13 results about "Piperacillin" patented technology

The invention relates to a primer and probe combination for nested PCR detection of urinary tract klebsiella pneumoniae and drug-resistant genes and a kit thereof, and primer and probe sequences designed in the invention do not cause cross amplification of other urinary tract pathogenic bacteria, and can meet specific requirements. In addition, exogenous antibiotic interferents such as gentamicin, tobramycin, ceftazidime, levofloxacin, piperacillin and nitrofurantoin have no influence on experimental results, and the anti-interference requirement is met. The prepared kit can be used for simultaneously detecting the urinary tract klebsiella pneumoniae and the drug-resistant gene, and the detection is convenient, simple and reliable. Compared with common strain identification, drug resistance gene detection can guide clinical medication more accurately, and invalid treatment is avoided.

The present invention relates to an antimicrobial composition of a penicillin, a β-lactamase inhibitor and an ion-chelating agent. More particularly, the present invention relates to an antibiotic composition of piperacillin, avibactam and EDTA in a predefined ratio for the effective management of bacterial infections by decreasing the MIC and increasing the stability of the composition.

The invention provides Kineococcus G2 and application of the Kineococcus G2 in preparation of a pigment with antioxidant activity, the Kineococcus new strain G2 is CCTCC No. M20242602, the strain is spherical, the size is 1.0 * 1.5 mu m, the optimal growth temperature is 30-37 DEG C, the pH value is 7.0-9.0, and the salt concentration is 1.0%-2.0%. The strain G2 is not sensitive to bacitracin, ciprofloxacin and norfloxacin, and is sensitive to novobiocin, polymyxin, gentamicin, piperacillin, ofloxacin, erythromycin, streptomycin, kanamycin, rifampicin, ampicillin, vancomycin, carbenicillin, chloramphenicol, tetracycline, penicillin, cefoperazone, oxacillin, amoxicillin and neomycin. Pigment generated by fermentation of the G2 strain has good antioxidant activity, and when the concentration is 10 micrograms / mL, the scavenging activity on DPPH is 25.00%, the scavenging activity on hydroxyl radicals is 80.58%, and the iron ion reducing capacity is 1.57, which are all higher than those of contrast beta-carotenoid. The application of the new Kineococcus strain G2 can lay a foundation for the research of antioxidant drugs such as medical treatment and health care product development.

The invention belongs to the technical field of microorganisms, and particularly relates to multi-drug-resistant klebsiella pneumoniae and application thereof in construction of a pneumonia animal model. Specifically, the multi-drug-resistant klebsiella pneumoniae BKP919 provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36301, and the multi-drug-resistant klebsiella pneumoniae BKP919 has wide drug resistance to various antibiotics such as amoxicillin, piperacillin and the like. The bacterial liquid of the strain is used for carrying out one-time intratracheal instillation on a mouse in a nasal instillation manner, and a stable severe pneumonia model can be established within 48-72 hours. The model preparation method is simple to operate and high in efficiency, and the model mouse has remarkable pneumonia symptoms, obvious pathological change and good related index stability, and can be used for pathogenesis research and targeted drug development of multi-drug-resistant klebsiella pneumonia infected pneumonia, so that the model has good practical application value.

This application relates to the field of drug synthesis technology and provides a method for preparing levofloxacin. The method includes first mixing N,N-dimethylaminoacrylate, triethylamine, and toluene, then adding 2,3,4,5-tetrafluorobenzoyl fluoride dropwise and reacting it with L-2-aminopropanol. After post-treatment, a toluene solution of the amine is obtained. Next, using N,N-dimethylformamide or toluene as a solvent, a cyclization reaction is carried out with differentiated feeding methods and temperature control. The product is purified by hot slurrying with a non-alcoholic organic solvent or a saturated alcohol solution of the levofloxacin cyclase. Finally, the product is purified by acidic hydrolysis, piperacillin reaction, and dimethyl sulfoxide to obtain high-purity levofloxacin. This application optimizes solvent selection and process integration, shortens reaction time, improves product yield and purity, reduces waste emissions, allows for resource utilization of byproducts, and features a simple and easily industrialized process suitable for large-scale production.

The present invention belongs to the detection technology of pharmaceutical preparations, and specifically relates to a detection method for related substance II in piperacillin sodium and tazobactam sodium for injection, wherein high performance liquid chromatography (HPLC) is used to detect related substance II in piperacillin sodium and tazobactam sodium for injection, and the method comprises the steps of (1) HPLC detection conditions (2) solution preparation (3) determination, etc. The detection method can simultaneously and effectively detect piperacillin impurity U and piperacillin impurity V, has good impurity separation effect, and can accurately calculate the content of any impurity by peak area according to the main component external standard method of the added factor through the detection result, which is of great significance for product quality control.

