37 results about "Chlamydiaceae" patented technology

The invention discloses a rapid-recognition gene chip for pathogenic bacteria of pneumonia. The gene chip can detect 15 pathogenic bacteria including streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae, and clinically common and difficult-to-culture pathogenic bacteria are contained. In a preparation process of the gene chip, design, screening and verification of probes are performed by adopting 16S rDNA and a specific gene sequence corresponding to each of the pathogenic bacteria, and types of the bacteria in a to-be-detected sample are identified from levels of genus and species simultaneously and respectively. The gene chip has the advantages that the defect that clinical detection of the pathogenic bacteria of pneumonia is not timely and comprehensive at present is overcome, and one novel detection way is provided for early diagnosis and early treatment of patients with pneumonia.

The invention discloses a mycoplasma pneumoniae and chlamydia pneumoniae nucleic acid combined detection kit and an application thereof. A collected sample is split by a cell lysis solution to releasepathogen nucleic acid, and amplification of pathogen nucleic acid fragments is realized through reverse transcription and transcription processes. The amplified RNA product is added into a microporecoated with a coating probe, and a specific probe and an amplification probe are added at the same time, wherein the coating probe can be combined with one end of the specific probe CES to fix the amplified product RNA. One end of the specific probe LES is combined with the RNA product, and the other end of the specific probe LES is combined with the amplification probe to realize signal amplification. The amplification probe marked with multiple biotins is then combined with a streptavidin-HRP enzyme conjugate. Finally, an HRP enzyme chemiluminiscence substrate is added, and detection is carried out on a chemiluminiscence instrument. According to the invention, RNA extraction is not needed, and pollution is not likely to happen in the detection process; and the kit has the advantages of high sensitivity and high specificity, and can be widely applied to mycoplasma pneumoniae and chlamydia pneumoniae nucleic acid combined detection.

The invention provides a fluorescent quantitative PCR detection kit for chlamydia trachomatis in clinic samples, used for assisted diagnosis of chlamydia trachomatis. The kit comprises a PCR solution, a DNA polymerase solution, positive quality control, weakly positive quality control, negative quality control, positive quantitative reference, lysate and protease K, wherein the PCR solution contains forward and reverse primers and the fluorescence probe are specific primers and probe designed for the specific sequence of chlamydia trachomatis and are capable of amplifying a target DNA sequence specifically so as to conveniently and quickly detect chlamydia trachomatis infection in clinic samples. The kit has the characteristics of high specificity and high sensitivity.

The invention relates to a real-time PCR (polymerase chain reaction) method for quintuply detecting target nucleic acid in a nucleic acid extracting solution in a single PCR reaction vessel, which is used for detecting Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in a sample.

Method for detecting nucleic acids which employs a double-stranded oligonucleotide probe containing i) a first probe including a first label moiety, and ii) a second probe partially complementary with the first probe and including a second label moiety capable of interacting with the first moiety when brought in close proximity with each other, the second moiety being a quencher or acceptor of emission of the first moiety. The first or second probe includes a sequence complementary to that of a target nucleotide, and the second or first probe, respectively, includes a sequence complementary to a complement of the target nucleotide sequence of the nucleic acid to be detected. Oligonucleotides for determining Chlamydia trachomatis are also disclosed.

The invention discloses an integrated nucleic acid detection cassette for chlamydia trachomatis, which comprises a cassette body with a cassette cover at the top, a lysis cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the cassette body from top to bottom, the lysis cavity is communicated to the cassette cover, and the second cleaning cavity is communicated to a reaction cavity arranged at the bottom of the outer side of the cassette body; plungers with the plunger hole direction perpendicular to the connecting direction are arranged in the pairwise connecting intervals of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity respectively, and hydrophobic liquid sealing materials are arranged in the plunger holes of the plungers; and an amplification reaction solution is arranged in the reaction cavity, a soluble material layer is arranged in the reaction cavity, and a primer probe mixed solution for Aomp gene of chlamydia trachomatis is embedded in the soluble material layer. According to the invention, the chlamydia trachomatis can be rapidly and integrally subjected to PCR amplification and detection under the condition of relatively low cost, and the problems of poor repeatability, time consumption of a detection method, complicated operation and the like in the prior art are solved.

