The invention discloses a multifunctional surface
plasma coupled emission
fluorescence and Raman
detector and a detection method thereof, relating to detection of fluorescent spectrum, Raman spectrum,
fluorescent imaging and fluorescent
microscopic imaging. The
detector comprises an angle distinguishing module, an excited light path module, a fluorescent spectrum detection module, a Raman spectrumdetection module, an imaging detection module and a
microscopic imaging detection module, wherein the modules are separately fixed on corresponding rotary tables through optical guide rails, and canrotate simultaneously or independently around a
prism and a membrane sample on the
prism, excitation signals of different angles and emission signals of different angles can be generated by rotating the modules, and a
square pyramid mirror is used as a
coupling element and combines with the detection modules so as to enable the
detector to acquire surface enhanced
fluorescence and Raman, spectrumand imaging information and so on of the same sample simultaneously without using a light splitting
system. The detector has a simple structure, is low-cost, is easy to operate, and is easy to promote.