According to the primer and probe combination and the kit for nested PCR detection of the proteus mirabilis and the drug-resistant gene in the urinary tract, the designed primer and probe sequences do not cause cross amplification of other urinary tract pathogenic bacteria and can meet the specific requirement. In addition, exogenous antibiotic interferents such as gentamicin, tobramycin, ceftazidime, levofloxacin, piperacillin and nitrofurantoin have no influence on experimental results, and the anti-interference requirement is met. The prepared kit can be used for simultaneously detecting the proteus mirabilis and the drug-resistant gene of the urinary tract, and the detection is convenient, simple and reliable. Compared with common strain identification, drug resistance gene detection can guide clinical medication more accurately, and invalid treatment is avoided.

A primer and probe combination and a kit for nested PCR detection of urinary tract Klebsiella pneumoniae and drug-resistant genes. The primer and probe sequences designed by the invention do not cause cross-amplification of other urinary tract pathogens and can meet the specificity requirements. In addition, exogenous antibiotic interferors gentamicin, tobramycin, ceftazidime, levofloxacin, piperacillin, and nitrofurantoin have no effect on the experimental results, meeting the anti-interference requirements. The prepared kit can simultaneously detect urinary tract Klebsiella pneumoniae and drug-resistant genes, and the detection is convenient, simple, and reliable. Compared with common bacterial species identification, drug-resistant gene detection can more accurately guide clinical medication and avoid ineffective treatment.

The invention provides nitrate reducing bacteria C381 as well as a method and application thereof. The nitrate reducing bacteria C381 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36842; the invention provides a novel oral cavity probiotic nitrate reducing bacteria C381, the nitrate reducing bacteria C381 has an inhibition effect on periodontal disease bacteria, and the nitrate reducing bacteria C381 is sensitive to antibiotics such as erythromycin, piperacillin, minocycline, doxycycline, cefazolin, tetracycline, amikacin, gentamicin, vancomycin and cefoperazone, and can be used for preparing oral cavity probiotics for treating periodontal disease bacteria. And the product has good survival ability in the oral cavity.

The invention discloses a drug-resistant pseudomonas aeruginosa PA-1, the preservation number of the drug-resistant pseudomonas aeruginosa PA-1 in Guangdong Microbiological Culture Collection Center is GDMCC No: 65733. According to the drug-resistant pseudomonas aeruginosa PA-1, drug sensitive experiments show that the drug-resistant pseudomonas aeruginosa PA-1 is resistant to imipenem, ceftazidime, piperacillin, ticarcillin, aztreonam, cefuroxime sodium, cefoxitin and ceftriaxone; a quantitative dilution method experiment shows that the strain is resistant to aztreonam, imipenem, piperacillin / tazobactam, piperacillin, ceftazidime and cefepime; the drug-resistant strain can be used for screening a pseudomonas aeruginosa bacteriostatic preparation, and a new way is provided for development of drugs for treating diseases caused by pseudomonas aeruginosa.

The present invention provides a method for detecting related substances I in piperacillin sodium and tazobactam sodium for injection, belonging to the technical field of drug detection. The detection method comprises preparing a test solution and a reference solution by using piperacillin sodium and tazobactam sodium for injection and a piperacillin reference substance respectively, and then performing high performance liquid chromatography detection. The present invention fully takes into account the requirement of completely separating 11 impurities in piperacillin sodium and tazobactam sodium for injection by adjusting process parameters such as mobile phase and elution procedure, and successfully establishes a method for calculating the content of each impurity, so as to better control the quality of piperacillin sodium and tazobactam sodium for injection.

The invention belongs to the technical field of aquaculture, and particularly relates to separation and identification of bacillus velezensis with an antagonistic effect on nocardia and application of the bacillus velezensis. The bacillus velezensis JZGDFSBV241119 strain is separated from healthy micropterus salmoides culture water, has a good antagonistic effect on nocardia and various pathogenic bacteria, is safe to use, free of residues and hemolytic activity, nontoxic and harmless to fishes and high in high-temperature resistance, acid resistance and salt resistance, and can be applied to the field of fish breeding. And the compound is highly sensitive to 18 antibiotics such as penicillin, ampicillin, piperacillin and the like. The strain can enhance the immune function and enzyme activity of micropterus salmoides and other aquatic animals and reduce the case fatality rate, is beneficial to healthy aquaculture, and is suitable for improving intestinal flora of fishes due to the fact that the strain has the main function of antagonizing pathogenic bacteria.

The application discloses a detection kit for simultaneously determining the concentrations of 12 kinds of antibiotic drugs in trace serum and application, the 12 kinds of antibiotics including vancomycin, meropenem, imipenem, cilastatin, trimethoprim, linezolid, voriconazole, piperacillin, cefoperazone, sulfamethoxazole, cefepime and ceftazidime; the detection kit comprises pretreatment reagents and a liquid chromatography mobile phase; the pretreatment reagents comprise a protein precipitant and purified water; the protein precipitant comprises 50v / v% of methanol and 50v / v% of acetonitrile; the liquid chromatography mobile phase comprises phase A and phase B, the phase A comprises 0.02% of formic acid and 99.98% of water, and the phase B comprises 100% of acetonitrile. The method of the application only needs 10 muL of human serum sample, and can complete the detection within 4 min, can quantitatively determine 12 kinds of antibiotics with significant structural differences, and is simple, rapid, sensitive, accurate and specific.