224 results about "Gentamycins" patented technology

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

The invention discloses a cryopreservation solution and cryopreservation resuscitation method for liver primary cells and relates to the field of biotechnologies. The cryopreservation solution is prepared according to the following formula, including 1,000g of dH2O, 15.6g of DMEM / F12 culture medium powder, 1.2g of sodium bicarbonate, 300ng of dexamethasone, 10 micrograms of liver primary cell growth factor, 25mg of insulin, 0.3g of glutamine, 25mg of gentamycin, 50mg of penicillin, 25mg of clarithromycin, 0.1g of DMSO, 10g of PEG6000, 1g of polyvinyl pyrrolidone and 1g of 1,3-propylene glycol. According to the cryopreservation solution and cryopreservation resuscitation method for the liver primary cells, the problem that the liver primary cells are relatively low in survival rate after cryopreservation resuscitation and cannot adhere to walls for drug metabolism study is solved, and the developed new cell cryopreservation solution formula and the corresponding resuscitation method can enable the cryopreservation resuscitation survival rate of the cells to be over 80%.

The invention provides a preparation method and application of a product obtained by coupling gentamicin with carrier protein, as well as a gentamicin antibody. Animals are immunized with gentamicin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting gentamicin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; the gentamicin coupled with the carrier protein is used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-gentamicin antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the gentamicin, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling gentamicin with carrier protein, as well as the gentamicin antibody can serve the rapid detection of gentamicin residue in foods.

The invention discloses a gentamycin immunoassay reagent as well as a preparation method and a detection method thereof. The gentamycin immunoassay reagent comprises enzyme labeled gentamycin and an indicator reagent used for detecting a gentamycin antibody-enzyme labeled gentamycin compound; and the enzyme labeled gentamycin formed by coupling the gentamycin to glucose dehydrogenase. The gentamycin immunoassay reagent provided by the invention can accurately and rapidly determine the content of the gentamycin in a sample such as human blood. Compared with the existing detection reagent on themarket, the gentamycin immunoassay reagent has the advantages of convenience, rapidness, high sensitivity, strong specificity, accuracy in quantification and the like and is beneficial to clinical popularization and use.

A method of treating prostate cancer in a living mammal includes local administration of a composition that includes a therapeutically effective concentration of collagenase. In one embodiment, a method of treating prostate cancer in a living mammal includes local administration of a composition that includes a therapeutically effective concentration of collagenase and at least one of a glycosidase, a protease, a nuclease, a lipase, an esterase, a plasminogen activator, a streptokinase, and combinations thereof. Preferably a glycosidase, such as, for example, hyaluronidase, is administered. Compositions used in methods for treating prostate cancer can also include or be administered with calcium ions, a nonionic surfactant, such as, for example, Triton(R) X-100, and an antibiotic, such as, for example, gentamicin. Another method of treating prostate cancer in a living mammal includes activating PSA in vivo by, for example, locally administering calcium ions.

The invention discloses streptomyces albulus (GS-114) and a method of the same to prepare epsilon-polylysine, and belongs to the field of microbial fermentation engineering. The provided streptomycesalbulus (GS-114) has resistance to streptomycin, gentamicin and rifamycin with 3 [mu]g / mL, 1 [mu]g / mL and 0.1 [mu]g / mL or higher concentrations, and can efficiently synthesize epsilon-PL in quantity,the yield with 192 h of fermentation can reach 56.3 g / L, and production efficiency can reach up to 7.03 g / L / d. The streptomyces albulus (GS-114) also has good passage stability and has strong potential for the industrial production of the epsilon-PL.

A medicinal film for treating oral ulcer is composed of a plastics coated paper layer, a composite film layer, and a medicine layer as sandwich which is proportionally prepared from gentamycin sulfate, menonasal and dexamethasone acetate. Its preparing process includes preparing film slurry, preparing medicine slurry defoaming and preparing film.

The invention relates to a method for detecting aminoglycosides in milk, which comprises the following steps: (1) extracting; (2) preparing a standard working solution; (3) performing determination bya high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS), wherein the aminoglycosides are paromomycin, tobramycin, gentamicin, kanamycin, amikacin, apramycin, streptomycin, dihydrostreptomycin, neomycin, spectinomycin and hygromycin B. An MIP solid phase extraction column is uses in the step of extracting, thereby simplifying the pretreatment step, reducing the consumption of reagents and the time uses for the pretreatment; a SiELC Obelisc R chromatographic column is used for the separation of 11 kinds of aminoglycosides; and the mobile phase does not require heptafluorobutyric acid and does not contaminate the mass spectrometry.

A method of using immune chromatography semi ¿C quantitative test paper to detect gentamicin sticks sample solution absorbing portion, colloidal gold labeled portion, detecting reaction portion and water absorbing portion in sequence on backing of test paper. The detecting reaction portion is prepared as coating 1 ¿C 3 bar of gentamicin antibody or detection antigen as detection line, coating 1 ¿C 3 bar of IgG for anti ¿C second generic animal protein as reference line and using 2 ¿C 4 bar of combination line formed by said two lines being used no more than one bar at the same time in combination.