This utility model discloses a sample pretreatment device for rapid microbial detection, mainly relating to sample pretreatment equipment. It includes a heater, a heating plate, and an auxiliary structure. A control button is installed on one side of the heater. The upper surface of the heater is fixed to the heating plate via the auxiliary structure. A sample cup is placed on the upper surface of the heating plate. A stirrer is installed on one side of the heater. An adjustment structure is provided on the upper surface of the heating plate. The adjustment structure includes a circular frame, which is fixedly connected to the heating plate. A fixing groove is formed on the inner wall of the circular frame, and the fixing groove is slidably connected to the sample cup. The beneficial effects of this utility model are: it can conveniently fix the sample cup, preventing sample overflow or leakage due to shaking during stirring, thereby ensuring the stability and safety of the sample during stirring, significantly improving the accuracy of operation, and achieving the
advantage of making the sample more stable during stirring.