Quintuple fluorescent PCR (polymerase chain reaction) quick and hypersensitive detection kit and application thereof
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 苏州华益美生物科技有限公司
- Publication Date
- 2014-04-02
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Figure 1
Abstract
Description
technical field
[0001] The invention belongs to the technical field of nucleic acid detection. Specifically, the invention relates to a multiplex real-time PCR method capable of simultaneously detecting five pathogenic pathogens such as Chlamydia trachomatis, Neisseria gonorrhoeae, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, It's fast, ultra-sensitive and done in one step. In addition, the present invention also relates to the reagents involved in the above PCR method, such as primers, probes, etc. that are irrelevant to each other, as well as the detection kit used in the above method and the preparation and application of the corresponding detection kit. Background technique
[0002] Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), Mycoplasma hominis (MH), and Mycoplasma genitalium (MG) are the five main pathogens that cause genitourinary system infections, which can cause urethritis, Prostatitis, cervicitis, s...