High-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat

A high-resolution mass spectrometry and simultaneous detection technology, applied in the field of detection and analysis of veterinary drug residues, can solve the problems of complex detection methods and low resolution, and achieve the effects of simple processing methods, large detection throughput and high accuracy

Inactive Publication Date: 2019-12-03
SHANGHAI ANIMAL EPIDEMIC PREVENTION & CONTROL CENT
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Problems solved by technology

[0007] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a high-resolution mass spectrometry method for synchronously detecting 152 kinds of chemical pollutants in livestock and poultry meat. The high-resolution mass spectrometry method should solve the technical problems of low resolution and complex detection methods in the existing methods for detecting chemical pollutant residues in livestock and poultry meat

Method used

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  • High-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat
  • High-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat
  • High-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat

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Embodiment 1

[0039] The present invention provides a high-resolution mass spectrometry method for synchronous detection of 152 kinds of chemical pollutants in livestock and poultry meat. Testosterone, 2-aminoflubendazole, 3-methylquinoxaline-2-hydroxy acid, 4-epitridine, 5-hydroxymebendazole, albendazole sulfone, albendazole sulfoxide, albendazole Dazol, ampicillin or ampicillin, baclofen, bambuterol, butafolamine, betamethasone, bourdesonone or bourtisolone, brobuterol, conbendazole, carbadox, caboterol , ceftiofur, chloramphenicol, chlormadinone acetate, aureomycin, ciprobazole, cimaterol, sibuterol, cinoxacin, ciprofloxacin, clenbuterol, gram Rencerol, isoclenpanate, clenbuterol-methyl or clenbuterol, clenbuterol, clindamycin, clonidine, clorprenaline, cloxacillin, cortisone, cyproheptadine , danofloxacin or danofloxacin mesylate, dexamethasone, difloxacin or difloxacin hydrochloride, dimetridazole / demenidazole, hydroxydemenidazole, dobutamine , Doxycycline / Doxycycline, Enoxacin, Enro...

Embodiment 2

[0082] 1. Extraction

[0083] Weigh 5.00g of the sample (accurate to 0.01g) into a 50mL centrifuge tube, add 20mL of 90% acetonitrile extraction solution, ultrasonically extract for 5min, centrifuge at 8000g for 5min, take the supernatant, and set aside.

[0084] 2. Purification

[0085]Take about 2mL of the stock solution and directly pass it through the reversed-phase solid-phase extraction column, and collect the effluent, take 1.6mL of the effluent and dry it with nitrogen at 40°C to 0.5mL, dilute to 1mL with 0.1% formic acid aqueous solution, vortex, 14000r / min Centrifuge for 5 minutes, take the supernatant and detect it in the positive and negative ion switching mode.

[0086] 3. Screening Analysis

[0087] Screening is carried out through the measured values ​​of retention time and accurate mass number. For example, the deviation between the retention time of the detected chromatographic peak and the retention time in the standard mass spectrum library of chemical pol...

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Abstract

The invention provides a high-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat. The method comprises a step of establishing a chemical pollutant standard quality spectrum library, a sample detection step and a screening analysis step. The method covers 152 target compounds such as sulfanilamides, quinolones, quinoxalines, nitroimidazoles, beta-lactams, tetracyclines, macrolides, chloramphenicols, benzimidazoles, nicotinic agonists, beta-receptor agonists, hormones and the like. The method has the advantages that the sample pretreatment method is simple and convenient; the 152 compounds can be screened and confirmed by one-needle sample injection; the detection flux is large; the accuracy is high; and the method is suitable for simultaneous qualitative confirmation detection of various veterinary drug residues in the livestock and poultry meat.

Description

technical field [0001] The invention belongs to the field of detection and analysis, and relates to a detection and analysis method for veterinary drug residues, in particular to a method for simultaneously determining 152 kinds of veterinary drugs and other veterinary drugs in livestock and poultry meat by using ultra-high performance liquid chromatography-quadrupole / electrostatic field orbitrap high-resolution mass spectrometry Screening methods for compound residues. Background technique [0002] Food safety is a major issue related to the national economy and the people's livelihood. In recent years, with typical incidents such as "clean meat pork", "red heart eggs" and "fast-growing chicken" appearing on the Internet and newspapers, the quality and safety of livestock and poultry products has become a major issue in food quality and safety. An important part of it is increasingly becoming the focus of public attention. Coincidentally, major quality and safety incidents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/06G01N30/72
CPCG01N30/02G01N30/04G01N30/06G01N30/72G01N2030/027G01N2030/062G01N2030/047
Inventor 潘娟严凤曹莹黄士新张婧吴剑平黄家莺顾欣贡松松王博张文刚商军张浩然张亦菲田恺华贤辉姜芹孙冰清徐汀
Owner SHANGHAI ANIMAL EPIDEMIC PREVENTION & CONTROL CENT
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