The invention relates to a preparation method of single
teicoplanin components, which belongs to the field of preparation of medicaments with microbial ingredients. The method comprises the following steps of: decolorizing
teicoplanin fermentation liquor through macroporous decolorizing resin, and absorbing the
teicoplanin fermentation liquor through macroporous adsorption resin, then desorbing the teicoplanin
fermentation liquor, and concentrating the teicoplanin fermentation liquor to obtain a crude teicoplanin extract including the six components of TA2-1, TA2-2, TA2-3, TA2-4, TA2-5 and TA3-1; and dissolving the crude extract in water, and performing
chromatographic separation on the dissolved crude extract through
polymer microspheres to obtain the six single components, namely TA2-1, TA2-2, TA2-3, TA2-4, TA2-5 and TA3-1, of teicoplanin with high purity. According to the
separation method of the single teicoplanin components, the novel
chromatography media-
polymer microspheres are adopted to simultaneously separate the six single components from the crude teicoplanin extract; and the method is simple in technology, can be used for disposing a large quantity of samples, consumes less
solvent, can be used for preparing the single teicoplanin components in a large scale, and provides samples for intensive study on
pathology,
toxicology and the like of the single teicoplanin components.