The invention discloses a paint for a fruit and vegetable fresh-keeping thermal insulation paper box and a preparation method thereof. The paint is composed of the following raw materials in parts by weight: 10 to 20 parts of oxidized corn starch, 40 to 80 parts of water, 0.5 to 1 part of adsorbent, 0.1 to 0.2 part of antiseptic, and 0.006 to 0.008 part of viscosity regulator, wherein the adsorbent is zeolite powder that carries with potassium permanganate and alumina, and is composed of the following raw materials in parts by weight: 5 to 7 parts of potassium permanganate, 0.4 to 0.6 part of alumina, 80 to 100 parts of water, and 120 to 160 parts of zeolite powder. The paint is prepared by the following steps: mixing potassium permanganate, alumina, and water according to a certain ratio, then adding zeolite, soaking, stirring to obtain the adsorbent, mixing oxidized corn starch and water according to a certain ratio, then adding the adsorbent and antiseptic, heating the system to a temperature of 78 to 83 DEG C until the system is converted into paste, finally adding an viscosity regulator, and evenly stirring. The fresh keeping effect is prominently improved by the paint.