The present invention relates to a method of simultaneously detecting a plurality of agro-
veterinary drug residues in
bee products. The extracted liquid
trichloroacetic acid or
perchloric acid and the extracted liquid acetate,
phosphate or borate solution are added into a sample; the pH value is controlled between 4.5 and 9.0; the
mixed solution is centrifuged, the filtrate is added into a
solid phase extraction column to be extracted, the extraction column is eluted and dried, the column is washed by
oxalic acid-
methanol solution, the volume of the eluent is defined by the
aqueous solution of
methanol, the eluent is added into liquid
chromatography-
tandem mass spectrometry to be analyzed and tested, the acquired chromatographic peak is contrasted with the known standard chromatographic peak of the
drug, and according to the
retention time and the abundance of the
mass spectrum ions, the specific name of the detected
drug is determined. The method only requires one pre-treatment of the sample, and thus can simultaneously extract 11 classes and more than 60 kinds of
veterinary drug residues, such as sulfonamides, quinolones, macrolides, lincomycins, nitroimidazoles, beta-lactams, tetracyclines, chloromycetins, trinethoprims, chlordimeform, triadimenol and the like, the efficiency of analysis is high, and the detection cost is greatly reduced.