The present invention relates to primers and probes that can be used in various assays to detect a new strain of Chlamydia trachomatis. The invention further provides for the simultaneous detection of other diseases, especially Neisseria gonorrhoeae.

The invention discloses a codon-optimized Chlamydia trachomatis ctl0286 The gene and its application belong to the technical field of biomedicine. The codon-optimized Chlamydia trachomatis ctl0286 Gene, the sequence of which is shown in SEQ ID NO.1. The present invention realizes the in vitro expression of the Chlamydia trachomatis protein CTL0286 that cannot be expressed in the E. coli system through a codon optimization strategy, and the antibody prepared by using the expressed protein can be used for the identification of the CTL0286 protein; as a specific protein of unknown function in Chlamydia trachomatis, the present invention It can provide the basis for functional research and antibody development of CTL0286 protein, and then provide new targets and drugs for clinical treatment and identification of chlamydia.

The invention is directed to methods, kits, and compositions, for amplifying and detecting Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.

The present invention regards polypeptides capable of eliciting an immunological response that is protective against Chlamydia trachomatis. The polypeptide comprises a first amino acid sequence which has at least 90% homology with the amino acid sequence according to SEQ ID NO: 1 and a second amino acid sequence which has at least 90% homology with the amino acid sequence according to SEQ ID NO: 2. Furthermore, production of these polypeptides and pharmaceutical compositions comprising them are also provided.

The invention provides application of a mimetic peptide compound in preparing a drug for inhibiting intracellular growth of chlamydia trachomatis. The mimetic peptide compound can directly act on HtrA(High Temperature Requirement A) protein of the chlamydia trachomatis and is capable of inhibiting intracellular growth of the chlamydia trachomatis in the level of 10 muM, the quantity and the areaof chlamydia trachomatis inclusion bodies are reduced, and a new path is provided for treating chlamydia trachomatis infection.

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need of two main, and its detection accuracy rate is high, can be up to 98%.

The invention discloses a triple nucleic acid detection kit for neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis. The kit comprises an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction liquid, an enzyme mixed liquid, a triple reaction liquid for neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis, positive control and negative control. The kit disclosed by the invention overcomes the deficiencies that in the prior art, neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis is poor in specificity, lower in sensitivity and the like, effectively prevents pollution, has the advantages of high sensitivity, good specificity, strong repeatability, quick and objective detection result and the like, and has good application prospect for detecting neisseria gonorrhoeae / ureaplasma urealyticum / chlamydia trachomatis.

The present invention relates to a polypeptide that specifically binds to the main outer membrane protein of Chlamydia trachomatis and its application, and discloses for the first time a polypeptide that has binding affinity to the main outer membrane protein of Chlamydia trachomatis; the present invention also provides the in-diagnosis detection of the polypeptide application in medicine, and as a targeting carrier in the diagnosis or treatment of drugs or molecular targeting reagents.

A method of detecting genetic material deriving from Chlamydia trachomatis comprising detection of a specified nucleic acid sequence, optionally using specific primers and probes and optionally in combination with the detection of genetic material deriving from Pectobacterium atrosepticum as an internal control; and related products and kits.

Urogenital infections caused by microbial pathogens are treated by a pharmaceutical composition that treats or prevents a urogenital bacterial infection and that includes 3,3′-diindolylmethane (DIM), or a DIM-related indole, alone or in combination with epigallocatechin-3-gallate (ECGC) and additionally includes one or more antibacterial agents as an option. Such compositions and methods using same are especially useful for treatment or preventing bacterial infections, for example, Ureaplasma urealyticum, Mycoplasma spp, such as M hominis, N. gonorrhea, T. pallidum, Gardnerella spp., and Chlamydia spp., such as Chlamydia trachomatis. Treatment or prevention of any infection may be directed to the vagina, cervix, and / or uterus.