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A phage capable of lysing multidrug-resistant Pseudomonas aeruginosa and its application in treating infection

A Pseudomonas aeruginosa, multi-drug resistance technology, applied in the direction of medical raw materials derived from viruses/phages, viruses/phages, antibacterial drugs, etc., can solve problems such as Pseudomonas aeruginosa infection

Inactive Publication Date: 2017-04-26
LANZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The purpose of the present invention is to provide a novel biological agent, which uses the unique biological activity of the isolated and purified broad phage to effectively solve the problem of clinical resistance to β-lactams (including carbapenems), aminoglycosides and cephalosporins. Mycocin antibiotics for Pseudomonas aeruginosa infection problems

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  • A phage capable of lysing multidrug-resistant Pseudomonas aeruginosa and its application in treating infection
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  • A phage capable of lysing multidrug-resistant Pseudomonas aeruginosa and its application in treating infection

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Embodiment Construction

[0058] Collection and processing of specimens:

[0059] Take the sewage from Ruide Avenue, Lanzhou City, Gansu Province, 3000ml each time, add 0.333gCaCl 2 . Another 3ml of Pseudomonas aeruginosa standard strain bacterial solution was added, and the next day, it was sterilized by filtration with a 0.22 μm filter membrane. Take 200ml of treated sewage, add 50ml of liquid LB medium, 1.2ml of host bacteria suspension, mix well and place in a 37°C incubator for overnight cultivation. On the next day, 10ml of the amplification solution was taken and centrifuged at 2000r / min for 15mim, and the supernatant was the stock solution containing Pseudomonas aeruginosa phage. Take 0.3ml of the stock solution and 0.6ml of the standard host bacterial solution, mix well and incubate at room temperature for 15 minutes, then add 2ml of semi-solid at 47°C, mix well and pour on solid agar plates to make double plates, incubate at 37°C for 4-6 hours Then observe plaques. If there are plaques, it ...

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Abstract

The invention discloses cleavable multiple-drug resistant pseudomonas aeruginosa bacteriophage and application thereof in infection treatment. The invention discloses cleavable pseudomonas aeruginosa bacteriophage multiple-resistant to beta-lactams, aminoglycosides and cephalosporin antibiotics, which is named as PaP No.106, preserved in China Center for Type CultureCollection(CCTCC) (Budapest treaty preservation unit, Wuhan University, Wuhan City, China) in June 1, 2012 and has the preservation number of CCTCC NO:M2012200. On the basis of the purpose of novel anti infective therapy, the separated and purified cleavable multiple-drug resistant pseudomonas aeruginosa bacteriophage is proved to be capable of cleaving into multi strains of pseudomonas aeruginosa multiple-resistant to beta-lactams (including carbapenems), aminoglycosides and cephalosporin antibiotics in vitro and vivo, so that the cleavable multiple-drug resistant pseudomonas aeruginosa bacteriophage is proved to be feasible in treatment of pseudomonas aeruginosa infection, and a new therapeutic approach for clinical drug resistant bacteria infection is provided.

Description

[0001] Technical field: [0002] The patent of the invention relates to the bacteriophage capable of cracking clinical multiple-resistant β-lactams (including carbapenems), aminoglycosides and cephalosporin antibiotics Pseudomonas aeruginosa and its application in the treatment of clinical drug-resistant strain infection . [0003] Background technique: [0004] Phages were discovered by D. and Twort in 1907 and D. Herelle in 1909. Wherever bacteria exist, corresponding phages may survive. At the beginning of the 20th century, the discovery and clinical application of antibiotics provided an effective way to treat bacterial infections, so that the research on phage biological agents slowed down. But with the increasing threat of multidrug-resistant pathogens, people need to find treatments other than antibiotics to treat infectious problems. Therefore, phage therapy has attracted widespread attention. [0005] Phage is a general term for viruses that infect microorganisms s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
Inventor 马兴铭乔涵刘毕胜柳鹏程吴倩妮张燕燕蔡大敏
Owner LANZHOU UNIVERSITY
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