO) nanosheets. Beneficial effects: In the nanozyme, two-dimensional GO provides a large surface area and high stability, hundreds of large AuNPs provide enhanced colorimetric ability and a large spherical surface area, and tens of thousands of AuPt satellites act as spatial catalytic sites and produce excellent peroxidase-like activity. The proposed bimodal ICA can simultaneously detect three important drugs, namely gentamicin, clenbuterol, and ractopamine, in real complex samples, greatly improving the detection performance of existing colorimetric ICA techniques based on simple catalysis, thus showing great application potential in real-time monitoring of small molecule targets under different conditions.

The invention discloses a method for detecting influence of bacteria on tumor cell proliferation based on CCK8, which is characterized by comprising the following steps: adding a trigeminy antibiotic culture medium containing penicillin-streptomycin-gentamicin into a bacteria and cell co-culture system to treat the co-culture system; comprising the following steps: centrifuging a cell suspension, discarding a supernatant, adding an antibiotic-free DMEM culture medium, uniformly mixing, and adding cells into a 96-well plate for overnight culture; centrifuging fresh bacterial liquid, discarding supernatant, adding a DMEM (Dulbecco Modified Eagle Medium) without antibiotics, uniformly mixing, adding bacteria into a 96-well plate, culturing, discarding an original culture medium, adding a DMEM containing penicillin-streptomycin-gentamicin antibiotics, and culturing; the method comprises the following steps of: sucking and abandoning a culture medium in a 96-well plate, adding a DMEM (Dulbecco Modified Eagle Medium) containing 10vt% of CCK8 reagent, measuring an absorbance value by using a microplate reader, and judging the influence of bacteria on tumor cell proliferation. The method has the advantages of higher stability and repeatability, and more reliable experimental result.

This invention relates to the field of immunology, and more particularly to a hybridoma cell line and its secreted monoclonal antibody against the metamizole residue marker 4-methylaminoantipyrine (MAA), and its applications. This invention uses 4-aminoantipyrine to react with methyl 4-bromomethylbenzoate, followed by alkaline hydrolysis to obtain a hapten (Formula I). ​​An immunogen is then synthesized and used to immunize mice. After cell fusion and screening, a hybridoma cell line capable of secreting a monoclonal antibody recognizing MAA is prepared, named Metamizole-6A4. This hybridoma cell line has a high efficiency in secreting monoclonal antibodies. The secreted monoclonal antibody can accurately detect MAA, IC50, and other markers. 50 The concentration is 4.06 ng / mL, with high specificity. Cross-reactivity with aminopyrine and its metabolites 4-aminoantipyrine, 4-formamidoantipyrine, 4-acetamidoantipyrine, sulfadiazine, norfloxacin, lincomycin, tylosin, penicillin G, streptomycin, and gentamicin is less than 5%. It can accurately detect MAA residues in food. Formula I.

The application belongs to the technical field of microorganisms, and discloses a method for inducing formation of living unculturable state bacteria in the presence of host cells and application thereof. The method is as follows: cells in the 3th to 20th generation are transferred to a well plate to obtain induced cells; bacteria in the logarithmic growth phase are washed by a buffer, and modified cell culture solution is resuspended to obtain a bacterial suspension. The bacterial suspension is added to the induced cells, and after incubation in a constant-temperature incubator for 1-5 hours, 10-500 mg / L gentamicin-containing modified cell culture solution is added. After cell treatment for 3-10 days, intracellular bacteria are collected by adding cell lysis solution, and then washed, centrifuged and resuspended to obtain living unculturable state bacteria. The application can induce bacteria to enter the living unculturable state in the presence of host cells, which not only expands the understanding of living unculturable state bacteria, but also provides theoretical and technical support for recurrent and chronic infectious diseases in clinical practice.

The present invention relates to a pharmaceutical composition for preventing or treating hearing loss or tinnitus, comprising visomitin as an active ingredient. In the present invention, visomitin significantly inhibited ROS production induced by gentamicin or cisplatin treatment. In particular, visomitin exhibited an effect of inhibiting the cytotoxicity of gentamicin in an HEI-OC1 cell line and in cochleas extracted from mice in vitro experiments. In addition, in an in vivo experiment using zebrafish, visomitin significantly inhibited ROS generation in hair cells and apoptosis of hair cells caused by gentamicin treatment. The results demonstrate a superior improvement effect compared to antioxidants used as control drugs, and it is considered that visomitin can be used for preventing or treating hearing loss caused by toxicity of antibiotics or anticancer drugs.

