Fluorescent indicator combination, fluorescence array sensor, preparation methods of fluorescent indicator combination and fluorescence array sensor and application

A fluorescent indicator and fluorescence technology, which is applied in the direction of fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of complex processing process, insufficient sensitivity, complex processing, etc., and achieve simple detection process, strong anti-interference ability, and simple production process Effect

Active Publication Date: 2019-07-05
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biggest problem with these methods is that the cost of the required detection equipment is extremely high, and even professional operators are required to complete it, and the sample pretreatment process is complicated, etc., which are not suitable for immediate on-site detection.
[0004] At present, there are many common antibiotic detection methods, among which D.Vega (Analytical and BioanalyticalChemistry 2007, 389, 951–958.) et al. used an electrochemical method to detect four tetracycline antibiotics using electrodes improved by

Method used

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  • Fluorescent indicator combination, fluorescence array sensor, preparation methods of fluorescent indicator combination and fluorescence array sensor and application
  • Fluorescent indicator combination, fluorescence array sensor, preparation methods of fluorescent indicator combination and fluorescence array sensor and application
  • Fluorescent indicator combination, fluorescence array sensor, preparation methods of fluorescent indicator combination and fluorescence array sensor and application

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preparation example Construction

[0047] Further, the preparation method of the present invention comprises the following steps: (1) selecting metal ions with high sensitivity and high selectivity to antibiotics, forming F-CDs-metal ion complexes, and constructing fluorescent indicators; (2) selecting suitable (3) Use a fluorescence spectrometer to record the fluorescence intensity of the fluorescent indicator before and after adding antibiotics to construct a fluorescent array sensor.

[0048] The technical solution, its implementation process and principle will be further explained as follows.

[0049] As an aspect of the technical solution of the present invention, it relates to a fluorescent indicator combination, which includes a variety of full-color fluorescent carbon quantum dots (F-CDs for short) and a variety of metal ions formed under different pH conditions. Carbon quantum dots-metal ion complexes (referred to as F-CDs-metal ion complexes).

[0050] In one embodiment, the fluorescent indicator com...

Embodiment 1

[0112] The construction of embodiment 1 multi-channel fluorescent array sensor

[0113] (1) Screening of fluorescent indicators:

[0114] In order to achieve a clear distinction of multiple classes of antibiotics without cross-interference with each other. When constructing the fluorescent indicator, based on the quenching degree of metal ions to F-CDs, the metal ions were screened, and the metal ion Fe 2+ , Fe 3+ 、Co2+ 、Ni 2+ 、Cu 2+ , Zn 2+ 、Cd 2+ , Hg 2+ , Pb 2+ 、Ag + 、Cr 3+ 、Ce 3+ and Eu 3+ Three kinds of metal ions that have a greater degree of quenching of F-CDs and a stronger ability to bind to antibiotics were screened, namely: Cu 2+ 、Ce 3+ 、Eu 3+ , that constitutes F-CDs-Cu 2+ , F-CDs-Ce 3+ , F-CDs-Eu 3+ Three fluorescent indicators.

[0115] (2) Selection of fixed formula:

[0116] 1) F-CDs (5μg / mL) and Cu 2+ (10 μM) was dissolved in 2 mL of HEPES buffer (10 mM) at pH 7.4, and reacted at room temperature for 5 min to obtain formula 1;

[0117] 2) ...

Embodiment 2

[0127] Embodiment 2 Construction of multi-channel fluorescence array sensor

[0128] (1) Screening of sensing units

[0129] In order to realize the detection and differentiation of four major antibiotics such as tetracyclines, quinolones, β-lactams and aminoglycosamines, 18 response points were screened according to the discrimination effect of different antibiotics, and 8 response points were selected (sensing unit) can realize the distinction of multiple antibiotics, and the specific formulas of the screened 8 response points are as follows:

[0130]

[0131] (2) Add the antibiotic to be tested to the above 8 response points, and after reacting for 5 minutes, record the fluorescence changes before and after adding the antibiotic respectively, and through normalization processing, the obtained data can be analyzed by PCA and HCA to determine different types Discriminative effect of antibiotics.

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Abstract

The invention discloses a fluorescent indicator combination, a fluorescence array sensor, preparation methods of the fluorescent indicator combination and the fluorescence array sensor and application. The fluorescent indicator combination comprises a plurality of carbon quantum dot-metal ion complexes formed by full-color fluorescent carbon quantum dots and various metal ions under conditions ofdifferent pH values. The fluorescent array sensor contains a fluorescent indicator; the sensor comprises a plurality of groups of response points; each group of response points comprise three or moreindependent response points respectively corresponding to excitation wavelengths of 360 nm, 450 nm, and 540 nm; and each of the response points comprises at least one carbon quantum dot-metal ion complex. The fluorescent array sensor of the invention can realize qualitative and semi-quantitative detection of four kinds of antibiotics such as tetracyclines, quinolones, beta-lactams and aminoglycosides; and the preparation process of the sensor is simple; the storage time of the sensor is long; the detection sensitivity of the sensor is high; and the sensor can realize simultaneous differentiation and detection of a plurality of antibiotics.

Description

technical field [0001] The present invention relates to an array sensor used for the detection and differentiation of antibiotics, in particular to a fluorescent indicator combination for detecting and distinguishing multiple types of antibiotics, a multi-channel fluorescent array sensor and a preparation method thereof, as well as its ability to realize multiple types of antibiotics at the same time The application in detection belongs to the technical field of food safety detection. Background technique [0002] Antibiotics, which kill or inhibit the growth of bacteria, have been produced in large quantities around the world over the past few decades and are used to treat various human and veterinary diseases. At present, excessive antibiotics are mostly found in milk, vegetables, grains, surface water and wastewater. Among them, the three classes of antibiotics with high detection frequency were quinolones, tetracyclines and sulfonamides. In order to ensure the qualit...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439Y02A50/30
Inventor 林恒伟乔丽娜钱四化王宇辉
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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