The invention provides a purification method of high-purity
teicoplanin refined
powder, which comprises the following steps: centrifuging a
teicoplanin fermentation liquid under alkaline conditions to remove solids in the
fermentation liquid, regulating the filtrate to a
neutral state, adsorbing and eluting with a macroporous adsorbent resin to obtain a
teicoplanin eluate with the concentration of 65% above, and filtering by gel
chromatography to obtain a 80% teicoplanin eluate; concentrating the eluate, adding
acetone to precipitate, and centrifuging to obtain a milk white teicoplanin crude product; and dissolving the crude product with a right amount of water, adding the sample to a
chromatographic column filled with reversed phase
silica gel, carrying out
gradient elution with an
acetonitrile-water
mixed solution to obtain a high-purity teicoplanin eluate, adding
activated carbon for decolorization, filtering, cooling to precipitate, removing the residual impurities, adding
acetone to the clear solution to completely precipitate, and carrying out
infrared drying on the wet product to obtain the teicoplanin pure product. The method has the advantages of simple technique, reliable quality, high purity of the teicoplanin refined
powder and controllable single-component content, and is suitable for industrial large-scale production of high-purity teicoplanin.