The invention discloses a multi-immunochromatography test strip with unified detection limit for detecting four aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a colloidal gold pad, a sample pad and a PVC (polyvinyl chloride) bottom plate, wherein a gold-labeled antibody is fixed on the surface of the colloidal gold pad, and when a sample flows through the colloidal gold pad, the gold-labeled antibody is dissolved and serves as a tracer molecule to migrate along with a liquid phase; four detection areas T1, T2, T3 and T4 and a quality control line area C are arranged on the detection pad, a kanamycin antigen, a gentamicin antigen, a neomycin antigen and a spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse antibody is sprayed on the quality control line area C. According to the present invention, the expensive reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting kanamycin, gentamicin, neomycin and spectinomycin, and the detection limits can achieve 5 ng / mL.

The invention relates to the technical field of fermentation engineering, and particularly discloses an optimization method for improving gentamicin C1a production based on artificial neural network and genetic algorithm coupled fermentation kinetics, comprising the following steps: S1, metabolic modeling and key factor identification; s2, performing multi-objective optimization; s3, intelligent monitoring and feedback control; the invention develops an intelligent control system which is used for optimizing the fermentation process of gentamicin C1a and comprises four core modules, namely a metabolic modeling module, a key factor identification module, a multi-objective optimization module and an intelligent monitoring module. Based on a BPNN model, fermentation dynamics is modeled, a nonlinear relation between different specific rates is disclosed, and an optimal control parameter range of different stages of a fermentation process is determined by using a BPNN-NSGA-II multi-objective optimization strategy; the total sugar on-line monitoring system based on the near infrared spectrum and the Raman spectrum is constructed, and multi-source spectrum data and a machine learning algorithm are fused, so that the prediction precision of the total sugar is improved.

The invention discloses a semen diluent for improving the production efficiency of pig semen, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises an antioxidant and a composite antibacterial agent, the nutritional agent is composed of glucose and fructose, the antioxidant is glutathione, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. Accordingly, a corresponding preparation method and a using method are also established. Researches show that the diluted boar semen can survive at 17 DEG C for more than or equal to 144 hours, the activity is more than or equal to 60%, 1 part of the original semen with the sperm density of 200 mL and the sperm density of 1 billion / mL can cover 40 sows, the utilization rate of the original semen is increased by 150%, the breeding cost is remarkably reduced, and the method is suitable for large-scale popularization.

The invention relates to the field of veterinary drugs, in particular to a pharmaceutical composition for preventing and treating rabbit dermatomycosis as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw materials in percentage by weight: 0.5%-2% of clotrimazole, 1%-5% of 5% gentamicin sulfate powder, 20%-40% of sulfur powder, 50%-75% of talcum powder, 2%-6% of 32.5% neomycin sulfate powder and the balance of water. According to the composition, clotrimazole is introduced as a core antifungal component, a targeting action mechanism of specific inhibition of fungal cytochrome P-450 of clotrimazole is utilized, and a non-specific bactericidal effect of sulfur powder is combined, so that synergistic interaction is realized.

The present invention provides an antimicrobial combination comprising three different antimicrobial agents The first antimicrobial agent is selected from ceftazidime, polymyxin E, polymyxin B, and pharmaceutically acceptable derivatives thereof; the second antimicrobial agent is selected from zidovudine, doxycycline, fosfomycin and pharmaceutically acceptable derivatives thereof; and the third antimicrobial agent is selected from levofloxacin, doxycycline, fosfomycin, meropenem, rifampicin, gentamicin, polymyxin B / E, and pharmaceutically acceptable derivatives thereof; wherein the combination includes at least one of levofloxacin, doxycycline, rifampicin, fosfomycin, or a pharmaceutically acceptable derivative thereof; provided the combination is not (1) polymyxin E / B, zidovudine and rifampicin or (2) ceftazidime, zidovudine and fosfomycin. Also provided is an antimicrobial combination comprising three antimicrobial agents, wherein the first antimicrobial agent is ceftazidime or a pharmaceutically acceptable derivative thereof; the second antimicrobial agent is zidovudine or a pharmaceutically acceptable derivative thereof; and the third antimicrobial agent is polymyxin E or a pharmaceutically acceptable derivative thereof.

The invention relates to the field of pig semen preservation, in particular to a 15-20 DEG C diluent of pig semen with ferulic acid as an additive as well as a preparation method and application of the 15-20 DEG C diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.715 g of glucose, 0.125 g of sodium citrate, 0.125 g of sodium ethylene diamine tetracetate, 0.075 g of potassium chloride and 0.03 g of gentamicin sulfate into 100 mL of double distilled water, and after the materials are fully dissolved, the pH value of the solution is adjusted to 6.80-7.00, and the osmotic pressure of the solution is adjusted to 290-300 mOsm / kg. The method has the advantages that ferulic acid is used as a protective agent for preservation of the boar semen at 15-20 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the vitality, motility, acrosome integrity and plasma membrane integrity of boar sperms preserved at 17 DEG C can be remarkably improved, and the fertilization vitality of the sperms is kept.

