38 results about "Cyproheptadine" patented technology

The subject invention provides methods for treating symptoms and / or conditions associated with idiopathic hyperhidrosis by using compounds that decrease the activity of serotonin 5-HT2C receptors. Compounds that can ameliorate symptoms of idiopathic hyperhidrosis and associated conditions include 5-HT2C receptor antagonists (i.e., ketanserin, ritanserin, mianserin, mesulergine, cyproheptadine, fluoxetine, mirtazapine, olanzapine, and ziprasidone) as well as 5-HT2C receptor modulators (i.e., inverse agonists, partial agonists, and allosteric modulators).

The invention provides a cyproheptadine semiantigen, an artificial antigen, an antibody, preparation methods of cyproheptadine semiantigen and the artificial antigen, and application of the artificial antigen and the antibody. The cyproheptadine semiantigen has a molecular structure shown as a formula (I), and the cyproheptadine artificial antigen has a molecular structure shown as a formula (II). The titer of a monoclonal antibody obtained from the antigen reaches 1:300000, and the 50% inhibiting concentration is 0.20 mu g / L. The generated antibody is high in specificity, high in sensitivity and high in accuracy. The antigen and the antibody have wide application prospect on detection of cyproheptadine residue in food.

The present invention discloses a cyproheptadine hapten, a corresponding artificial antigen and a corresponding monoclonal antibody, and further discloses preparation method and applications of the cyproheptadine hapten, the corresponding artificial antigen and the corresponding monoclonal antibody. According to the present invention, the cyproheptadine hapten is the product represented by a formula I, and the product represented by the formula I and carrier protein are connected to obtain the cyproheptadine antigen, wherein the cyproheptadine antigen can be used for preparing the cyproheptadine specific antibody; the preparation method has characteristics of simpleness, feasibility, low cost, and high hapten yield; and after the cyproheptadine artificial antigen of the present invention is used to immunize animals, the cyproheptadine-specific antibodies can be produced, wherein the antibodies can be used for preparing the enzyme-linked immunosorbent assay kit for detecting the cyproheptadine residue, and the kit has advantages of simpleness, rapidness, large sample treatment amount, high sensitivity, strong specificity, and the like.

The present invention relates to a naphthol / graphene / bipyridinium electrochemiluminescence sensor preparation method and an application thereof to determining cyproheptadine hydrochloride. The methodis characterized in that: a Nafion membrane method is used to fix graphene and bipyridylium on a gold electrode, and electrochemiluminescence of cyproheptadine hydrochloride is detected; the result isa high voltage of 850 V of a photomultiplier tube and a scan rate of 0.1 V / s; a boric acid buffer solution with pH=7.5 is used; the luminescence intensity of the cyproheptadine hydrochloride standardin the concentration range of 1x10-7 to 6x10-5 mol / L satisfies the linearity; and the adding standard recovery of cyproheptadine hydrochloride is between 99.7% and 104.9%. The method provided by thepresent invention has good sensitivity and stability for testing cyproheptadine hydrochloride, and has a good effect; and compared with the bare electrode, by using the method, the utilization efficiency of bipyridylium can be improved, environmental pollution can be reduced, and a test cot can be saved, and the method has important significance for expanding the application of electrochemiluminescence in drug analysis.

The invention provides a preparation method of enzyme-linked immunosorbent assay (ELISA) kit for detecting cyproheptadine hydrochloride. The kit is sensitive, accurate and rapid in detection, simple and convenient to operate, high in specificity and suitable for detecting samples in large batch, and comprises an ELISA plate coated with a cyproheptadine hydrochloride antigen, a cyproheptadine hydrochloride standard substance, a cyproheptadine hydrochloride enzyme-labeled antibody working solution, a substrate solution A, a substrate solution B, a stop solution and a concentrated scrubbing solution. According the principle of the kit, an ELISA reaction is performed through direct solid-phase competition; an extracted sample and the enzyme-labeled antibody working solution are added into a corresponding ELISA hole; after a period of incubation, the ELISA plate is scrubbed, and the substrate solution A and the substrate solution B are added; under the enzymatic action, a blue color appears in the ELISA hole; after the stop solution is added, the color changes from blue to yellow, and the color depth has an inversely proportional relationship with the content of cyproheptadine hydrochloride in the standard substance or the sample. The principle can be directly utilized for detecting the content of cyproheptadine hydrochloride in a swine urine sample.

