Kit used for detecting beta-lactamase in dairy and detection method thereof

Abstract

The invention discloses a kit used for detecting beta-lactamase in dairy and a detection method thereof. The kit is composed of two parts of a detection main body and a detection reagent, the detection main body is a coated 96 orifice plate containing detection thalline, a soda acid indicator and nutrients required for thalline growth, and the detection reagent is an organic solution containing beta-lactam antibiotics. The detection thalline is a bacillus stearothermophilus suspension; the soda acid indicator is bromcresol purple, and the detection reagent is piperacillin dissolved by acetonitrile. The kit is based on a microbe growth inhibition factor releasing method, color change can reflect the microbe growth state, so that the beta-lactamase content case in milk can be indirectly displayed. The kit has the characteristics of simple operation, rapidity, high efficiency and accuracy, and has characteristics of low equipment requirement, no requirement for professional operation, clear result interpretation.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a test kit for β-lactamase added in dairy products and a detection method thereof. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly, and fresh milk and dairy products have become an important part of people's daily food. However, the widespread use of antibiotics in modern animal husbandry inevitably results in antibiotic residues in milk. β-lactamase antibiotics are a kind of spectrum antibiotics derived from microorganisms, which are widely used in the prevention and treatment of livestock and poultry diseases. In order to increase the production of animal food, some are also used as feed additives, which greatly increases the risk of antibiotic residues in food and the environment, causing subsequent human allergic reactions, microbial resistance, pr...

AI Technical Summary

Problems solved by technology

[0004] For β-lactamase, the principles of existing detection methods are mostly based on ELISA, physical and chemical methods, microbial methods and instrumental detection. The detection sensitivity of ELISA depends on the purity and specificity of receptors and ligands and the cost. High; the results of physical and chemical methods are subject to the operation of personnel to a certain extent; instrumental methods require specific equipment and the detection cost is expensive; microbial methods have better overall results in the detection of β-lactamases, such as the paper recommended by NCCLS in the United States The slice diffusion method, but the interpretation of the results of the microbiological method is based on the observation of the inhibition zone, which is not very clear in the interpretation of the results, and there will be interpretation errors due to human factors