The present invention discloses a cyproheptadine hapten, a corresponding artificial antigen and a corresponding monoclonal antibody, and further discloses preparation method and applications of the cyproheptadine hapten, the corresponding artificial antigen and the corresponding monoclonal antibody. According to the present invention, the cyproheptadine hapten is the product represented by a formula I, and the product represented by the formula I and carrier protein are connected to obtain the cyproheptadine antigen, wherein the cyproheptadine antigen can be used for preparing the cyproheptadine specific antibody; the preparation method has characteristics of simpleness, feasibility, low cost, and high hapten yield; and after the cyproheptadine artificial antigen of the present invention is used to immunize animals, the cyproheptadine-specific antibodies can be produced, wherein the antibodies can be used for preparing the enzyme-linked immunosorbent assay kit for detecting the cyproheptadine residue, and the kit has advantages of simpleness, rapidness, large sample treatment amount, high sensitivity, strong specificity, and the like.