A hybridoma cell line of secreting cyproheptadine monoclonal antibodies with a preservation number of CGMCC No. 14699 belongs to the field of food safety immunological detection. BALB / c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with cyproheptadine complete antigen without adjuvant; the spleen cells from BALB / C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three sub-clones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to cyproheptadine (value of IC50 is 0.37 ng / ml), being suitable for detection of cyproheptadine in food.

The invention provides a cyproheptadine hydrochloride immune colloidal gold detection card and a preparation method thereof, and relates to the technical field of beta-adrenoreceptor agonist detection. According to the present invention, the test paper strip in the detection card housing comprises a PVC glue plate, a sample pad, a colloidal gold conjugation pad, a coating membrane and a water absorbing pad, wherein the colloidal gold membrane is a glass cellulose membrane containing cyproheptadine hydrochloride monoclonal antibody, the coating membrane is a nitrocellulose membrane and is provided with a T line and a C line, a cyproheptadine hydrochloride protein conjugate is coated on the T line, and a goat anti-mouse IgG antibody is coated on the C line; and the detection card and the detection method are effectively used for cyproheptadine hydrochloride detection, and advantages of convenience, rapidness and accurate result are provided.

The invention provides treating cachexia and eating disorders in humans and veterinary animals by administering a composition including: (i) cyproheptadine (CYP); and (ii) a cannabis compound selected from the group of cannabidiol (CBD) and mixtures of CBD and up to 50% tetrahydrocannabinol (THC).

The invention discloses a dandruff-removal composition, which comprises the following components: ketoconazole, clobetasol propionate, vitamin B6, dimethyl sulfoxide, octadecanol, glycerin monostearate, glycerol distearates, cyproheptadine, 2,6-butylated hydroxytoluene and glycerol. Verified by experiments performed by the applicant, the dandruff-removal composition disclosed by the invention is obvious in effect of completely removing dandruffs when being used for dandruff-removal hair care. Moreover, the holding time is long, and dandruffs cannot relapse in at least two years.

The invention discloses an external ointment for treating internal and external hemorrhoids as well as burns and scalds. The ointment is prepared from the following Chinese and western medicines and auxiliary materials, and obtained by crushing subprostrate sophora, typhonium giganteum, fructus cnidii, fructus kochiae, caesalpinia cucullata, cyproheptadine, promethazine, borneol and B2, adding powdery vanishing cream, and stirring. The ointment has the functions of diminishing inflammation, easing pain, killing bacteria, stopping bleeding, astringing, and resisting irritability, acts on the effects of dispersing blood stasis and promoting granulation, and accelerates wound healing, as well as is wide in adaptation diseases, convenient to use, safe and free from side effects. The ointment is capable of curing burns and scalds, malignant sores and anabrosis, internal and external hemorrhoids, wound joint swelling, perianal abscess, allergic dermatitis, mosquito bite, herpes zoster, dankness tinea and dental ulcer.

The invention discloses allergy-removal rhinitis cream which is prepared from, by weight, 8-10% of astragalus membranaceus, 8-10% of codonopsis pilosula, 10-15% of scutellaria baicalensis, 3-5% of lumbricus, 3-5% of as arum, 3-5% of honeysuckle, 3-5% of menthol, 3-5% of radix aconiti carmichaeli and the balance finished product western medicine supplementary materials; the finished product western medicine supplementary materials comprise ketotifen, cyproheptadine, prednisone, chlortrimeton and magnesium sulfate; the traditional Chinese medicine is crushed and ground in proportion, the western medicine supplementary materials are crushed and ground in proportion, the traditional Chinese medicine ingredients and the western medicine supplementary materials are stirred and mixed, polyving akohol, sodium methyl cellulose and glycerinum are added and blended into a paste shape, and the allergy-removal rhinitis cream is obtained. The Chinese herbal medicine has the effects of clearing away heat and dispelling wind-evil, dispelling cold to dredge main and collateral channels, tonifying the lungs and the spleen, and nourishing the kidney and supplementing the yang; the finished product western medicine supplementary materials have the functions of inhibiting exudation of inflammation, lowering the permeability of blood capillary, resisting to histamine, acetyl choline and 5-hydroxytryptamine are achieved, cytomembranes of mast cells can be stabilized, and releasing of anaphylactic mediators is inhibited.

