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Culture medium for producing teicoplanin by using actinoplanes and culture method

A technology of actinomycetes and teicoplanin, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems affecting the competitiveness of products in the international market and high production costs, and improve the product market. Competitiveness, reduction of production cycle, effect of shortening fermentation cycle

Pending Publication Date: 2019-12-20
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. The production cost of teicoplanin fermentation is relatively high, which affects the competitiveness of the product in the international market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Seed cultivation

[0053] Seed medium composition: mother liquor 47L, cottonseed peptone 12kg, rice protein powder 4kg, three insect powder 2kg, ammonium sulfate 0.3kg, light calcium carbonate 1kg; adding water once, the effective volume of seed liquid is 1m 3 .

[0054] First, sterilize the seed medium and cool it down to 20-25°C, and keep the pressure with sterile air, and then, under the protection of the flame, insert the 10L inoculum of the cultured actinomycetes shake flask fermentation broth into the seed culture Seed culture in the medium. The cell concentration of Actinomycetes mobilis in shake flask fermentation broth was 20%; microscopic examination showed no miscellaneous bacteria.

[0055] Seed culture conditions: tank pressure 0.03 ~ 0.06MPa; tank temperature 27 ~ 28 ℃; air flow: 30m 3 / h; stirring speed 60r / min. After the seed culture is over, the cell concentration is 14%, the pH is 6.9, and the culture time is 42 hours.

[0056] , fermentation c...

Embodiment 2

[0073] 1. Seed cultivation

[0074] Seed medium composition: mother liquor 48L, cottonseed peptone 16kg, rice protein powder 7kg, three insect powder 5kg, ammonium sulfate 0.7kg, light calcium carbonate 2kg; adding water once, the effective volume of seed liquid is 1m 3 .

[0075] First, sterilize the seed medium and cool it down to 20-25°C, and keep the pressure with sterile air, and then, under the protection of the flame, insert the 11L inoculum of the cultured Actinomycetes mobilis shake flask fermentation broth into the seed culture Seed culture in the medium. The concentration of actinomycetes in the shake flask fermentation broth was 27%; there was no miscellaneous bacteria in the microscopic examination.

[0076] Seed culture conditions: tank pressure 0.03 ~ 0.06MPa; tank temperature 27 ~ 28 ℃; air flow: 30m 3 / h; stirring speed 60r / min. After the seed culture is over, the cell concentration is 18%, the pH is 6.8, and the culture time is 43 hours.

[0077] , ferme...

Embodiment 3

[0094] 1. Seed cultivation

[0095] Seed medium composition: mother liquor 50L, cottonseed peptone 12kg, rice protein powder 5kg, three insect powder 2kg, ammonium sulfate 0.5kg, light calcium carbonate 4kg; add water once, the effective volume of seed liquid is 1m 3 .

[0096] First, sterilize the seed medium and cool it down to 20-25°C, keep the pressure with sterile air, and then, under the protection of the flame, insert the 15L inoculum of the cultured actinomycetes shake flask fermentation liquid into the seed culture Seed culture in the medium. The concentration of actinomycetes in the shake flask fermentation broth was 24%; there was no miscellaneous bacteria in the microscopic examination.

[0097] Seed culture conditions: tank pressure 0.03 ~ 0.06MPa; tank temperature 27 ~ 28 ℃; air flow: 40m 3 / h; stirring speed 75r / min. After the seed culture is over, the cell concentration is 16%, the pH is 6.7, and the culture time is 42 hours.

[0098] , fermentation cultur...

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PUM

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Abstract

The invention relates to a culture medium for producing teicoplanin by using actinoplanes and a culture method. The culture medium comprises a seed culture medium and a fermentation culture medium. Byusing a culture medium recipe and the culture method provided by the invention, the teicoplanin fermentation unit can be improved; the fermentation period can be shortened; the fermentation cost canbe reduced; the environment influence on raw and auxiliary material sources is reduced to the maximum degree; the sufficient supply of raw and auxiliary materials is ensured; and the stable and efficient production of the teicoplanin is realized.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a culture method for producing teicoplanin by using actinomycetes. Background technique [0002] Teicoplanin is a bactericidal glycopeptide antibiotic produced by the fermentation of Actinomycetes mobilis. It inhibits the growth of bacteria by inhibiting the synthesis of bacterial cell walls, and is mainly effective against G+ bacteria and anaerobic cocci. Teicoplanin and vancomycin belong to glycopeptide antibiotics, but the half-life of teicoplanin is longer than that of vancomycin (47 hours vs. 11 hours), and the damage to renal function is less than that of vancomycin, while the therapeutic effect is more obvious , and rarely cause allergic reactions caused by glycopeptide antibiotics, the advantages are significant. [0003] At present, the fermentation production of teicoplanin in China adopts the secondary fermentation mode...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02C12R1/045
CPCC12N1/20C12P21/02C12P21/005C07K9/00
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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