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Naphthol/graphene/bipyridinium electrochemiluminescence sensor preparation method and application thereof to determining cyproheptadine hydrochloride

A cyproheptadine hydrochloride, pyridine ruthenium electrolysis technology, which is applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis by making materials undergo chemical reactions, etc., can solve the problems of low sensitivity, narrow linear range of measurement, cumbersome operation, etc. problems, achieve good sensitivity and stability, save test costs, and improve utilization efficiency

Inactive Publication Date: 2019-10-15
LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the detection of cyproheptadine hydrochloride generally adopts high performance liquid chromatography, but this method is not only cumbersome to operate, but also generally has shortcomings such as narrow linear range of determination, high detection limit, low sensitivity, etc. Rapid, accurate and efficient determination of pyridine
The study utilizes the sensitization effect of cyproheptadine hydrochloride on bipyridyl ruthenium, and establishes a new method for the determination of cyproheptadine hydrochloride by electrochemiluminescence. detection

Method used

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  • Naphthol/graphene/bipyridinium electrochemiluminescence sensor preparation method and application thereof to determining cyproheptadine hydrochloride
  • Naphthol/graphene/bipyridinium electrochemiluminescence sensor preparation method and application thereof to determining cyproheptadine hydrochloride
  • Naphthol/graphene/bipyridinium electrochemiluminescence sensor preparation method and application thereof to determining cyproheptadine hydrochloride

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Effect test

Embodiment 1

[0025] Embodiment 1: a kind of preparation method of naphthol / graphene / bipyridyl ruthenium electrochemiluminescence sensor, comprises the following steps:

[0026] (1) Electrode pretreatment: put the gold electrode on the buckskin with A1 2 o 3 The powder is polished to a mirror surface, rinsed with water, then ultrasonically cleaned with water, and dried at room temperature; put the gold electrode, counter electrode and reference electrode into the luminescence cell of the MPI-E electrochemiluminescence analysis system, and use a micropipette Add 100 µL of sulfuric acid with a concentration of 0.1 mol / L to the luminescence pool, and 200 µL of phosphate buffer solution with pH=8.5 for detection until a stable luminescence curve is obtained. After pretreatment, put it on the reagent rack for use;

[0027](2) Preparation of modifiers: Mix 75 µL of naphthol (Np), 1.5 mL of isopropanol, and 6 mL of double distilled water, put them into an ultrasonic cleaner for 3 minutes, and obt...

Embodiment 2

[0029] Embodiment 2: a kind of naphthol / graphene / bipyridyl ruthenium electrochemiluminescence sensor as described in embodiment 1 is measuring the application of cyproheptadine hydrochloride, comprising the following steps:

[0030] (1) The three-electrode system of the electrochemical workstation was used for testing, the Ag / AgCl electrode was used as the reference electrode, the platinum wire electrode was used as the counter electrode, and the prepared naphthol / graphene / bipyridyl ruthenium electrochemiluminescence sensor was used as the working electrode , the high voltage of the photomultiplier tube is set to 850V, and the scan rate is set to 0.1V / s;

[0031] (2) In 200 µL boric acid buffer solution with pH=7.5, detect different concentrations of cyproheptadine hydrochloride standard solution by electrochemiluminescence system, record the generated electrochemiluminescence signal intensity, and draw the working curve;

[0032] (3) The cyproheptadine hydrochloride sample so...

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Abstract

The present invention relates to a naphthol / graphene / bipyridinium electrochemiluminescence sensor preparation method and an application thereof to determining cyproheptadine hydrochloride. The methodis characterized in that: a Nafion membrane method is used to fix graphene and bipyridylium on a gold electrode, and electrochemiluminescence of cyproheptadine hydrochloride is detected; the result isa high voltage of 850 V of a photomultiplier tube and a scan rate of 0.1 V / s; a boric acid buffer solution with pH=7.5 is used; the luminescence intensity of the cyproheptadine hydrochloride standardin the concentration range of 1x10-7 to 6x10-5 mol / L satisfies the linearity; and the adding standard recovery of cyproheptadine hydrochloride is between 99.7% and 104.9%. The method provided by thepresent invention has good sensitivity and stability for testing cyproheptadine hydrochloride, and has a good effect; and compared with the bare electrode, by using the method, the utilization efficiency of bipyridylium can be improved, environmental pollution can be reduced, and a test cot can be saved, and the method has important significance for expanding the application of electrochemiluminescence in drug analysis.

Description

technical field [0001] The invention relates to the preparation and application of an electrochemiluminescence sensor, in particular to a preparation method of a naphthol / graphene / bipyridyl ruthenium electrochemiluminescence sensor and its application in the determination of cyproheptadine hydrochloride. Background technique [0002] The English name of cyproheptadine hydrochloride is Cyproheptadine, HCL, and its different names are diphenylcyclo, heptadine, mitongidine, etc., and its molecular formula is C 21 h 22 CIN is a white tablet, and the excipients are calcium sulfate, starch, dextrin, sodium starch glycolate, and magnesium stearate; studies have shown that cyproheptadine hydrochloride is H 1 Receptor antagonist, is a hexahydropyridine antihistamine, its antihistamine effect is stronger than chlorpheniramine and promethazine, currently mainly used for urticaria, eczema, allergic and contact dermatitis, skin itching, etc. Anaphylaxis is a class of anticoagulant and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30G01N27/416
CPCG01N21/76G01N27/308G01N27/416
Inventor 罗应李彦青李利军李辉程昊孔红星
Owner LUSHAN COLLEGE OF GUANGXI UNIV OF SCI & TECH
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