The invention provides a composite liniment for curing acne and comedo, which comprises the following raw materials by weight ratio: 0.5 to 2.0 parts of tetracycline, 0.3 to 2.0 parts of erythrocin, 0.4 to 2.0 parts of cyproheptadine, 0.3 to 1.0 part of pennyroyal, 0.1 to 1 part of borneol, 1.0 to 2.5 parts of vitamin B6, 3 to 10 parts of radix notoginseng and 100 parts of plant extractive. The preparation method of the liniment comprises the following steps: weighing tetracycline, erythrocin, cyproheptadine, vitamin B6 and borneol; adding the plant extractive; stirring uniformly; adding pennyroyal; stirring uniformly; homogenizing for 10 to 30 minutes by the ultrasonic wave at the temperature of 0 DEG C to 10 DEG C; and split-charging to obtain the finished product. The liniment conforms to the chemical property of the skin, facilitates the performance of the physiological function of the skin, plays a role in not only curing but also protecting the skin, has multiple curative effects compared with the simple application of tetracycline and erythrocin, and reduces the dry and tension senses of the skin when in administration, furthermore, the liniment has low irritation and is easily accepted by the patients, thereby the application prospect is wide.

The invention provides treating cachexia and eating disorders in humans and veterinary animals by administering a composition including: (i) cyproheptadine (CYP); and (ii) a cannabis compound selected from the group of cannabidiol (CBD) and mixtures of CBD and up to 50% tetrahydrocannabinol (THC).

The invention discloses a medicine composition for treating tuberculous pleurisy, a method for preparing the medicine composition and application thereof. The medicine composition comprises, by weight, 1-3 parts of artemisinin, 3-7 parts of cyproheptadine, 15-23 parts of taurine and 11-19 parts of cefuroxime. The method includes mixing and grinding the cyproheptadine and the cefuroxime with each other, adding deionized water into the cyproheptadine and the cefuroxime and stirring and treating the deionized water, the cyproheptadine and the cefuroxime at the temperature of 66 DEG C for 48-50 min to obtain mixtures A; mixing the taurine and the mixtures A with one another, stirring and treating the taurine and the mixtures A at the temperature of 60 DEG C for 23-25 min, adding the artemisinin into the taurine and the mixtures A to obtain other mixtures, carrying out ultrasonic treatment on the other mixtures at the temperature of 43 DEG C for 33 min, then stirring the other mixtures at the temperature of 55 DEG C until the other mixtures are dried and granulating the other mixtures to obtain the medicine composition. The medicine composition, the method and the application have the advantages that the medicine composition can be used for treating the tuberculous pleurisy, is high in cure rate, short in treatment time, free of toxic and side effects or allergic reaction and low in manufacturing cost and dosage, can become effective fast and is a perfect medicine, obvious treatment effects can be realized, and the tuberculous pleurisy is low in recurrence rate.

The invention discloses an orally-taken preparation for treating urticaria and a preparation method thereof. The orally-taken preparation for treating urticaria is prepared from, by weight, paeonol, lentinan, betulinic acid, tannin, volatile oil, beta-sitosterol, tea saponin, cyproheptadine, flavonoid glycosides, colocasia tonoimo extract, boehmeria siamensis craib extract, lycopodium phlegmaria L. extract, vitamin C and vitamin P. The orally-taken preparation combines western medicine and effective constituents of the traditional Chinese medicine extract, has the effects of dispelling wind, relieving itching, clearing away heat and toxic materials, tonifying the spleen, eliminating dampness and resisting to bacteria, infection and allergy, meanwhile can regulate immunocompetence of the human body and lower the side effects of medicine, can be used for treating urticaria which attacks repeatedly and is remarkable in treatment effect.

The invention belongs to the technical field of African swine fever treatment, and particularly relates to application of a compound cyproheptadine hydrochloride in preventing or treating African swine fever. The invention finds that the compound cyproheptadine hydrochloride can significantly inhibit ASFV replication. It is found that cyproheptadine hydrochloride not only inhibits transcription and protein expression of a target protein D1133L, but also can reduce transcription and protein expression levels of p30 and p72 and prevent viruses from invading host cells, and can be used for inhibiting early and late stage infection of ASFV. Therefore, the compound cyproheptadine hydrochloride can be used for preventing or treating African swine fever.

