Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof

The technology of cyproheptadine hydrochloride and colloidal gold is applied in the field of immunocolloidal gold detection card of cyproheptadine hydrochloride and its preparation field, and the effects of accurate results and simple method are achieved.

Inactive Publication Date: 2016-01-27
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, the object of the present invention is to provide a kind of immune colloidal gold detection card of cyproheptadine hydrochloride and preparation method thereo...

Method used

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  • Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof
  • Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof
  • Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] figure 1 , figure 2 Shown embodiment: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold binding pad, and the colloidal gold binding pad is coated with a monoclonal antibody colloidal gold marker; 10 is a coating film, i.e. nitrocellulose Plain membrane, the nitrocellulose membrane coated with cyproheptadine hydrochloride-BSA and goat anti-mouse IgG; 11 is a water-absorbent pad, made of water-absorbent materials such as filter paper.

[0021] On one end (sample end) of the PVC rubber plate 9, the sample pad 7 and the bonding pad 8 are adhered, and the sample pad 7 and the bonding pad 8 are in a side-by-side structure.

[0022] A nitrocellulose membrane 10 is adhered in the middle of the PVC rubber sheet 9 . Goat anti-mouse IgG quality control line 3 and cyproheptadine hydrochloride-BSA detection line 2 are set on the nitrocellulose membrane 10 .

[0023] At the other end of the PVC rubber sheet 9, a water-absorbing pad 11 is adhered. ...

Embodiment 2

[0025] The preparation of the cyproheptadine hydrochloride colloidal gold detection card is specifically realized by the following steps:

[0026] Preparation of colloidal gold solution: Take a 1L Erlenmeyer flask, add 495mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 (0) 5mL, be prepared into 500mL0.01% chloroauric acid aqueous solution, add 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) solution 5-7mL, continue to stir and heat, when the color of the solution turns into transparent purple red completely, stop heating after maintaining 5min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0027] Antibody pretreatment: Centrifuge the cyproheptadine hydrochloride antibody to be labeled at 1000r / min, 4°C for 20min, take the supernatant, and dilute to 1mg / mL with 0.01mol / LPBS; or dilute to 1mg with 0.01mol / LPBS / ml, pass through a 0.22μm filter membrane;

[0028] Preparation of colloidal gold markers: ...

Embodiment 3

[0034] The minimum detection limit test of cyproheptadine hydrochloride

[0035] Accurately weigh 0.500g of cyproheptadine hydrochloride antigen, dissolve it in ultrapure water and dilute to 100mL, marked as A 1 solution, take A 1 Add 1mL of the solution to the volumetric flask and add ultrapure water to 1000mL to form A 2 solution, take A 2 Add 1mL of the solution to the volumetric flask and add ultrapure water to 1000mL to form A 3 solution, the concentration of A3 solution is 5ng / mL (5ppb). take A 3 Put 1mL of the solution in 4 test tubes, and label them 1~4 respectively, add 9mL, 4mL, 1mL, 0mL of ultrapure water to the test tubes respectively, and prepare the cyproheptadine hydrochloride antigen solution with concentrations of 0.5ng / mL, 1ng / mL, 2.5ng / mL, 5ng / mL; take another empty test tube, add 5mL ultrapure water, and mark it as No. 5 test tube. Take the liquid in the above-mentioned No. 1-5 test tube as a sample and add it dropwise to the cyproheptadine hydrochlori...

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Abstract

The invention provides a cyproheptadine hydrochloride immune colloidal gold detection card and a preparation method thereof, and relates to the technical field of beta-adrenoreceptor agonist detection. According to the present invention, the test paper strip in the detection card housing comprises a PVC glue plate, a sample pad, a colloidal gold conjugation pad, a coating membrane and a water absorbing pad, wherein the colloidal gold membrane is a glass cellulose membrane containing cyproheptadine hydrochloride monoclonal antibody, the coating membrane is a nitrocellulose membrane and is provided with a T line and a C line, a cyproheptadine hydrochloride protein conjugate is coated on the T line, and a goat anti-mouse IgG antibody is coated on the C line; and the detection card and the detection method are effectively used for cyproheptadine hydrochloride detection, and advantages of convenience, rapidness and accurate result are provided.

Description

technical field [0001] The invention relates to the technical field of animal source food additive detection, in particular to an immune colloidal gold detection card for cyproheptadine hydrochloride and a preparation method thereof. Background technique [0002] Cyproheptadine hydrochloride (Cyproheptadinehydrochloride, molecular formula: C 21 h 21 N·HCl) is an antihistamine with anti-5-HT, blocking H1 receptor and anticholinergic effects, mainly used in the treatment of urticaria, eczema, contact dermatitis, itchy skin, allergic rhinitis, etc. Its common side effects are mild to moderate drowsiness, dizziness, fatigue, dry mouth and so on. Consumption of animal-derived products containing clonidine hydrochloride and cyproheptadine hydrochloride residues is harmful to human health. The Ministry of Agriculture issued Announcement No. 1519 on December 27, 2010, prohibiting the use of clonidine hydrochloride and cyproheptadine hydrochloride in feed and animal drinking water...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 洪霞薛永来
Owner JIANGSU WISE SCI & TECH DEV
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