The invention relates to a purification method of an isepamicin sulfate crude product. Impurities comprise an impurity A, 3N-isepamicin, gentamicin B and the like. The method comprises the following steps: adding an isepamicin sulfate crude product aqueous solution into a water suspension generated by dialdehyde cellulose and sodium hydrogen sulfite, mixing and stirring while adjusting the pH value to 4-5 by using dilute sulphuric acid, generating Schiff base through a chemical reaction between amino groups of impurities and dialdehyde cellulose, forming impurity-loaded cellulose, and filtering to remove impurities, thereby obtaining the isepamicin sulfate crude product. The purity is greater than or equal to 99.2%, the yield is greater than or equal to 94.4%, and the dialdehyde cellulose can be regenerated and recycled. The method is simple to operate, high in yield and low in cost.

The invention belongs to the technical field of microorganisms, and particularly relates to a Bifidobacterium longum subsp. Longum FMBL B250365 MQ which is preserved in the China Center for Type Culture Collection on September 18, 2025, has the preservation number of CCTCC M 20252060, can utilize oligosaccharides such as fructo-oligosaccharide, galactooligosaccharide, maltooligosaccharide, soybean oligosaccharide and the like, and can be used for preparing a feed additive, a feed additive and a feed additive. Good tolerance to acid and cholate is achieved; the compound is sensitive to ampicillin, streptomycin, tetracycline, chloramphenicol, vancomycin, erythromycin, clindamycin and gentamicin; the drug resistance to kanamycin is realized; the caenorhabditis elegans has antioxidant and bacteriostatic capabilities, and the average life of caenorhabditis elegans is prolonged by 18.65%; the compound can be used for preparing anti-aging and life-prolonging medicines, and has a wide application prospect.

The invention provides blood vessel promoting and antibacterial double-effect composite bone cement and a preparation method thereof, and belongs to the technical field of medical biological materials. The preparation method comprises the following steps: carrying out esterification reaction on methacrylic anhydride and hydroxyl of bacterial cellulose (BC) in anhydrous N, N-dimethylformamide to prepare modified cellulose (BC-MA) of which the surface contains methylacryloyl; the preparation method comprises the following steps: mixing BC-MA with an ethanol solution containing Roxadustat, and carrying out vacuum drying, so as to obtain a medicine carrying material BC-MA (at) Roxa; then spraying a high-concentration gentamicin sulfate aqueous solution on the BC-MA-coated Roxa, and carrying out freeze drying and grinding, so as to obtain a double-drug carrier BC-MA-coated Roxa-coated Genta; and mixing the powder with PMMA bone cement powder in proportion, adding a liquid-phase monomer, stirring and curing to obtain the BC-MA-coated Roxa-coated Genta / PMMA composite bone cement. The BC-MA (at) Roxa (at) Genta / PMMA composite bone cement prepared by the invention has relatively high porosity and connectivity, high drug loading capacity, stable release process and long duration time. The material realizes the functionalization of antibiosis and blood vessel promotion, and provides good biological activity and tissue integration performance for bone repair.

The present invention generally relates to the field of isotopically labeled and unlabeled gentamicin congeners and to methods for synthesizing such congeners. In particular, the present invention relates to at least one isotopically labeled gentamicin C or its salts, solvates, or derivatives. 13 C, D and / or 15 Gentamicin C containing an N atom, and gentamicin C or its salts, solvates, or derivatives, in particular, at least one 13 C, D and / or 15 This invention relates to a method for preparing gentamicin C containing a nitrogen atom, or its salts, solvates, or derivatives.

The invention discloses a preparation method of an antibody for detecting ovalbumin and vancomycin in a biological product, the ovalbumin antibody is obtained by directly immunizing an animal, the vancomycin antibody is prepared by carrying out nucleophilic substitution reaction on vancomycin and N-(P-maleimidophenyl) isocyanate to obtain a vancomycin hapten, and the vancomycin hapten is used for detecting the ovalbumin and vancomycin in the biological product. The vancomycin immunogen is obtained by coupling the vancomycin with carrier protein, and then animals are immunized to obtain the vancomycin immunogen. The antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting ovalbumin contained in a biological product, the detection limit is 200 g / L; when the kit is used for detecting vancomycin contained in a biological product, the detection limit is 0.5 g / L. The detection result is high in accuracy, especially in a vancomycin antibody specificity experiment, the detection result shows that the detection result has remarkable specificity on vancomycin, and the detection result is not interfered by drugs such as polymyxin E, kanamycin, streptomycin, gentamicin, penicillin, tetracycline, chloramphenicol, neomycin, aureomycin, oxytetracycline and doxycycline.