The invention provides a histamine receptor inhibitor and derivatives thereof in the preparation of anti-Zika virus drugs. The histamine receptor inhibitor is preferably one or more of mebhydrolin napadisylate, ketotifen fumarate, flunarizine hydrochloride, cyproheptadinehydrochloride, JNJ-7777120, desloratadine, brompheniramine maleate, brompheniramine maleate, chlorpheniramine maleate and cetirizine hydrochloride. The invention brings forward for the first time that the histamine receptor inhibitor and its derivatives have clear anti-Zika virus effect and can significantly inhibit the expression level of Zika virus protein and Zika virus RNA. The invention also screens out histamine receptor inhibitors with better inhibitory effect on Zika virus, including mebhydrolin napadisylate, cyproheptadinehydrochloride, JNJ-7777120, desloratadine, etc., whose inhibition effect on Zika virus at a concentration of 10 uM is equivalent to inhibition effect of ribavirin at a concentration of 40 uM.The histamine receptor inhibitor and the derivatives can be used as good candidate drugs for anti-Zika virus drugs.

The invention provides a chemiluminescence immunodetection kit for detecting cyproheptadine, and belongs to the field of immunological detection. The kit consists of a non-transparent white elisa plate coated with a cyproheptadine-carrier protein conjugate, a cyproheptadine standard product, a cyproheptadine-peroxidase labeled antibody working solution, a luminous substrate solution, a concentrated sample diluting solution and a concentrated cleaning solution. The cyproheptadine-carrier protein conjugate is prepared from cyproheptadine and carrier protein through a mixed anhydride method or a carbodiimide method, and the concentrated cleaning solution comprises 0.05% Tween-20. Compared with the conventional enzyme linked immunosorbent assay method, the kit provided by the invention is higher in sensitivity, short in detection time and low in cost, and can be used for detecting the yproheptadine residual amount of samples of animal urine, blood samples, tissue, internal organs and the like.

The invention discloses a time-resolved fluorescence immunoassay kit for detecting cyproheptadine. The time-resolved fluoroimmunoassay detection kit for detecting cyproheptadine is composed of a porous coating plate, a buffer solution, a cyproheptadine standard substance, a cyproheptadine antibody freeze-dried product, a europium-labeled goat anti-mouse antibody, a washing solution and an enhancement solution. A detection method of the time fluorescence immunoassay kit for detecting cyproheptadine comprises the following steps: (1) preparing an immunogen; (2) preparing a coated antigen; (3) preparing a monoclonal antibody; and (4) pretreating and detecting a sample. The kit and the method are short in detection time, high in average recovery rate, simple in sample pretreatment, wide in application and low in detection cost, can be operated and detected on site, and have the advantages of high detection specificity, small intra-batch and inter-batch difference, high sensitivity, simplicity and quickness in operation, particular suitability for detection of large-batch samples and the like.

The invention relates to an electrochemiluminescence detection method of cyproheptadine hydrochloride. According to the method, polyacrylonitrile, polyvinylpyrrolidone and phosphoric acid are used asraw materials, N, P-PCNF is successfully prepared by adopting a method of combining electrostatic spinning with high-temperature carbonization, a glassy carbon electrode is modified to be used as a working electrode, and an electrochemiluminescence method is used for detecting the content of cyproheptadine hydrochloride in a sample. Experiments show that the N, P-PCNF has the advantages of large specific surface area, high conductivity, multiple edge defects, multiple active sites, high electron transfer speed and the like, and has good electrocatalytic activity on cyproheptadine hydrochloride. Under the optimal experimental conditions, the concentration of cyproheptadine hydrochloride and the luminous intensity have a good linear relationship. The method for detecting cyproheptadine hydrochloride has the characteristics of high sensitivity, low detection limit and wide detection range, and can be used for detecting actual samples.

The invention discloses a medicine composition for treating tuberculous pleuritis, and a preparation method and application thereof. The medicine composition is prepared from the following raw materials in parts by weight: 1 to 3 parts of allicin, 3 to 7 parts of cyproheptadine, 15 to 23 parts of radix puerariae flavone, and 11 to 19 parts of cefuroxime. The preparation method comprises the steps of mixing and grinding the cyproheptadine and the cefuroxime, adding deionized water, stirring for 48 to 50 min at the temperature of 66 DEG C, and preparing a mixture A; mixing the radix puerariae flavone and the mixture A, stirring for 23 to 25 min at the temperature of 60 DEG C, adding the allicin, then carrying out ultrasonic treatment for 33 min at the temperature of 43 DEG C, stirring at the temperature of 55 DEG C until dry, and pelleting. The medicine composition for treating tuberculous pleuritis provided by the invention is remarkable in treatment effect, high in cure rate, short in treatment time, fast in effect, free of toxic and side effect and anaphylactic reaction, high in cure rate, low in recurrence rate, low in cost, and less in dosage, and is a more ideal medicine.

The invention provides a composite liniment for curing acne and comedo, which comprises the following raw materials by weight ratio: 0.5 to 2.0 parts of tetracycline, 0.3 to 2.0 parts of erythrocin, 0.4 to 2.0 parts of cyproheptadine, 0.3 to 1.0 part of pennyroyal, 0.1 to 1 part of borneol, 1.0 to 2.5 parts of vitamin B6, 3 to 10 parts of radix notoginseng and 100 parts of plant extractive. The preparation method of the liniment comprises the following steps: weighing tetracycline, erythrocin, cyproheptadine, vitamin B6 and borneol; adding the plant extractive; stirring uniformly; adding pennyroyal; stirring uniformly; homogenizing for 10 to 30 minutes by the ultrasonic wave at the temperature of 0 DEG C to 10 DEG C; and split-charging to obtain the finished product. The liniment conformsto the chemical property of the skin, facilitates the performance of the physiological function of the skin, plays a role in not only curing but also protecting the skin, has multiple curative effects compared with the simple application of tetracycline and erythrocin, and reduces the dry and tension senses of the skin when in administration, furthermore, the liniment has low irritation and is easily accepted by the patients, thereby the application prospect is wide.

The invention provides a compound liniment for treating acne rosacea, which consists of the following raw materials by weight percentage: cyproheptadine, vitamin B6, sulfur, mercurous chloride, radix sileris, clove oil, borneol and the like. The compound liniment accords with the chemical characteristics of skin, is advantageous for giving play to physiological functions of the skin, is safe and reliable for treating the acne rosacea, can quickly deliver drugs to a pathologic change part to diminish inflammation, kill mites and sterilize powerfully, and play a direct action on the root of niduses to thoroughly dispel symptoms of itching, red, blains and the like of the acne rosacea. By combining with DS reparative factors, the compound liniment can repair the microcirculation of the skin in a short time, regulate sebum secretion metabolism, and reconstruct the immunologic function of normal skin to thoroughly cure the acne rosacea and prevent recurrent attacks; and in the using process, the compound liniment reduces the dryness and tension of nose skin, has small acrimony, can be accepted by patients easily, and has broad application prospect.

The invention discloses a hydrochloric acid cyproheptadine cream which is prepared by the following ingredients, cyproheptadine hydrochloride, corn mint, stearic acid, glyceryl monostearate, triethanolamine, glycerin, white petrolatum and purified water. Wherein, hydrochloric acid cyproheptadine is antihistamine and used for relieving the allergic reaction caused by the histamine; the corn mint is distributed in substrate with the form of pellets and melts under the body surface temperature, so as to have cool, comfortable aroma and good psychological curative effect. The hydrochloric acid cyproheptadine cream is used for the treatment of allergic dermatitis contact dermatitis, pruritus and eczema. The hydrochloric acid cyproheptadine cream has the advantages of novel substrate appearance, good stability, convenient carrying and storage.

Production of 10,11-dihydro-dibenzo(b, f)chedder-10-ketone compound is carried out by taking o-nitrophenylacetic acid as initial raw material, reducing, diazotizing, coupling with phenyl-sulfhydrate derivatives, acylating, and cyclizing to obtain (I). It achieves simple process, gentle reaction and more yields.

The invention discloses a dermatosis medicine composed of several Chinese and western medicines, and relates to the field of dermatosis medicines.The dermatosis medicine is characterized in that 1, the medicine is prepared, and tablets including vitamin b1, vitamin b2, vitamin b12, vitamin b6, prednisone, compound pyrimidine, ground rice, vitamin c, chlortrimeton and cyproheptadine are prepared; the ointment comprises fluocinonide vitamin 6 cream and white vaseline; the traditional Chinese medicines comprise borneol and camphor; 2, medicine treatment, wherein the used solid medicine is smashed, and then the smashed medicine is filtered through a screen; 3, adding the prepared vaseline into a mixing barrel; 4, the used medicines are sequentially added into the mixing barrel according to the proportion; 5, the medicine in the mixing barrel is stirred till the color of the medicine is uniform, the skin disease can be treated through the prepared medicine, meanwhile, the irritation to the treatment position can be reduced through the introduced borneol and camphor, and meanwhile damage to the human body is reduced.